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Title[redir]:
Anti-Cancer Drugs
Description:
cells. Furthermore, all-trans retinoic acid (ATRA) augmented the anticancer effects of CQ and HCQ as evidenced by significant reductions in Ki67-positive cancer cells and clonogenicity compared with cells treated with CQ or HCQ in the absence of ATRA. As an earlier study suggested that CQ, HCQ, and ATRA are breast cancer cell differentiation agents, these agents were screened in cell-free histone deacetylase (HDAC) and histone acetyltransferase (HAT) assays. ATRA, but not CQ or HCQ, inhibited HDAC activity in HeLa nuclear extracts. Growth inhibitory concentrations of HCQ and ATRA stimulated purified p300/CBP-associated factor, where CBP is the cAMP-response element binding protein, HAT activity. To investigate whether growth inhibitory concentrations of these agents influenced protein acetylation in cells, gel-purified histone H3 and histone H4 were analyzed using mass spectrometry. HCQ alone and HCQ+ATRA treatments altered the acetylation status in the N-terminal lysines of histones H3 and H4 compared with dimethyl sulfoxide (DMSO) controls. The results indicated that HCQ and ATRA regulate protein acetylation events in MCF-7 breast cancer cells, and identify a potential mechanism for their effects on breast cancer cell growth and differentiation....
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Keywords {🔍}
hcq, journal, cells, cancer, breast, atra, growth, histone, issue, acetylation, access, subscribe, alerts, submit, authors, abstract, agents, protein, log, register, articles, issues, hydroxychloroquine, chloroquine, alltrans, retinoic, acid, regulate, anticancer, drugs, buy, potential, mcf, inhibited, effects, compared, cell, differentiation, hdac, activity, inhibitory, concentrations, text, subscribers, request, browse, content, service, privacy, policy,
Topics {✒️}
ki67-positive cancer cells cell-free histone deacetylase gel-purified histone h3 trans retinoic acid hcq+atra treatments altered 0b013e32832f4e50 buy abstract journal authors submit full text access growth inhibitory concentrations antimalarial drugs chloroquine earlier study suggested hela nuclear extracts n-terminal lysines artificial intelligence training service request 800-638-3030 register subscribe individual subscribers log inhibited hdac activity journal tables cancer treatment histone acetylation cells treated etoc alerts acetylation status histones h3 website subscribe histone acetyltransferase histone h4 center submit journal potential applications significant reductions clonogenicity compared hat activity mass spectrometry h4 compared dimethyl sulfoxide potential mechanism email inbox rights reserved data mining issue mcf-7 cells anticancer effects agents growth cbp differentiation authors
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