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In plants, RNA-mediated silencing pathways have diversified in unique ways. This study elucidates the specific functions of some of the key regulators in development, chromatin structure, and pathogen defense.
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Keywords {🔍}
dcl, view, article, rdr, google, scholar, plants, rna, sirnas, methylation, sirna, figure, arabidopsis, mutant, silencing, dna, ago, sequences, small, genes, mutants, plant, endogenous, hen, gene, atsn, loci, atg, wildtype, control, cell, detected, analysis, virus, shown, science, nucleotides, mirna, function, proteins, levels, chromatin, formation, sites, plos, data, zilberman, genome, rnas, protein,
Topics {✒️}
health plos biol 2 cookies plos ethid-ium bromide-stained gels late flowering phenotype small rna-generating systems short integuments1/suspensor1/carpel factory computational methods rna-induced silencing complex sirna-generating systems exist endogenous sirna-generating systems flowering time short interfering rna small rna-directed pathways small rna-generating pathways ethidium bromide-stained gels open-access article distributed rna-dependent rna polymerases rna-dependent rna polymerase rna-directed rna polymerase histone methyltransferase systems end-labeled dna oligonucleotide end-labeled dna oligonucleotides sirna-guided complex virus-induced small rnas sirna-forming capability correlated rna-directed dna methylation exclusive double-stranded rna computational identification computational resources methods plant materials arabidopsis thaliana reveal wild-type la-er plants transgene-induced rna silencing small rna pathways t-dna insertion lines 35s promoter-driven expression dcl3–gfp construct contained arabidopsis inflo-rescence tissue methylation-sensitive restriction endonucleases β-tubulin-specific probe rna silencing-defective mutants class iii hd-zip post-transcriptional rnai serves high-molecular weight rnas untreated wild-type plants t-dna insertion sites k4-methylated histone h3 large ago-related families post-transcriptional gene silencing
Questions {❓}
- If AGO4 and HEN1 function downstream of siRNA formation, why do siRNAs derived from some sites (AtSN1 and 5S rDNA) accumulate to such low levels in ago4 and hen1 mutants?
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