
DOI . ORG {
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Title[redir]:
Mechanisms, Applications, and Challenges of Insect RNA Interference | Annual Reviews
Description:
The RNA interference (RNAi) triggered by short/small interfering RNA (siRNA) was discovered in nematodes and found to function in most living organisms. RNAi has been widely used as a research tool to study gene functions and has shown great potential for the development of novel pest management strategies. RNAi is highly efficient and systemic in coleopterans but highly variable or inefficient in many other insects. Differences in double-stranded RNA (dsRNA) degradation, cellular uptake, inter- and intracellular transports, processing of dsRNA to siRNA, and RNA-induced silencing complex formation influence RNAi efficiency. The basic dsRNA delivery methods include microinjection, feeding, and soaking. To improve dsRNA delivery, various new technologies, including cationic liposome–assisted, nanoparticle-enabled, symbiont-mediated, and plant-mediated deliveries, have been developed. Major challenges to widespread use of RNAi in insect pest management include variable RNAi efficiency among insects, lack of reliable dsRNA delivery methods, off-target and nontarget effects, and potential development of resistance in insect populations.
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Keywords {🔍}
google, scholar, rna, insect, rnai, biol, interference, mol, sci, genes, dsrna, drosophila, biochem, insects, gene, pest, silencing, zhang, wang, cell, palli, doublestranded, systemic, zhu, rep, plos, beetle, virgifera, delivery, physiol, pathway, manag, locust, mechanisms, sid, christiaens, rev, expression, biotechnol, control, nat, smagghe, response, cells, lee, development, annual, vol, uptake, tribolium,
Topics {✒️}
dsrna-selective dsrna-gated channel alien double-stranded rnas long double-stranded rnas ingested double-stranded rnas extracellular double-stranded rna clathrin-dependent endocytosis plays full crop protection dual-layer transposon repression rnai-mediated antiviral immunity soaking rnai-mediated modification piwi-interacting small rnas double-stranded rna activity double-stranded rna degradation double-stranded rna uptake double-stranded rna injection jean-marie delpuech vol trauma-free sustainable delivery precise rnai-mediated silencing mosquito-specific microrna-1890 targets pea aphid–buchnera symbiosis large-scale field application dsrna-mediated genetic interference rnai-mediated crop protection rnai-mediated gene silencing rnai-based gene silencing sirna-mediated gene silencing protein-coupled receptors required grossi-de-sa mf 2017 rnai-based reverse genetics dsrna-resistant insect population double-stranded rna chitosan/dsrna nanoparticles clathrin-mediated endocytosis clathrin-dependent endocytosis somatic transposon-derived pirnas african malaria mosquito rna interference technology phased pirna production western corn rootworm sirna/mirna silencing pathways small rna pathways cotton boll weevil targeted gene suppression generation transgenic cotton red flour beetle juvenile leaf beetles cockroach blattella germanica tissue-dependent susceptibility small hive beetle persistent virus infection
Questions {❓}
- Larval RNAi in Drosophila?
- Super-induction of Dicer-2 expression by alien double-stranded RNAs: an evolutionary ancient response to viral infection?
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- How much does http://scholar.google.com/scholar_lookup?title=Precise+RNAi-mediated+silencing+of+metabolically+active+proteins+in+the+defense+secretions+of+juvenile+leaf+beetles&author=RR+Bodemann&author=P+Rahfeld&author=M+Stock&author=M+Kunert&author=N+Wielsch&journal=Proc.+Biol.+Sci.&volume=279&pages=4126-34&publication_year=2012& pull in?
- What's the monthly income of http://scholar.google.com/scholar_lookup?title=Characterizing+the+mechanism+of+action+of+double-stranded+RNA+activity+against+western+corn+rootworm+%28Diabrotica+virgifera+virgifera+LeConte%29&author=R+Bolognesi&author=P+Ramaseshadri&author=J+Anderson&author=P+Bachman&author=W+Clinton&journal=PLOS+ONE&volume=7&pages=e47534&publication_year=2012&?
- Revenue of http://scholar.google.com/scholar_lookup?title=Comparative+genomics+of+small+RNA+regulatory+pathway+components+in+vector+mosquitoes&author=CL+Campbell&author=WC+Black&author=AM+Hess&author=BD+Foy&journal=BMC+Genom&volume=9&pages=425&publication_year=2008&
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- How much does http://scholar.google.com/scholar_lookup?title=Origins+and+mechanisms+of+miRNAs+and+siRNAs&author=RW+Carthew&author=EJ.+Sontheimer&journal=Cell&volume=136&pages=642-55&publication_year=2009& generate monthly?
- What are the earnings of http://scholar.google.com/scholar_lookup?title=dsRNA+degradation+in+the+pea+aphid+%28Acyrthosiphon+pisum%29+associated+with+lack+of+response+in+RNAi+feeding+and+injection+assay&author=O+Christiaens&author=L+Swevers&author=G+Smagghe&journal=Peptides&volume=53&pages=307-14&publication_year=2014&?
- What's the monthly money flow for http://scholar.google.com/scholar_lookup?title=Targeting+symbiosis-related+insect+genes+by+RNAi+in+the+pea+aphid%E2%80%93Buchnera+symbiosis&author=SH+Chung&author=X+Jing&author=Y+Luo&author=AE+Douglas&journal=Insect+Biochem.+Mol.+Biol.&volume=95&pages=55-63&publication_year=2018&?
