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DOI . ORG {}

  1. Analyzed Page
  2. Matching Content Categories
  3. CMS
  4. Monthly Traffic Estimate
  5. How Does Doi.org Make Money
  6. Keywords
  7. Topics
  8. Questions
  9. Schema
  10. Social Networks
  11. External Links
  12. Analytics And Tracking
  13. Libraries
  14. Hosting Providers
  15. CDN Services

We began analyzing https://veterinaryresearch.biomedcentral.com/articles/10.1186/s13567-015-0155-6, but it redirected us to https://veterinaryresearch.biomedcentral.com/articles/10.1186/s13567-015-0155-6. The analysis below is for the second page.

Title[redir]:
Eimeria bovis-triggered neutrophil extracellular trap formation is CD11b-, ERK 1/2-, p38 MAP kinase- and SOCE-dependent | Veterinary Research | Full Text
Description:
Eimeria bovis is an important coccidian parasite that causes high economic losses in the cattle industry. We recently showed that polymorphonuclear neutrophils (PMN) react upon E. bovis sporozoite exposure by neutrophil extracellular trap (NET) formation. We focused here on the molecular mechanisms that are involved in this process. The sporozoite encounter led to an enhanced surface expression of neutrophil CD11b suggesting a potential role of this receptor in E. bovis-mediated NETosis. Antibody-mediated blockage of CD11b confirmed this assumption and led to a significantly decreased sporozoite-triggered NET. In addition, E. bovis-induced NETosis was found to be Ca2+-dependent since the inhibition of store-operated calcium entry (SOCE) significantly diminished NET. Furthermore, NADPH oxidase, neutrophil elastase (NE) and myeloperoxidase (MPO) were confirmed as key molecules in sporozoite-triggered NETosis, as inhibition thereof blocked parasite-triggered NET. PMN degranulation analyses revealed a significant release of matrix metalloprotease-9 containing granules upon sporozoite exposure. We further show a significantly enhanced phosphorylation of ERK1/2 and p38 MAPK in sporozoite-exposed PMN indicating a key role of this signaling pathway in E. bovis-mediated NETosis. Accordingly, ERK 1/2 and p38 MAPK inhibition led to a significant decrease in NET formation. Finally, we demonstrate that sporozoite-induced NETosis is neither a stage-, species-, nor host-specific process.

Matching Content Categories {📚}

  • Science
  • Telecommunications
  • Education

Content Management System {📝}

What CMS is doi.org built with?

Custom-built

No common CMS systems were detected on Doi.org, and no known web development framework was identified.

Traffic Estimate {📈}

What is the average monthly size of doi.org audience?

🚄 Respectable Traffic: 10k - 20k visitors per month


Based on our best estimate, this website will receive around 10,019 visitors per month in the current month.
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How Does Doi.org Make Money? {💸}

We find it hard to spot revenue streams.

Not all websites are made for profit; some exist to inform or educate users. Or any other reason why people make websites. And this might be the case. Doi.org could be getting rich in stealth mode, or the way it's monetizing isn't detectable.

Keywords {🔍}

pmn, net, bovis, sporozoites, min, formation, pubmed, netosis, article, bovine, google, scholar, neutrophil, extracellular, cas, cdb, controls, figure, mpo, erk, data, parasite, eimeria, mapk, sigmaaldrich, antibodies, exposure, structures, mmp, nadph, oxidase, significant, cell, positive, analyzed, sporozoite, involved, traps, medium, central, hermosilla, release, mechanism, oocysts, control, inhibition, cells, gondii, arloingi, negative,

Topics {✒️}

contributed reagents/materials/analysis tools springer nature anti-mouse hrp-conjugated antisera poly-l-lysine-treated coverslips ne-chromogenic substrate analysis phase-contrast inverted microscope neutrophil extracellular trap soce-dependent tamara muñoz-caro increases l-selectin shedding store-operated calcium entry varioskan flash® varioskan flash figures 4a quantitative parasite-entrapment-assay strict host-specific parasite pinto-da-silva lh parasite-triggered net formation madin-darby bovine kidney store-operated ca2+ entry bovis-induced net formation bovis sporozoite-induced net bovis sporozoite-induced netosis sporozoite-triggered net formation neutrophil extracellular traps dna-derived fluorescence intensities bovis-triggered netosis highlighting sporozoite-mediated net formation extracellular matrix–based mechanism article muñoz-caro references daugschies pico green®-dna staining p38 mapk-signaling pathways antimicrobial peptide/protease activities full size image anti-cd11b antibodies coupled eimeria bovis-oocyst production bovis-mediated net formation pro-inflammatory cytokines anti-mpo antibodies jointly p38-related signaling pathway extracellular dna-positive fibres pmn-mediated immune reactions author information authors cd11b-dependent-net formation netosis-mediated cell death significant parasite-triggered increase raf-mek-erk pathway rabbit anti-bovine mpo eimeria coccidian species german research foundation

Questions {❓}

  • Brinkmann V, Zychlinsky A (2012) Neutrophil extracellular traps: is immunity the second function of chromatin?

