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Title[redir]:
The American Journal of Surgical Pathology
Description:
re specific indications for use of immunohistochemistry for the somatostatin receptor subtype 2A, but this has up to now been limited by the lack of an adequate reliable antibody. The aim of this study was to correlate immunohistochemistry using the new monoclonal anti-somatostatin receptor subtype 2A antibody UMB-1 with the gold standard in vitro method quantifying somatostatin receptor levels in tumor tissues. A UMB-1 immunohistochemistry protocol was developed, and tumoral UMB-1 staining levels were compared with somatostatin receptor binding site levels quantified with in vitro 125I-[Tyr3]-octreotide autoradiography in 89 tumors. This allowed defining an immunohistochemical staining threshold permitting to distinguish tumors with somatostatin receptor levels high enough for clinical applications from those with low receptor expression. The presence of >10% positive tumor cells correctly predicted high receptor levels in 95% of cases. In contrast, absence of UMB-1 staining truly reflected low or undetectable somatostatin receptor expression in 96% of tumors. If 1% to 10% of tumor cells were stained, a weak staining intensity was suggestive of low somatostatin receptor levels. This study allows for the first time a reliable recommendation for eligibility of an individual patient for in vivo somatostatin receptor targeting based on somatostatin receptor immunohistochemistry. Under optimal methodological conditions, UMB-1 immunohistochemistry may be equivalent to in vitro receptor autoradiography....
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Keywords {🔍}
receptor, somatostatin, journal, immunohistochemistry, levels, tumors, umb, issue, tumor, staining, access, subscribe, alerts, articles, authors, abstract, subtype, antibody, vivo, targeting, high, expression, vitro, low, log, register, logo, issues, monoclonal, suitable, buy, reliable, study, autoradiography, cells, individual, text, subscribers, request, browse, content, submit, service, privacy, policy, secondary, advanced, search, info, previous,
Topics {✒️}
vitro 125i-[tyr3]-octreotide autoradiography vitro receptor autoradiography somatostatin receptor expression somatostatin receptor immunohistochemistry low receptor expression adequate reliable antibody radiolabeled somatostatin analogues journal authors submit full text access umb-1 immunohistochemistry protocol weak staining intensity optimal methodological conditions artificial intelligence training tumor cells etoc alerts service request 800-638-3030 umb-1 staining register subscribe umb-1 immunohistochemistry somatostatin receptors american journal journal tables individual subscribers log issue tumor tissues correlate immunohistochemistry meike md aurel md reflected low website subscribe journal reliable recommendation individual patient center submit neuroendocrine tumors distinguish tumors agnes phd† surgical pathology 36 specific indications gold standard allowed defining clinical applications email inbox rights reserved data mining tumor immunohistochemistry suitable authors content
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