- Financial intake of http://scholar.google.com/scholar_lookup?title=Systemic+RNAi+of+the+cockroach+vitellogenin+receptor+results+in+a+phenotype+similar+to+that+of+the+Drosophila+yolkless+mutant&author=L+Ciudad&author=MD+Piulachs&author=X+Bell%C3%A9s&journal=FEBS+J&volume=273&pages=325-35&publication_year=2006&
- How much does http://scholar.google.com/scholar_lookup?title=Molecular+mechanisms+influencing+efficiency+of+RNA+interference+in+insects&author=AM+Cooper&author=K+Silver&author=JZ+Zhang&author=Y+Park&author=KY+Zhu&journal=Pest+Manag.+Sci.&volume=75&pages=18-28&publication_year=2019& pull in?
- How much revenue does http://scholar.google.com/scholar_lookup?title=Viruses+and+RNA+interference%3A+issues+and+controversies&author=BR.+Cullen&journal=J.+Virol.&volume=88&pages=12934-36&publication_year=2014& generate?
- Monthly income for http://scholar.google.com/scholar_lookup?title=An+endogenous+small+interfering+RNA+pathway+in+Drosophila&author=B+Czech&author=CD+Malone&author=R+Zhou&author=A+Stark&author=C+Schlingeheyde&journal=Nature&volume=453&pages=798-802&publication_year=2008&
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- How much does http://scholar.google.com/scholar_lookup?title=pH+gradients+in+lepidopteran+midgut&author=JA.+Dow&journal=J.+Exp.+Biol.&volume=172&pages=355-75&publication_year=1992& make?
- What's http://scholar.google.com/scholar_lookup?title=Phylogenetic+origin+and+diversification+of+RNAi+pathway+genes+in+insects&author=D+Dowling&author=T+Pauli&author=A+Donath&author=K+Meusemann&author=L+Podsiadlowski&journal=Genome+Biol.+Evol.&volume=8&pages=3784-93&publication_year=2016&'s gross income?
- What's the financial outcome of http://scholar.google.com/scholar_lookup?title=Potent+and+specific+genetic+interference+by+double-stranded+RNA+in+Caenorhabditis+elegans&author=A+Fire&author=S+Xu&author=MK+Montgomery&author=SA+Kostas&author=SE+Driver&author=CC+Mello&journal=Nature&volume=391&pages=806-11&publication_year=1998&?
- How much does http://scholar.google.com/scholar_lookup?title=Endogenous+siRNAs+derived+from+transposons+and+mRNAs+in+Drosophila+somatic+cells&author=M+Ghildiyal&author=H+Seitz&author=MD+Horwich&author=CJ+Li&author=TT+Du&journal=Science&volume=320&pages=1077-81&publication_year=2008& pull in monthly?
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- See how much http://scholar.google.com/scholar_lookup?title=Instar-dependent+systemic+RNA+interference+response+in+Leptinotarsa+decemlineata+larvae&author=WC+Guo&author=KY+Fu&author=S+Yang&author=XX+Li&author=GQ+Li&journal=Pestic.+Biochem.+Physiol.&volume=123&pages=64-73&publication_year=2015& makes per month
- Explore the financials of http://scholar.google.com/scholar_lookup?title=Comparison+of+RNAi+knockdown+effect+of+tyramine+receptor+1+induced+by+dsRNA+and+siRNA+in+brains+of+the+honey+bee%2C+Apis+mellifera&author=X+Guo&author=Y+Wang&author=I+Sinakevitch&author=H+Lei&author=BH+Smith&journal=J.+Insect+Physiol.&volume=111&pages=47-52&publication_year=2018&
- How much does http://scholar.google.com/scholar_lookup?title=piRNA-guided+transposon+cleavage+initiates+Zucchini-dependent%2C+phased+piRNA+production&author=BW+Han&author=W+Wang&author=C+Li&author=Z+Weng&author=PD+Zamore&journal=Science&volume=348&pages=817-21&publication_year=2015& net monthly?
- Discover the revenue of http://scholar.google.com/scholar_lookup?title=piRNAs%E2%80%94the+ancient+hunters+of+genome+invaders&author=JV+Hartig&author=Y+Tomari&author=K+F%C3%B6rstemann&journal=Genes+Dev&volume=21&pages=1707-13&publication_year=2007&
- http://scholar.google.com/scholar_lookup?title=Fluorescent+nanoparticle+delivered+dsRNA+toward+genetic+control+of+insect+pests&author=B+He&author=Y+Chu&author=M+Yin&author=K+Mullen&author=C+An&author=J+Shen&journal=Adv.+Mater.&volume=25&pages=4580-84&publication_year=2013&'s revenue stream
- How much revenue does http://scholar.google.com/scholar_lookup?title=RNA+interference+%28RNAi%29+screening+in+Drosophila&author=F+Heigwer&author=F+Port&author=M+Boutros&journal=Genetics&volume=208&pages=853-74&publication_year=2018& bring in?
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- What's the income of http://scholar.google.com/scholar_lookup?title=RNAi+analysis+of+Deformed%2C+proboscipedia+and+Sex+combs+reduced+in+the+milkweed+bug+Oncopeltus+fasciatus%3A+novel+roles+for+Hox+genes+in+the+hemipteran+head&author=CL+Hughes&author=TC.+Kaufman&journal=Development&volume=127&pages=3683-94&publication_year=2000&?
