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We began analyzing https://link.springer.com/article/10.1245/ASO.2005.04.010, but it redirected us to https://link.springer.com/article/10.1245/ASO.2005.04.010. The analysis below is for the second page.

Title[redir]:
Expression of the Transcription Factors Snail, Slug, and Twist and Their Clinical Significance in Human Breast Cancer | Annals of Surgical Oncology
Description:
Background Slug, Snail, and Twist are transcription factors that regulate the expression of tumor suppressors such as E-cadherin. We examined the distribution and expression of these three molecules together with the methylation of the Twist gene promoter in human breast cancer to elucidate their clinical significance. Methods Frozen sections from breast cancer primary tumors (tumor, n = 114; background, n = 30) were immunostained with Slug, Snail, and Twist antibodies. RNA was reverse-transcribed, quantified, and analyzed by quantitative polymerase chain reaction (Q-PCR). Results were expressed as copy number of transcript per 50 ng of RNA (standardized against β-actin). Results Immunohistochemistry revealed that all three molecules were stained in mammary tissues, with an increase in Twist within tumor tissues; this was supported by Q-PCR analysis. Q-PCR analysis showed that Slug was elevated with increasing tumor grade and prognostic indices. Twist was elevated with increasing nodal involvement (tumor-node-metastasis status). Conversely, Snail was reduced in expression corresponding with prognostic indices and tumor grade. Increased levels of Slug were associated with tumors from patients with metastatic disease or disease recurrence, and increased expression of Twist was associated with tumors from patients who had died from breast cancer. It is interesting to note that Snail expression was significantly reduced in patients with a poor outcome and those who had node-positive tumors. In addition, tumors exhibited methylation of the Twist promoter. Conclusions These data demonstrate that all three transcription factors have inappropriate expression in breast cancer and that this may play a part in the progression of human breast tumors.

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myogenic basic helix-loop-helix month download article/chapter dc%2bd38xlvvoisrs%3d 12095949 10 cano ma perez-moreno n-cadherin promotes motility transcription factor snail e-cadherin gene expression q-pcr analysis showed gareth watkins bsc & wen full article pdf transcription factors snail cooperative e-box regulation mansel wg jiang zinc-finger protein breast cancer cells human breast cancer cancer research uk epithelial mesenchymal interactions related subjects tumor-node-metastasis status breast cancer progression epithelial-mesenchymal transition epithelial-mesenchymal transitions privacy choices/manage cookies article annals snail/slug family hamamori hy wu human aromatase gene e-cadherin expression human breast tumors human breast tissue q-pcr analysis transcription factors melanoma cells involves article log surgical oncology aims circulating tumor cells article martin dimerization partners determine lymph node status article cite emt markers snail breast cancer snail precedes slug european economic area increasing nodal involvement type ii collagen kass mk baylies walterhouse pm iannaccone ductal lavage fluid

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