Schema {🗺️}

WebPage:
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         headline:Eimeria bovis-triggered neutrophil extracellular trap formation is CD11b-, ERK 1/2-, p38 MAP kinase- and SOCE-dependent
         description: Eimeria bovis is an important coccidian parasite that causes high economic losses in the cattle industry. We recently showed that polymorphonuclear neutrophils (PMN) react upon E. bovis sporozoite exposure by neutrophil extracellular trap (NET) formation. We focused here on the molecular mechanisms that are involved in this process. The sporozoite encounter led to an enhanced surface expression of neutrophil CD11b suggesting a potential role of this receptor in E. bovis-mediated NETosis. Antibody-mediated blockage of CD11b confirmed this assumption and led to a significantly decreased sporozoite-triggered NET. In addition, E. bovis-induced NETosis was found to be Ca2+-dependent since the inhibition of store-operated calcium entry (SOCE) significantly diminished NET. Furthermore, NADPH oxidase, neutrophil elastase (NE) and myeloperoxidase (MPO) were confirmed as key molecules in sporozoite-triggered NETosis, as inhibition thereof blocked parasite-triggered NET. PMN degranulation analyses revealed a significant release of matrix metalloprotease-9 containing granules upon sporozoite exposure. We further show a significantly enhanced phosphorylation of ERK1/2 and p38 MAPK in sporozoite-exposed PMN indicating a key role of this signaling pathway in E. bovis-mediated NETosis. Accordingly, ERK 1/2 and p38 MAPK inhibition led to a significant decrease in NET formation. Finally, we demonstrate that sporozoite-induced NETosis is neither a stage-, species-, nor host-specific process.
         datePublished:2015-03-05T00:00:00Z
         dateModified:2015-03-05T00:00:00Z
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      headline:Eimeria bovis-triggered neutrophil extracellular trap formation is CD11b-, ERK 1/2-, p38 MAP kinase- and SOCE-dependent
      description: Eimeria bovis is an important coccidian parasite that causes high economic losses in the cattle industry. We recently showed that polymorphonuclear neutrophils (PMN) react upon E. bovis sporozoite exposure by neutrophil extracellular trap (NET) formation. We focused here on the molecular mechanisms that are involved in this process. The sporozoite encounter led to an enhanced surface expression of neutrophil CD11b suggesting a potential role of this receptor in E. bovis-mediated NETosis. Antibody-mediated blockage of CD11b confirmed this assumption and led to a significantly decreased sporozoite-triggered NET. In addition, E. bovis-induced NETosis was found to be Ca2+-dependent since the inhibition of store-operated calcium entry (SOCE) significantly diminished NET. Furthermore, NADPH oxidase, neutrophil elastase (NE) and myeloperoxidase (MPO) were confirmed as key molecules in sporozoite-triggered NETosis, as inhibition thereof blocked parasite-triggered NET. PMN degranulation analyses revealed a significant release of matrix metalloprotease-9 containing granules upon sporozoite exposure. We further show a significantly enhanced phosphorylation of ERK1/2 and p38 MAPK in sporozoite-exposed PMN indicating a key role of this signaling pathway in E. bovis-mediated NETosis. Accordingly, ERK 1/2 and p38 MAPK inhibition led to a significant decrease in NET formation. Finally, we demonstrate that sporozoite-induced NETosis is neither a stage-, species-, nor host-specific process.
      datePublished:2015-03-05T00:00:00Z
      dateModified:2015-03-05T00:00:00Z
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         NADPH Oxidase
         Neutrophil Elastase
         Neutrophil Extracellular Trap
         Varioskan Flash
         Coccidian Parasite
         Veterinary Medicine/Veterinary Science
         Virology
         Microbiology
         Immunology
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            address:
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               type:PostalAddress
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      name:Anja Taubert
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            name:Justus Liebig University Giessen
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      name:Institute of Parasitology, Justus Liebig University Giessen, Giessen, Germany
      name:Laboratory of Inflammation Pharmacology, Institute of Pharmacology and Morphophysiology, Faculty of Veterinary Science, Austral University of Chile, Valdivia, Chile
      name:Department of Biology, University of Nariño, Pasto, Colombia
      name:Laboratory of Inflammation Pharmacology, Institute of Pharmacology and Morphophysiology, Faculty of Veterinary Science, Austral University of Chile, Valdivia, Chile
      name:Laboratory of Inflammation Pharmacology, Institute of Pharmacology and Morphophysiology, Faculty of Veterinary Science, Austral University of Chile, Valdivia, Chile
      name:Laboratory of Inflammation Pharmacology, Institute of Pharmacology and Morphophysiology, Faculty of Veterinary Science, Austral University of Chile, Valdivia, Chile
      name:Laboratory of Inflammation Pharmacology, Institute of Pharmacology and Morphophysiology, Faculty of Veterinary Science, Austral University of Chile, Valdivia, Chile
      name:Institute of Parasitology, Justus Liebig University Giessen, Giessen, Germany
      name:Institute of Parasitology, Justus Liebig University Giessen, Giessen, Germany

Social Networks {👍}(2)

External Links {🔗}(306)

Analytics and Tracking {📊}

  • Google Tag Manager

Libraries {📚}

  • Prism.js

Emails and Hosting {✉️}

Mail Servers:

  • mx.zoho.eu
  • mx2.zoho.eu
  • mx3.zoho.eu

Name Servers:

  • josh.ns.cloudflare.com
  • zita.ns.cloudflare.com

CDN Services {📦}

  • Crossref

4.35s.