- What is the earnings of http://scholar.google.com/scholar_lookup?title=Large-scale+field+application+of+RNAi+technology+reducing+Israeli+acute+paralysis+virus+disease+in+honey+bees+%28Apis+mellifera%2C+Hymenoptera%3A+Apidae%29&author=W+Hunter&author=J+Ellis&author=D+Vanengelsdorp&author=J+Hayes&author=D+Westervelt&journal=PLOS+Pathog&volume=6&pages=e1001160&publication_year=2010&?
- How much does http://scholar.google.com/scholar_lookup?title=Environmental+RNAi+in+herbivorous+insects&author=S+Ivashuta&author=YJ+Zhang&author=BE+Wiggins&author=P+Ramaseshadri&author=GC+Segers&journal=RNA&volume=21&pages=840-50&publication_year=2015& pull in?
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- How much does http://scholar.google.com/scholar_lookup?title=RNAi+efficiency%2C+systemic+properties%2C+and+novel+delivery+methods+for+pest+insect+control%3A+what+we+know+so+far&author=MR+Joga&author=MJ+Zotti&author=G+Smagghe&author=O+Christiaens&journal=Front.+Physiol.&volume=7&pages=553&publication_year=2016& rake in every month?
- What are the earnings of http://scholar.google.com/scholar_lookup?title=Drosophila+cells+use+nanotube-like+structures+to+transfer+dsRNA+and+RNAi+machinery+between+cells&author=M+Karlikow&author=B+Goic&author=V+Mongelli&author=A+Salles&author=C+Schmitt&journal=Sci.+Rep.&volume=6&pages=27085&publication_year=2016&?
- How profitable is http://scholar.google.com/scholar_lookup?title=Use+of+dsRNA-mediated+genetic+interference+to+demonstrate+that+frizzled+and+frizzled+2+act+in+the+wingless+pathway&author=JR+Kennerdell&author=RW.+Carthew&journal=Cell&volume=95&pages=1017-26&publication_year=1998&?
- How much does http://scholar.google.com/scholar_lookup?title=Development+and+characterization+of+the+first+dsRNA-resistant+insect+population+from+western+corn+rootworm%2C+Diabrotica+virgifera+virgifera+LeConte&author=C+Khajuria&author=S+Ivashuta&author=E+Wiggins&author=L+Flagel&author=W+Moar&journal=PLOS+ONE&volume=13&pages=e0197059&publication_year=2018& make?
- How much profit is http://scholar.google.com/scholar_lookup?title=Parental+RNA+interference+of+genes+involved+in+embryonic+development+of+the+western+corn+rootworm%2C+Diabrotica+virgifera+virgifera+LeConte&author=C+Khajuria&author=AM+Velez&author=M+Rangasamy&author=HC+Wang&author=E+Fishilevich&journal=Insect+Biochem.+Mol.+Biol.&volume=63&pages=54-62&publication_year=2015& making per month?
- What is the monthly revenue of http://scholar.google.com/scholar_lookup?title=The+RNAi+pathway+initiated+by+Dicer-2+in+Drosophila.+Cold+Spring+Harb.+Symp.+Quant&author=K+Kim&author=YS+Lee&author=D+Harris&author=K+Nakahara&author=RW+Carthew&journal=Biol&volume=71&pages=39-44&publication_year=2006&?
- http://scholar.google.com/scholar_lookup?title=RNA+interference%3A+applications+and+advances+in+insect+toxicology+and+insect+pest+management&author=YH+Kim&author=M+Soumaila+Issa&author=AM+Cooper&author=KY+Zhu&journal=Pestic.+Biochem.+Physiol.&volume=120&pages=109-17&publication_year=2015&'s revenue stream
- Explore the financials of http://scholar.google.com/scholar_lookup?title=SID-1+protein+of+Caenorhabditis+elegans+mediates+uptake+of+dsRNA+into+Bombyx+cells&author=I+Kobayashi&author=H+Tsukioka&author=N+Komoto&author=K+Uchino&author=H+Sezutsu&journal=Insect+Biochem.+Mol.+Biol.&volume=42&pages=148-54&publication_year=2012&
- How much money does http://scholar.google.com/scholar_lookup?title=Natural+history-driven%2C+plant-mediated+RNAi-based+study+reveals+CYP6B46%E2%80%99s+role+in+a+nicotine-mediated+antipredator+herbivore+defense&author=P+Kumar&author=SS+Pandit&author=A+Steppuhn&author=IT+Baldwin&journal=PNAS&volume=111&pages=1245-52&publication_year=2014& generate?
- Revenue of http://scholar.google.com/scholar_lookup?title=RNAi-mediated+gene+silencing+in+Rhynchophorus+ferrugineus+%28Oliver%29+%28Coleoptera%3A+Curculionidae%29&author=F+Laudani&author=CP+Strano&author=MG+Edwards&author=A+Malacrino&author=O+Campolo&journal=Open+Life+Sci&volume=12&pages=214-22&publication_year=2017&
- How much does http://scholar.google.com/scholar_lookup?title=Cationic+liposomes+carrying+siRNA%3A+impact+of+lipid+composition+on+physicochemical+properties%2C+cytotoxicity+and+endosomal+escape&author=A+Lechanteur&author=V+Sanna&author=A+Duchemin&author=B+Evrard&author=D+Mottet&author=G+Piel&journal=Nanomaterials&volume=8&pages=E270&publication_year=2018& net monthly?
