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Title[redir]:
Transplantation
Description:
ell death, or induction of anergy. One of the potential mechanisms in the immunosuppressive accomplishment of HLA-G-ILT2 interactions involves the expansion of myeloid-derived suppressor cells (MDSCs). The potential of MDSCs in transplantation has not yet been exploited. Methods. (1) Detailed phenotypic characteristics, immunosuppressive potential of MDSCs expanded by means of inhibitory receptor ILT2 and its ligands, and allogeneic transplant-activated MDSCs were obtained in mice. (2) Oligo- and real-time pathway-specific polymerase chain reaction arrays were performed to characterize ILT2-specific MDSCs. (3) Skin allograft survival after adoptive transfer of MDSCs was studied. Results. Engagement of ILT2 receptors, especially by HLA-G, expanded the population of MDSCs with enhanced suppressive activity. Adoptive transfer of MDSCs generated by ILT2 receptor and its ligands prolonged graft survival in recipients of allogeneic skin transplant. We have proposed pathways for enhancement of immunosuppressive activities and expansion of MDSCs by ILT2 and HLA-G. Conclusions. Our results suggest that induction of MDSCs using ILT2 inhibitory receptor/HLA-G ligand may be an attractive strategy for preventing rejection of immunogenic organs or tissues in clinical transplantation....
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Keywords {π}
mice, mdscs, cells, ilt, skin, cdbgr, control, hlag, cbl, allogeneic, expression, transplantation, potential, genes, fig, receptor, tcell, expansion, allograft, data, inhibitory, survival, ligand, cell, compared, suppressive, results, immune, mechanisms, immunosuppressive, performed, receptors, activity, isolated, analysis, expressed, tolerance, unique, production, total, increased, graft, important, proliferation, wildtype, activated, activation, induction, transgenic, determined,
Topics {βοΈ}
hla-g-ilt2 interactions involves promote long-term survival tgf-Ξ²-induced transcript 4 ge healthcare bio-sciences mhc class ii-positive t-cell receptor complex Ξ²2-microglobulin knockout background myeloid-derived suppressor cells c-type mannose receptor pcr array rt2 hla-g-coated beads cd11b+/gr-1+ cells isolated allophycocyanin-conjugated mabs specific t-cell proliferation assay tumor-bearing mice differed modulates t-cell function inhibits t-cell proliferation cd3 ΞΆ chain inhibit interferon-Ξ³ production mhc class ii rna stat-60tm kit long-term survival cells suppress alloantigen-activated pe-conjugated anti-cd11b fitc-conjugated anti-gr-1 inhibitory receptor-activated mdscs ligand-free ilt2 mice cytokine/receptor pathways play murine ligand h-2db ilt2 inhibitory receptor/hla characterize ilt2-specific mdscs human leukocyte antigen key enzymes induced bm12 allograft-activated ilt2 standard 51cr-release assay inhibitory receptors-activated mdscs blot imaging system myeloid-derived cells ilt2 mice induced tumor-bearing mice mdscs allogeneic transplant-activated mdscs ifn-Ξ³ elisa kits cd11b+/gr-1+ cell allogeneic skin grafts mouse angiogenesis array skin graft recipients cell-free supernatants bm12 skin grafts allogeneic skin transplant i-abm12 alloantigen
Questions {β}
- HLA-G in transplantation: A relevant molecule for inhibition of graft rejection?
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