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Title[redir]:
Melanoma Research
Description:
compounds that preferentially decreased proliferation in multiple melanoma subtypes compared with benign melanocytes. DSF, a member of the dithiocarbamate family, is a copper (Cu) chelator, and Cu has been shown previously to enhance DSF-mediated growth inhibition and apoptosis in cancer cells. Here, we report that in the presence of free Cu, DSF inhibits cellular proliferation and induces apoptosis in a panel of cell lines representing primary and metastatic nodular and superficial spreading melanoma. Both decreased cellular proliferation and increased apoptosis were seen at 50–500 nmol/l DSF concentrations that are achievable through oral dosing of the medication. In the presence of Cu, DSF caused activation of the extrinsic pathway of apoptosis as measured by caspase-8 cleavage. The addition of Z-IETD-FMK, a selective caspase-8 inhibitor, was protective against DSF–Cu-induced apoptosis. Production of reactive oxygen species (ROS) in response to DSF–Cu treatment preceded the induction of apoptosis. Both ROS production and apoptosis were prevented by coincubation of N-acetyl cysteine, a free radical scavenger. Our study shows that DSF might be used to target both nodular and superficial spreading melanoma through ROS production and activation of the extrinsic pathway of apoptosis....
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Keywords {🔍}
journal, apoptosis, melanoma, dsf, issue, access, subscribe, alerts, submit, articles, authors, abstract, activation, extrinsic, pathway, proliferation, production, ros, log, register, issues, research, disulfiram, induces, reactive, oxygen, species, buy, cancer, current, response, decreased, presence, free, cellular, nodular, superficial, spreading, caspase, text, subscribers, request, browse, content, service, privacy, policy, manuscript, logo, advanced,
Topics {✒️}
dsf–cu treatment preceded dsf–cu-induced apoptosis journal authors submit decreased cellular proliferation full text access preferentially decreased proliferation superficial spreading melanoma reactive oxygen species z-ietd-fmk n-acetyl cysteine artificial intelligence training selective caspase-8 inhibitor free radical scavenger extrinsic apoptotic pathway service request 800-638-3030 dsf caused activation register subscribe individual subscribers log journal tables induces apoptosis current standard melanoma morrison etoc alerts website subscribe dsf concentrations extrinsic pathway free cu center submit journal caspase-8 cleavage deadly form skin cancer nontoxic therapies disulfiram drug screen benign melanocytes dithiocarbamate family copper shown previously cancer cells oral dosing study shows email inbox rights reserved data mining issue increased apoptosis metastatic nodular ros production authors
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