- How much does http://scholar.google.com/scholar_lookup?title=MicroRNA+genes+are+transcribed+by+RNA+polymerase+II&author=Y+Lee&author=M+Kim&author=JJ+Han&author=KH+Yeom&author=S+Lee&journal=EMBO+J&volume=23&pages=4051-60&publication_year=2004& pull in monthly?
- How much profit does http://scholar.google.com/scholar_lookup?title=Distinct+roles+for+Drosophila+Dicer-1+and+Dicer-2+in+the+siRNA%2FmiRNA+silencing+pathways&author=YS+Lee&author=K+Nakahara&author=JW+Pham&author=K+Kim&author=Z+He&journal=Cell&volume=117&pages=69-81&publication_year=2004& make?
- What's the financial gain of http://scholar.google.com/scholar_lookup?title=RNAi-mediated+antiviral+immunity+in+insects+and+their+possible+application&author=M+Leggewie&author=E.+Schnettler&journal=Curr.+Opin.+Virol.&volume=32&pages=108-14&publication_year=2018&?
- http://scholar.google.com/scholar_lookup?title=Duplication+and+diversification+of+dipteran+Argonaute+genes%2C+and+the+evolutionary+divergence+of+Piwi+and+Aubergine&author=SH+Lewis&author=H+Salmela&author=DJ+Obbard&journal=Genome+Biol.+Evol.&volume=8&pages=507-18&publication_year=2016&'s revenue stream
- http://scholar.google.com/scholar_lookup?title=Two+chitinase+5+genes+from+Locusta+migratoria%3A+molecular+characteristics+and+functional+differentiation&author=D+Li&author=JQ+Zhang&author=Y+Wang&author=XJ+Liu&author=EB+Ma&journal=Insect+Biochem.+Mol.+Biol.&volume=58&pages=46-54&publication_year=2015&'s revenue stream
- What's the profit of http://scholar.google.com/scholar_lookup?title=Endocytic+pathway+mediates+refractoriness+of+insect+Bactrocera+dorsalis+to+RNA+interference&author=XX+Li&author=XL+Dong&author=C+Zou&author=HY+Zhang&journal=Sci.+Rep.&volume=5&pages=8700&publication_year=2015&?
- Monthly income for http://scholar.google.com/scholar_lookup?title=Enhancement+of+larval+RNAi+efficiency+by+over-expressing+Argonaute2+in+Bombyx+mori.+Int.+J.+Biol&author=Z+Li&author=B+Zeng&author=L+Ling&author=J+Xu&author=L+You&journal=Sci&volume=11&pages=176-85&publication_year=2015&
- How much income is http://scholar.google.com/scholar_lookup?title=RNA+interference+knockdown+of+DNA+methyl-transferase+3+affects+gene+alternative+splicing+in+the+honeybee&author=H+Li-Byarlay&author=Y+Li&author=H+Stroud&author=S+Feng&author=TC+Newman&journal=PNAS&volume=110&pages=12750-55&publication_year=2013& earning monthly?
- Get to know http://scholar.google.com/scholar_lookup?title=Super-induction+of+Dicer-2+expression+by+alien+double-stranded+RNAs%3A+an+evolutionary+ancient+response+to+viral+infection%3F&author=J+Lozano&author=E+Gomez-Orte&author=HJ+Lee&author=X+Bell%C3%A9s&journal=Dev.+Genes+Evol.&volume=222&pages=229-35&publication_year=2012&'s earnings
- How much does http://scholar.google.com/scholar_lookup?title=Cholinergic+and+non-cholinergic+functions+of+two+acetylcholinesterase+genes+revealed+by+gene-silencing+in+Tribolium+castaneum.+Sci&author=YH+Lu&author=Y+Park&author=XW+Gao&author=X+Zhang&author=JX+Yao&journal=Rep&volume=2&pages=288&publication_year=2012& gross monthly?
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- What's the revenue for http://scholar.google.com/scholar_lookup?title=Towards+an+understanding+of+the+molecular+basis+of+effective+RNAi+against+a+global+insect+pest%2C+the+whitefly+Bemisia+tabaci&author=Y+Luo&author=Q+Chen&author=J+Luan&author=SH+Chung&author=J+Van+Eck&journal=Insect+Biochem.+Mol.+Biol&volume=88&pages=21-29&publication_year=2017&?
- How much profit is http://scholar.google.com/scholar_lookup?title=Differential+responses+of+migratory+locusts+to+systemic+RNA+interference+via+double-stranded+RNA+injection+and+feeding&author=Y+Luo&author=X+Wang&author=X+Wang&author=D+Yu&author=B+Chen&author=L+Kang&journal=Insect+Mol.+Biol.&volume=22&pages=574-83&publication_year=2013& making per month?
- What's the revenue for http://scholar.google.com/scholar_lookup?title=The+SID-1+double-stranded+RNA+transporter+is+not+required+for+systemic+RNAi+in+the+migratory+locust&author=Y+Luo&author=X+Wang&author=D+Yu&author=L+Kang&journal=RNA+Biol&volume=9&pages=663-71&publication_year=2012&?
