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Applied Immunohistochemistry & Molecular Morphology
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sections. Twelve of these 48 tumors had a difference of at least 2 arbitrary units in the in situ compared with the invasive part of the tumor with regard to the HER-2/neu status analyzed by HercepTest (immunohistochemistry). Eight of these 12 tumors were reanalyzed with fluorescent in situ hybridization and immunohistochemistry with and without a new Automated Cellular Imaging System. In this limited material, immunohistochemistry in combination with the Automated Cellular Imaging System seemed to have a better correlation with fluorescent in situ hybridization than immunostaining analyzed manually. In conclusion, HER-2/neu expression is not seldom heterogeneous in invasive compared with in situ components within a tumor. This finding should be considered in the choice of evaluation method. To avoid heterogeneity as a confounding factor in HER-2/neu analyses, detection methods such as immunohistochemistry and fluorescent in situ hybridization, which can provide evaluation in a preserved tissue architecture, should be used. Perhaps the intratumor HER-2/neu heterogeneity can explain some of the unexpected failures of trastuzumab therapy....
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Keywords {🔍}
journal, situ, herneu, immunohistochemistry, invasive, heterogeneity, issue, fluorescent, hybridization, tumors, access, subscribe, alerts, articles, authors, abstract, evaluation, intratumor, components, phd, analyzed, log, register, issues, primary, breast, buy, tissue, compared, tumor, automated, cellular, imaging, system, text, subscribers, request, browse, content, submit, service, privacy, policy, logo, advanced, search, info, previous, research, cerbb,
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primary breast carcinoma journal authors submit full text access analyzed tissue sections immunostaining analyzed manually preserved tissue architecture artificial intelligence training 2/neu status analyzed service request 800-638-3030 etoc alerts register subscribe individual subscribers log buy abstract journal tables issue website subscribe invasive components journal invasive component invasive part invasive compared center submit situ components c-erbb-2 situ hybridization special focus jenny md barbro md jonas md goran md 2 arbitrary units situ compared limited material 2/neu expression seldom heterogeneous confounding factor 2/neu analyses detection methods unexpected failures trastuzumab therapy email inbox rights reserved data mining 2/neu heterogeneity avoid heterogeneity evaluation method provide evaluation intratumor heterogeneity phd authors
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