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We began analyzing https://veterinaryresearch.biomedcentral.com/articles/10.1186/s13567-025-01495-y, but it redirected us to https://veterinaryresearch.biomedcentral.com/articles/10.1186/s13567-025-01495-y. The analysis below is for the second page.

Title[redir]:
Dynamic interplay between RNA N6-methyladenosine modification and porcine reproductive and respiratory syndrome virus infection | Veterinary Research | Full Text
Description:
N6-methyladenosine (m6A) has attracted significant attention for its role in regulating the complex interaction between viruses and host cells. Porcine reproductive and respiratory syndrome virus (PRRSV) is a significant pathogen affecting swine health worldwide. Here, we first identified seven m6A-enriched peaks in PRRSV genomic RNA by m6A RNA immunoprecipitation sequencing (m6A-seq). Moreover, functional analyses revealed a positive correlation between the m6A modification level and PRRSV replication. Treatment with the universal methylation inhibitor 3-deazaadenosine (3-DAA) effectively suppressed PRRSV replication in a dose-dependent manner. Furthermore, m6A-seq was also used to determine the m6A landscape of the transcriptome in PAMs infected with pandemic or highly pathogenic PRRSV strains. Among the 4677 transcripts exhibiting altered m6A modification levels, the MAPK14 gene and the p38/MAPK signalling pathway emerged as preliminary targets of m6A-mediated epigenetic regulation during PRRSV infection. These findings provide new insights into the epigenetic mechanisms underlying PRRSV infection and may facilitate the development of anti-PRRSV therapeutics.

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Keywords {πŸ”}

prrsv, cells, viral, pubmed, modification, rna, virus, infection, google, scholar, wang, mapk, expression, signalling, replication, mrna, cas, levels, genes, zhang, host, peaks, pathways, gene, modifications, immune, central, role, figure, viruses, antiviral, infected, pathway, regulation, cell, porcine, additional, liu, china, strain, analysis, pathogenic, pmapk, reproductive, respiratory, syndrome, significant, responses, protein, file,

Topics {βœ’οΈ}

crispr/cas-based epitranscriptomic editing tlr4/nf-kappab/mapk pathways p38/mapk undergoes phosphorylation n6-methyladenosine demethylase fto p38/mapk signalling pathway rna n6-methyladenosine modification viral-derived nucleic acids smallest p-adjusted values p38/mapk signalling pathways state privacy rights repeated freezeβ€’thaw cycles m6a-specific merip-seq analysis pi3k-akt signalling pathway m6a-regulated cis-elements m6a-mediated epigenetic regulation m6a-based antiviral strategies mapk-related signalling pathways nonstructural protein-coding regions yu-chuan yang cells overexpressing mettl14 host immune-related transcripts mediates cetuximab resistance privacy choices/manage cookies central metabolic hub nsp2-encoding region contained key immune-related genes p38 mapk pathway p38/mapk pathway full size image bmc mapk signalling pathway prrsv-host cell interactions suggesting host-specific adaptation mystery swine disease evade immune responses signalling pathway plays hen-l1-infected cells hen-l1-infected pams prrsv-infected marc145 cells prrsv-infected pam cells differential m6a levels species-specific m6a readers online tool sramp il-1beta induced highly pathogenic variant yu-fei zhan subsequent deep sequencing regulating immune responses references murakami highly pathogenic strain

Questions {❓}

  • Wang W, Jin Y, Xie Z, He M, Li J, Wang Z, Ma S, Zhang W, Tong J (2024) When animal viruses meet N6-methyladenosine (m6A) modifications: for better or worse?

Schema {πŸ—ΊοΈ}

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      headline:Dynamic interplay between RNA N6-methyladenosine modification and porcine reproductive and respiratory syndrome virus infection
      description:N6-methyladenosine (m6A) has attracted significant attention for its role in regulating the complex interaction between viruses and host cells. Porcine reproductive and respiratory syndrome virus (PRRSV) is a significant pathogen affecting swine health worldwide. Here, we first identified seven m6A-enriched peaks in PRRSV genomic RNA by m6A RNA immunoprecipitation sequencing (m6A-seq). Moreover, functional analyses revealed a positive correlation between the m6A modification level and PRRSV replication. Treatment with the universal methylation inhibitor 3-deazaadenosine (3-DAA) effectively suppressed PRRSV replication in a dose-dependent manner. Furthermore, m6A-seq was also used to determine the m6A landscape of the transcriptome in PAMs infected with pandemic or highly pathogenic PRRSV strains. Among the 4677 transcripts exhibiting altered m6A modification levels, the MAPK14 gene and the p38/MAPK signalling pathway emerged as preliminary targets of m6A-mediated epigenetic regulation during PRRSV infection. These findings provide new insights into the epigenetic mechanisms underlying PRRSV infection and may facilitate the development of anti-PRRSV therapeutics.
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      email:[email protected]
PostalAddress:
      name:College of Life Sciences, School of Life Sciences and Green Development, Hebei University, Baoding, China
      name:College of Life Sciences, School of Life Sciences and Green Development, Hebei University, Baoding, China
      name:College of Life Sciences, School of Life Sciences and Green Development, Hebei University, Baoding, China
      name:College of Life Sciences, School of Life Sciences and Green Development, Hebei University, Baoding, China
      name:College of Life Sciences, School of Life Sciences and Green Development, Hebei University, Baoding, China
      name:College of Life Sciences, School of Life Sciences and Green Development, Hebei University, Baoding, China
      name:College of Life Sciences, School of Life Sciences and Green Development, Hebei University, Baoding, China
      name:College of Life Sciences, School of Life Sciences and Green Development, Hebei University, Baoding, China
      name:College of Life Sciences, School of Life Sciences and Green Development, Hebei University, Baoding, China
      name:College of Veterinary Medicine, Hebei Agriculture University, Baoding, China
      name:State Key Laboratory for Animal Disease Control and Prevention, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin, China
      name:Hebei Provincial Hospital of Traditional Chinese Medicine, Shijiazhuang, China
      name:Neural Academy of Traditional Chinese Medicine, Hebei University of Chinese Medicine, Shijiazhuang, China
      name:College of Veterinary Medicine, Hebei Agriculture University, Baoding, China
      name:College of Life Sciences, School of Life Sciences and Green Development, Hebei University, Baoding, China

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