- How much money does http://scholar.google.com/scholar_lookup?title=Knocking+down+chitin+synthase+2+by+RNAi+is+lethal+to+the+cotton+boll+weevil&author=LLP+Macedo&author=JD+Antonino+de+Souza&author=RR+Coelho&author=FCA+Fonseca&author=AAP+Firmino&journal=Biotechnol.+Res.+Innov.&volume=1&pages=72-86&publication_year=2017& generate?
- What is the earnings of http://scholar.google.com/scholar_lookup?title=Structure+of+Dicer+and+mechanistic+implications+for+RNAi&author=IJ+Macrae&author=F+Li&author=K+Zhou&author=WZ+Cande&author=JA+Doudna&journal=Cold+Spring+Harb.+Symp.+Quant.+Biol.&volume=71&pages=73-80&publication_year=2006&?
- How much does http://scholar.google.com/scholar_lookup?title=Specialized+piRNA+pathways+act+in+germline+and+somatic+tissues+of+the+Drosophila+ovary&author=CD+Malone&author=J+Brennecke&author=M+Dus&author=A+Stark&author=WR+McCombie&journal=Cell&volume=137&pages=522-35&publication_year=2009& rake in every month?
- How much does http://scholar.google.com/scholar_lookup?title=Silencing+a+cotton+bollworm+P450+monooxygenase+gene+by+plant-mediated+RNAi+impairs+larval+tolerance+of+gossypol&author=YB+Mao&author=WJ+Cai&author=JW+Wang&author=GJ+Hong&author=XY+Tao&journal=Nat.+Biotechnol.&volume=25&pages=1307-13&publication_year=2007& earn?
- How much profit is http://scholar.google.com/scholar_lookup?title=Loqs+and+R2D2+act+sequentially+in+the+siRNA+pathway+in+Drosophila&author=JT+Marques&author=K+Kim&author=PH+Wu&author=TM+Alleyne&author=N+Jafari&author=RW+Carthew&journal=Nat.+Struct.+Mol.+Biol&volume=17&pages=24-30&publication_year=2010& making per month?
- Find out how much http://scholar.google.com/scholar_lookup?title=RNAi+studies+reveal+a+conserved+role+for+RXR+in+molting+in+the+cockroach+Blattella+germanica&author=D+Martin&author=O+Maestro&author=J+Cruz&author=D+Mane-Padros&author=X+Bell%C3%A9s&journal=J.+Insect+Physiol&volume=52&pages=410-16&publication_year=2006& earns monthly
- Find out how much http://scholar.google.com/scholar_lookup?title=Passenger-strand+cleavage+facilitates+assembly+of+siRNA+into+Ago2-containing+RNAi+enzyme+complexes&author=C+Matranga&author=Y+Tomari&author=C+Shin&author=DP+Bartel&author=PD+Zamore&journal=Cell&volume=123&pages=607-20&publication_year=2005& earns monthly
- How much profit does http://scholar.google.com/scholar_lookup?title=Uptake+of+extracellular+double-stranded+RNA+by+SID-2&author=DL+McEwan&author=AS+Weisman&author=CP+Huntert&journal=Mol.+Cell.&volume=47&pages=746-54&publication_year=2012& generate?
- http://scholar.google.com/scholar_lookup?title=Argonaute+proteins%3A+functional+insights+and+emerging+roles&author=G.+Meister&journal=Nat.+Rev.+Genet.&volume=14&pages=447-59&publication_year=2013&'s total income per month
- How much money does http://scholar.google.com/scholar_lookup?title=Larval+RNAi+in+Drosophila%3F&author=SC+Miller&author=SJ+Brown&author=Y+Tomoyasu&journal=Dev.+Genes+Evol.&volume=218&pages=505-10&publication_year=2008& make?
- How much money does http://scholar.google.com/scholar_lookup?title=Dissecting+systemic+RNA+interference+in+the+red+flour+beetle+Tribolium+castaneum%3A+parameters+affecting+the+efficiency+of+RNAi&author=SC+Miller&author=K+Miyata&author=SJ+Brown&author=Y+Tomoyasu&journal=PLOS+ONE&volume=7&pages=e47431&publication_year=2012& generate?
- Financial intake of http://scholar.google.com/scholar_lookup?title=Improving+RNAi+in+the+brown+marmorated+stink+bug%3A+identification+of+target+genes+and+reference+genes+for+RT-qPCR&author=K+Mogilicherla&author=JL+Howell&author=SR+Palli&journal=Sci.+Rep.&volume=8&pages=3720&publication_year=2018&
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- Profit of http://scholar.google.com/scholar_lookup?title=RNAi-mediated+crop+protection+against+insects&author=DR+Price&author=JA.+Gatehouse&journal=Trends+Biotechnol&volume=26&pages=393-400&publication_year=2008&
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- What's the revenue for http://scholar.google.com/scholar_lookup?title=Delivery+of+chitosan%2FdsRNA+nanoparticles+for+silencing+of+wing+development+vestigial+%28vg%29+gene+in+Aedes+aegypti+mosquitoes&author=D+Ramesh+Kumar&author=P+Saravana+Kumar&author=MR+Gandhi&author=NA+Al-Dhabi&author=MG+Paulraj&author=S+Ignacimuthu&journal=Int.+J.+Biol.+Macromol.&volume=86&pages=89-95&publication_year=2016&?
