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DOI . ORG {}

  1. Analyzed Page
  2. Matching Content Categories
  3. CMS
  4. Monthly Traffic Estimate
  5. How Does Doi.org Make Money
  6. Keywords
  7. Topics
  8. Social Networks
  9. External Links
  10. Analytics And Tracking
  11. Libraries
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  13. CDN Services

We began analyzing https://journals.physiology.org/doi/10.1152/ajpregu.2000.279.4.R1165, but it redirected us to https://journals.physiology.org/doi/10.1152/ajpregu.2000.279.4.R1165. The analysis below is for the second page.

Title[redir]:
NF-κB mediates the protein loss induced by TNF-α in differentiated skeletal muscle myotubes | American Journal of Physiology-Regulatory, Integrative and Comparative Physiology | American Physiological Society
Description:
Nuclear factor-κB (NF-κB) regulates the transcription of a variety of genes involved in immune responses, cell growth, and cell death. However, the role of NF-κB in muscle biology is poorly understood. We recently reported that tumor necrosis factor-α (TNF-α) rapidly activates NF-κB in differentiated skeletal muscle myotubes and that TNF-α acts directly on the muscle cell to induce protein degradation. In the present study, we ask whether NF-κB mediates the protein loss induced by TNF-α. We addressed this problem by creating stable, transdominant negative muscle cell lines. C2C12 myoblasts were transfected with viral plasmid constructs that induce overexpression of mutant I-κBα proteins that are insensitive to degradation via the ubiquitin-proteasome pathway. These mutant proteins selectively inhibit NF-κB activation. We found that differentiated myotubes transfected with the empty viral vector (controls) underwent a drop in total protein content and in fast-type myosin heavy-chain content during 72 h of exposure to TNF-α. In contrast, total protein and fast-type myosin heavy-chain levels were unaltered by TNF-α in the transdominant negative cell lines. TNF-α did not induce apoptosis in any cell line, as assessed by DNA ladder and annexin V assays. These data indicate that NF-κB is an essential mediator of TNF-α-induced catabolism in differentiated muscle cells.

Matching Content Categories {📚}

  • Health & Fitness
  • Science
  • Fitness & Wellness

Content Management System {📝}

What CMS is doi.org built with?

Custom-built

No common CMS systems were detected on Doi.org, and no known web development framework was identified.

Traffic Estimate {📈}

What is the average monthly size of doi.org audience?

🌟 Strong Traffic: 100k - 200k visitors per month


Based on our best estimate, this website will receive around 100,420 visitors per month in the current month.

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How Does Doi.org Make Money? {💸}

We're unsure if the website is profiting.

Some websites aren't about earning revenue; they're built to connect communities or raise awareness. There are numerous motivations behind creating websites. This might be one of them. Doi.org might have a hidden revenue stream, but it's not something we can detect.

Keywords {🔍}

tnfα, nfκb, myotubes, muscle, protein, cell, differentiated, cells, iκbα, mhcf, loss, activation, iκbαδn, negative, control, american, transfected, levels, apoptosis, data, journal, physiology, skeletal, transdominant, dna, ngml, vector, overexpressed, degradation, mutant, pcmv, fig, content, present, tnfαinduced, wasting, studies, effects, ser, induce, empty, total, inflammatory, nuclear, death, lines, myoblasts, proteins, pathway, cytokine,

Topics {✒️}

r1165 sections pdf nf-κb-binding dna probe overexpressed i-κbαs32/36a exhibited tnf-α-induced muscle wasting tnf-α-induced protein loss i-κbαs32/36a inhibited activation american physiological society tnf-α/nf-κb signaling tnf-α activated nf-κb inhibit nf-κb activation rapidly activates nf-κb mouse recombinant tnf-α jun-nh2-terminal kinases tumor necrosis factor-α tnf-α-induced catabolism dna-binding proteins p50 tnf-α stimulation increases higher tnf-α concentrations inhibits nuclear factor-κb tnf-α-receptor complex aps website aps member activated nf-κb dimer physiological genomics journal western blot analysis fibroblast-derived l929 cells protein assay kit mutant i-κbα proteins test nf-κb mediation nf-κb requires phosphorylation activate nf-κb directly determining nf-κb effects download figuredownload powerpoint tnf-α acts directly tnf-α stimulates apoptosis tnf-α-induced apoptosis tnf-α induced apoptosis mutant i-κbα protein inhibitory protein i-κbα journals quick search circulating tnf-α levels time-matched control myotubes tnf-α signal dna ladder detection evaluate tnf-α effects transdominant negative mutant applied physiology journal nf-κb mediates metabolism american journal annexin v-fitc

Social Networks {👍}(4)

External Links {🔗}(524)

Analytics and Tracking {📊}

  • Crazy Egg
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Libraries {📚}

  • Dropzone.js
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Emails and Hosting {✉️}

Mail Servers:

  • mx.zoho.eu
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Name Servers:

  • josh.ns.cloudflare.com
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CDN Services {📦}

  • Feathr
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