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Title[redir]:
Transplantation
Description:
urified human B cells devoid of T cells were stimulated with CD40L expressing L cells, or by anti-CD40 mAb with or without Toll-like receptor triggering, all in the presence of B-cell activating cytokines. These three protocols resulted in various degrees of B-cell stimulation. We added four commonly used immunosuppressive drugs (tacrolimus, cyclosporin, mycophenolic acid [MPA], and rapamycin) to these cultures and tested a variety of parameters of B-cell activity including proliferation, apoptosis induction, and both IgM and IgG production. Results. Tacrolimus and cyclosporin marginally inhibited B-cell proliferation and immunoglobulin production, and the extent of inhibition depended on the degree of the B-cell stimulation. In contrast, MPA and rapamycin profoundly inhibited both B-cell proliferation and immunoglobulin production, which was independent of the degree of B-cell stimulation. Both drugs induced B-cell apoptosis. Moreover, rapamycin caused a reduction in the number of B cells capable of producing immunoglobulins. Conclusions. Our data show that MPA and rapamycin are capable of strongly inhibiting B cells responses. This provides a rationale for the use of both MPA and rapamycin to prevent or counteract humoral responses....
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Keywords {🔍}
cells, drugs, immunosuppressive, rapamycin, effects, proliferation, production, bcell, immunoglobulin, mpa, anticd, igg, concentrations, igm, effect, apoptosis, producing, data, cyclosporin, ngml, humoral, cell, lcdl, fig, shown, rejection, stimulated, tacrolimus, number, netherlands, culture, calcineurin, cultured, mabcpg, added, inhibitors, mmf, presence, inhibition, plates, percentage, transplantation, tested, inhibiting, immunosuppressants, antibodies, performed, stimulus, observed, elispot,
Topics {✒️}
cd103+cd8+ regulatory t-cells biotin-labeled goat anti-igm anti-cd40 mab+cpg resulted lacking b-cell-specific drugs anti-cd40 mab+cpg-stimulated anti-cd40 mab+cpg stimulated anti-cd40 mab+cpg stimulus antigen-specific t-cell b-cell-specific agent regulatory t-cell subsets b-cell activating cytokines rapamycin-induced b-cell apoptosis b-cell receptor signaling anti-cd40 mab+cpg detach-a-bead cd19 streptavidin-conjugated alkaline phosphatase low b-cell proliferation cd4+cd25+foxp3+ regulatory anti-cd40 mab resulted ongoing b-cell response solid-organ transplant recipients anti-cd40 mab-stimulated treat antibody-mediated rejections computer-guided elispot reader citric acid/pbs buffer mismatched donor antigens antigen-specific interactions b-cell responses low cell yields low cell yield open bars represent anti-cd40 mab anti-cd40+cpg b-cell parameters identify specific populations cd4+cd25+foxp3+ conjugated cd19 mab protective antibody titers influenza-specific antibodies journal authors submit counteract humoral responses anti-cd40-stimulated b-cell apoptosis b-cell proliferation b-cell cultures antigen presenting function round bottom plate view full text fixed cell concentrations b-cell stimulation
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