- How much does http://scholar.google.com/scholar_lookup?title=Validation+of+RNA+interference+in+western+corn+rootworm+Diabrotica+virgifera+virgifera+LeConte+%28Coleoptera%3A+Chrysomelidae%29+adults&author=M+Rangasamy&author=BD.+Siegfried&journal=Pest+Manag.+Sci.&volume=68&pages=587-91&publication_year=2012& net monthly?
- Check the income stats for http://scholar.google.com/scholar_lookup?title=dsRNA+uptake+and+persistence+account+for+tissue-dependent+susceptibility+to+RNA+interference+in+the+migratory+locust%2C+Locusta+migratoria&author=D+Ren&author=Z+Cai&author=J+Song&author=Z+Wu&author=S+Zhou&journal=Insect+Mol.+Biol.&volume=23&pages=175-84&publication_year=2014&
- Revenue of http://scholar.google.com/scholar_lookup?title=RNA+interference+in+the+Asian+longhorned+beetle%3A+identification+of+key+RNAi+genes+and+reference+genes+for+RT-qPCR&author=TB+Rodrigues&author=RK+Dhandapani&author=JJ+Duan&author=SR+Palli&journal=Sci.+Rep.&volume=7&pages=8913&publication_year=2017&
- http://scholar.google.com/scholar_lookup?title=Development+of+RNAi+method+for+screening+candidate+genes+to+control+emerald+ash+borer%2C+Agrilus+planipennis&author=TB+Rodrigues&author=LK+Rieske&author=JJ+Duan&author=K+Mogilicherla&author=SR+Palli&journal=Sci.+Rep.&volume=7&pages=7379&publication_year=2017&'s financial summary
- How much does http://scholar.google.com/scholar_lookup?title=RNA+interference+mechanisms+and+applications+in+plant+pathology&author=C+Rosa&author=YW+Kuo&author=H+Wuriyanghan&author=BW+Falk&journal=Annu.+Rev.+Phytopathol.&volume=56&pages=581-610&publication_year=2018& bring in each month?
- How much does http://scholar.google.com/scholar_lookup?title=Conserved+association+of+Argonaute+1+and+2+proteins+with+miRNA+and+siRNA+pathways+throughout+insect+evolution%2C+from+cockroaches+to+flies&author=M+Rubio&author=JL+Maestro&author=M-D+Piulachs&author=X+Bell%C3%A9s&journal=Biochim.+Biophys.+Acta+Gene+Regul.+Mech.&volume=1861&pages=554-60&publication_year=2018& pull in?
- How much income is http://scholar.google.com/scholar_lookup?title=The+endocytic+pathway+mediates+cell+entry+of+dsRNA+to+induce+RNAi+silencing&author=MC+Saleh&author=RP+van+Rij&author=A+Hekele&author=A+Gillis&author=E+Foley&journal=Nat.+Cell+Biol.&volume=8&pages=793-802&publication_year=2006& earning monthly?
- How much profit does http://scholar.google.com/scholar_lookup?title=The+next+generation+of+insecticides%3A+dsRNA+is+stable+as+a+foliar-applied+insecticide&author=K+San+Miguel&author=JG+Scott&journal=Pest+Manag.+Sci.&volume=72&pages=801-9&publication_year=2016& make?
- Monthly income for http://scholar.google.com/scholar_lookup?title=Comparison+of+silencing+efficacy+of+the+antenna-rich+genes+by+different+dsRNA+delivery+methods+in+Locusta+migratoria&author=X+Shi&author=Y+Zhang&author=KY+Zhu&author=E+Ma&author=J+Zhang&journal=Chin.+J.+Appl.+Entomol&volume=54&pages=780-90&publication_year=2017&
- How much does http://scholar.google.com/scholar_lookup?title=SID-1+is+a+dsRNA-selective+dsRNA-gated+channel&author=JD+Shih&author=CP.+Hunter&journal=RNA&volume=17&pages=1057-65&publication_year=2011& earn?
- How much revenue does http://scholar.google.com/scholar_lookup?title=Reduced+stability+and+intracellular+transport+of+dsRNA+contribute+to+poor+RNAi+response+in+lepidopteran+insects&author=JN+Shukla&author=M+Kalsi&author=A+Sethi&author=KE+Narva&author=E+Fishilevich&journal=RNA+Biol&volume=13&pages=656-69&publication_year=2016& produce monthly?
- How much income does http://scholar.google.com/scholar_lookup?title=Comparative+analysis+of+double-stranded+RNA+degradation+and+processing+in+insects&author=IK+Singh&author=S+Singh&author=K+Mogilicherla&author=JN+Shukla&author=SR+Palli&journal=Sci.+Rep.&volume=7&pages=17059&publication_year=2017& have?
