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Title[redir]:
Anti-Cancer Drugs
Description:
emained untreated. Tumor cell growth, cell cycle progression, and cell cycle-regulating proteins were investigated by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay, flow cytometry, and western blotting, respectively. Effects of VPA on histone H3 and H4 acetylation and HDAC3 and HDAC4 were also determined. Without preincubation, no tumor cell growth reduction was observed with 0.125 and 0.25 mmol/l VPA in TCCSUP, UMUC-3, and RT-112 cells, whereas 0.5 and 1 mmol/l VPA diminished the cell number significantly. VPA (0.25 mmol/l) did exert tumor growth-blocking effects after a 3-day preincubation. To achieve antitumor effects with VPA (0.125 mmol/l), a 5-day preincubation was necessary. A 3-day or 5-day preincubation was also necessary to distinctly delay cell cycle progression, with maximum effects at VPA (1 mmol/l). After the 5-day preincubation, the cell cycle-regulating proteins cdk1, cdk2, cdk4, and cyclins B, D1, and E were reduced, whereas p27 was enhanced. Diminished HDAC3 and 4 expression induced by VPA was accompanied by elevated acetylation of H3 and H4. VPA exerted growth-blocking properties on a panel of bladder cancer cell lines, commensurate with dose and exposure time. Long-term application induced much stronger effects than did shorter application and should be considered when designing therapeutic strategies for treating bladder carcinoma....
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Keywords {🔍}
cell, vpa, journal, cancer, mmoll, preincubation, bladder, effects, day, access, issue, hdac, subscribe, alerts, submit, authors, abstract, cycle, progression, cells, growth, tumor, cdk, log, register, articles, advanced, issues, vitro, buy, histone, tccsup, umuc, cycleregulating, proteins, acetylation, diminished, growthblocking, induced, application, text, subscribers, request, article, drug, browse, content, service, privacy, policy,
Topics {✒️}
cell cycle-regulating proteins cell cycle progression long-term application induced 0b013e32834a2c70 buy abstract tumor cell growth cell number significantly small cell lung journal authors submit treating bladder carcinoma full text access inhibitor valproic acid controls remained untreated 5-diphenyltetrazolium bromide assay designing therapeutic strategies read wound healing p53 mutants mechanism artificial intelligence training achieve antitumor effects service request 800-638-3030 register subscribe individual subscribers log journal tables drug delivery rt-112 cells etoc alerts hdac 4 expression induced shorter application website subscribe center submit maximum effects stronger effects journal advanced histone deacetylase 5-dimethylthiazol-2-yl flow cytometry western blotting elevated acetylation exposure time catheter thrombosis maintenance therapy checkpoint modulator synthetic lethality cancer diagnostics back email inbox rights reserved data mining preincubation periods
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