- See how much http://scholar.google.com/scholar_lookup?title=PIWI-interacting+small+RNAs%3A+the+vanguard+of+genome+defence&author=MC+Siomi&author=K+Sato&author=D+Pezic&author=AA+Aravin&journal=Nat.+Rev.+Mol.+Cell+Biol.&volume=12&pages=246-58&publication_year=2011& makes per month
- http://scholar.google.com/scholar_lookup?title=Contributions+of+dsRNases+to+differential+RNAi+efficiencies+between+the+injection+and+oral+delivery+of+dsRNA+in+Locusta+migratoria&author=H+Song&author=Y+Fan&author=J+Zhang&author=AM+Cooper&author=K+Silver&journal=Pest+Manag.+Sci&volume=75&pages=1707-17&publication_year=2019&'s financial summary
- How much does http://scholar.google.com/scholar_lookup?title=A+double-stranded+RNA+degrading+enzyme+reduces+the+efficiency+of+oral+RNA+interference+in+migratory+locust&author=HF+Song&author=JQ+Zhang&author=DQ+Li&author=AMW+Cooper&author=K+Silver&journal=Insect+Biochem.+Mol.+Biol.&volume=86&pages=68-80&publication_year=2017& pull in monthly?
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- Get to know http://scholar.google.com/scholar_lookup?title=RNAi-based+reverse+genetics+in+the+chelicerate+model+Tetranychus+urticae%3A+a+comparative+analysis+of+five+methods+for+gene+silencing&author=T+Suzuki&author=MA+Nunes&author=MU+Espana&author=HH+Namin&author=PY+Jin&journal=PLOS+ONE&volume=12&pages=e0180654&publication_year=2017&'s earnings
- What's the financial intake of http://scholar.google.com/scholar_lookup?title=The+evolutionary+journey+of+Argonaute+proteins&author=DC+Swarts&author=K+Makarova&author=Y+Wang&author=K+Nakanishi&author=RF+Ketting&journal=Nat.+Struct.+Mol.+Biol.&volume=21&pages=743-53&publication_year=2014&?
- How much profit does http://scholar.google.com/scholar_lookup?title=The+possible+impact+of+persistent+virus+infection+on+the+function+of+the+RNAi+machinery+in+insects%3A+a+hypothesis&author=L+Swevers&author=JV+Broeck&author=G+Smagghe&journal=Front.+Physiol.&volume=4&pages=319&publication_year=2013& generate?
- How much revenue does http://scholar.google.com/scholar_lookup?title=Defense+mechanisms+against+viral+infection+in+Drosophila%3A+RNAi+and+non-RNAi&author=L+Swevers&author=J+Liu&author=G+Smagghe&journal=Viruses&volume=10&pages=E230&publication_year=2018& produce monthly?
- How much does http://scholar.google.com/scholar_lookup?title=Oral+RNAi+to+control+Drosophila+suzukii%3A+laboratory+testing+against+larval+and+adult+stages&author=CNT+Taning&author=O+Christiaens&author=N+Berkvens&author=H+Casteels&author=M+Maes&author=G+Smagghe&journal=J.+Pest+Sci.&volume=89&pages=803-14&publication_year=2016& gross monthly?
- How much does http://scholar.google.com/scholar_lookup?title=Engineered+flock+house+virus+for+targeted+gene+suppression+through+RNAi+in+fruit+flies+%28Drosophila+melanogaster%29+in+vitro+and+in+vivo&author=CNT+Taning&author=O+Christiaens&author=XX+Li&author=L+Swevers&author=H+Casteels&journal=Front.+Physiol.&volume=9&pages=805&publication_year=2018& generate monthly?
- How much does http://scholar.google.com/scholar_lookup?title=Circulating+immune+cells+mediate+a+systemic+RNAi-based+adaptive+antiviral+response+in+Drosophila&author=M+Tassetto&author=M+Kunitomi&author=R+Andino&journal=Cell&volume=169&pages=314-25&publication_year=2017& generate monthly?
- How much does http://scholar.google.com/scholar_lookup?title=RNA+interference+in+Lepidoptera%3A+an+overview+of+successful+and+unsuccessful+studies+and+implications+for+experimental+design&author=O+Terenius&author=A+Papanicolaou&author=JS+Garbutt&author=I+Eleftherianos&author=H+Huvenne&journal=J.+Insect+Physiol.&volume=57&pages=231-45&publication_year=2011& make?
- What's the monthly income of http://scholar.google.com/scholar_lookup?title=Enhanced+whitefly+resistance+in+transgenic+tobacco+plants+expressing+double+stranded+RNA+of+v-ATPase+A+gene&author=N+Thakur&author=SK+Upadhyay&author=PC+Verma&author=K+Chandrashekar&author=R+Tuli&author=PK+Singh&journal=PLOS+ONE&volume=9&pages=e87235&publication_year=2014&?
- What's http://scholar.google.com/scholar_lookup?title=Developmental+control+of+a+lepidopteran+pest+Spodoptera+exigua+by+ingestion+of+bacteria+expressing+dsRNA+of+a+non-midgut+gene&author=HG+Tian&author=H+Peng&author=Q+Yao&author=HX+Chen&author=Q+Xie&journal=PLOS+ONE&volume=4&pages=e6225&publication_year=2009&'s gross income?
- What's the revenue for http://scholar.google.com/scholar_lookup?title=Exploring+systemic+RNA+interference+in+insects%3A+a+genome-wide+survey+for+RNAi+genes+in+Tribolium&author=Y+Tomoyasu&author=SC+Miller&author=S+Tomita&author=M+Schoppmeier&author=D+Grossmann&author=G+Bucher&journal=Genome+Biol&volume=9&pages=R10&publication_year=2008&?
- What is the earnings of http://scholar.google.com/scholar_lookup?title=Dual-layer+transposon+repression+in+heads+of+Drosophila+melanogaster&author=M+Van+Den+Beek&author=B+Da+Silva&author=J+Pouch&author=MEA+Chaouche&author=C+Carre&author=C+Antoniewski&journal=RNA&volume=24&pages=1749-60&publication_year=2018&?
- http://scholar.google.com/scholar_lookup?title=Characterization+of+Argonaute+family+members+in+the+silkworm%2C+Bombyx+mori&author=G+Wang&author=L+Jiang&author=L+Zhu&author=T+Cheng&author=W+Niu&journal=Insect+Sci.&volume=20&pages=78-91&publication_year=2013&'s total income per month
- Profit of http://scholar.google.com/scholar_lookup?title=Variation+in+RNAi+efficacy+among+insect+species+is+attributable+to+dsRNA+degradation+in+vivo&author=K+Wang&author=Y+Peng&author=J+Pu&author=W+Fu&author=J+Wang&author=Z+Han&journal=Insect+Biochem.+Mol.+Biol.&volume=77&pages=1-9&publication_year=2016&
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- How profitable is http://scholar.google.com/scholar_lookup?title=Gene+silencing+in+non-model+insects%3A+overcoming+hurdles+using+symbiotic+bacteria+for+trauma-free+sustainable+delivery+of+RNA+interference&author=M+Whitten&author=P.+Dyson&journal=Bioessays&volume=39&issue=3&pages=1600247&publication_year=2017&?
- How much income does http://scholar.google.com/scholar_lookup?title=Ingested+double-stranded+RNAs+can+act+as+species-specific+insecticides&author=S+Whyard&author=AD+Singh&author=S+Wong&journal=Insect+Biochem.+Mol.+Biol.&volume=39&pages=824-32&publication_year=2009& have?
- How much does http://scholar.google.com/scholar_lookup?title=Systemic+RNAi+in+C.+elegans+requires+the+putative+transmembrane+protein+SID-1&author=WM+Winston&author=C+Molodowitch&author=CP+Hunter&journal=Science&volume=295&pages=2456-59&publication_year=2002& earn?
- How much does http://scholar.google.com/scholar_lookup?title=Caenorhabditis+elegans+SID-2+is+required+for+environmental+RNA+interference&author=WM+Winston&author=M+Sutherlin&author=AJ+Wright&author=EH+Feinberg&author=CP+Hunter&journal=PNAS&volume=104&pages=10565-70&publication_year=2007& generate monthly?
- See how much http://scholar.google.com/scholar_lookup?title=Gene+silencing+in+insect+cells+using+RNAi&author=H+Wu&author=J+March&author=W+Bentley&journal=Methods+Mol.+Biol.&volume=1350&pages=469-76&publication_year=2016& makes per month
- How much income is http://scholar.google.com/scholar_lookup?title=Scavenger+receptor-mediated+endocytosis+facilitates+RNA+interference+in+the+desert+locust%2C+Schistocerca+gregaria&author=N+Wynant&author=D+Santos&author=P+Van+Wielendaele&author=J+Vanden+Broeck&journal=Insect+Mol.+Biol.&volume=23&pages=320-29&publication_year=2014& earning monthly?
- Discover the revenue of http://scholar.google.com/scholar_lookup?title=Biological+mechanisms+determining+the+success+of+RNA+interference+in+insects&author=N+Wynant&author=D+Santos&author=J+Vanden+Broeck&journal=Int.+Rev.+Cell+Mol.+Biol.&volume=312&pages=139-67&publication_year=2014&
- Get to know what's the income of http://scholar.google.com/scholar_lookup?title=Identification%2C+functional+characterization+and+phylogenetic+analysis+of+double+stranded+RNA+degrading+enzymes+present+in+the+gut+of+the+desert+locust%2C+Schistocerca+gregaria&author=N+Wynant&author=D+Santos&author=R+Verdonck&author=J+Spit&author=P+Van+Wielendaele&author=J+Vanden+Broeck&journal=.+Insect+Biochem.+Mol.+Biol.&volume=46&pages=1-8&publication_year=2014&
- http://scholar.google.com/scholar_lookup?title=Tissue-dependence+and+sensitivity+of+the+systemic+RNA+interference+response+in+the+desert+locust%2C+Schistocerca+gregaria&author=N+Wynant&author=H+Verlinden&author=B+Breugelmans&author=G+Simonet&author=J+Vanden+Broeck&journal=Insect+Biochem.+Mol.+Biol.&volume=42&pages=911-17&publication_year=2012&'s financial summary
- How much revenue does http://scholar.google.com/scholar_lookup?title=Clathrin-dependent+endocytosis+plays+a+predominant+role+in+cellular+uptake+of+double-stranded+RNA+in+the+red+flour+beetle&author=D+Xiao&author=XW+Gao&author=JP+Xu&author=X+Liang&author=QQ+Li&journal=Insect+Biochem.+Mol.+Biol.&volume=60&pages=68-77&publication_year=2015& bring in?
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- Find out how much http://scholar.google.com/scholar_lookup?title=MicroRNA-133+inhibits+behavioral+aggregation+by+controlling+dopamine+synthesis+in+locusts&author=ML+Yang&author=YY+Wei&author=F+Jiang&author=YL+Wang&author=XJ+Guo&journal=PLOS+Genet&volume=10&pages=e1004206&publication_year=2014& earns monthly
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