
DOI . ORG {
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Title[redir]:
NANOS3 function in human germ cell development | Human Molecular Genetics | Oxford Academic
Description:
Abstract. Human infertility is common and frequently linked to poor germ cell development. Yet, human germ cell development is poorly understood, at least
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Keywords {π}
nanos, germ, cells, cell, expression, human, development, google, vol, scholar, worldcat, fig, protein, genes, crossrefpubmed, hescs, differentiation, gene, min, decreased, expressed, levels, vasa, analysis, days, required, proteins, detected, supplementary, dazl, adult, control, testis, material, differentiated, antibody, mouse, stella, antibodies, drosophila, homologs, fetal, western, blot, undifferentiated, species, tissue, line, pumilio, mrna,
Topics {βοΈ}
oxford university press mathematics books journals university press partners org/journal/2007/08/quantifying-western-blots neighboring extra-embryonic ectoderm contact extra-embryonic ectoderm anti-chicken horseradish peroxidase negative-feedback loop exists pentr/h1/t0 vector 5% tris-borate-edta gels interacting rna-binding proteins low-adhesion culture dish real-time quantitative pcr cchc metal-binding domain meiotic mis-segregation occurs germ cell-specific markers rna-binding protein gene germ cell-specific proteins hesc-derived germs cells gene-specific taqman probes hesc-derived germ cells heart-specific cell types pre-implantation mouse epiblast rna-binding protein nanos2 pre-immune anti-serum germ plasm-localized rna access accessibility contact article sign gfp-tagged nanos3 protein human testis cross-sections volume 20 1 june 2011 mis-segregation occurs discussion discovery materials marketing citing articles human stem cell google scholar latest permissions vitro-derived germ cells germ stem cell germ cell numbers somatic cell fate single nanos homolog rna-binding proteins specific cell types extra-embryonic cluster germ cell fate closely related mammals
Schema {πΊοΈ}
ScholarlyArticle:
context:https://schema.org
id:https://academic.oup.com/hmg/article/20/11/2238/635831
name:NANOS3 function in human germ cell development
about:
genes
germ cells
rna, messenger
datePublished:2011-03-19
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type:PublicationIssue
issueNumber:11
datePublished:2011-06-01
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type:Periodical
name:Human Molecular Genetics
issn:
1460-2083
url:https://dx.doi.org/10.1093/hmg/ddr114
keywords:
genes
germ cells
rna, messenger
inLanguage:en
copyrightHolder:Oxford University Press
copyrightYear:2025
publisher:Oxford University Press
author:
name:Julaton, Vanessa T. Angeles
affiliation:Institute for Stem Cell Biology and Regenerative Medicine, Department of Obstetrics and Gynecology , Stanford University School of Medicine, Stanford University , Palo Alto, CA 94305 , USA
type:Person
name:Reijo Pera, Renee A.
affiliation:Institute for Stem Cell Biology and Regenerative Medicine, Department of Obstetrics and Gynecology , Stanford University School of Medicine, Stanford University , Palo Alto, CA 94305 , USA
type:Person
description:Abstract. Human infertility is common and frequently linked to poor germ cell development. Yet, human germ cell development is poorly understood, at least
pageStart:2238
pageEnd:2250
siteName:OUP Academic
thumbnailURL:https://oup.silverchair-cdn.com/oup/backfile/Content_public/Journal/hmg/20/11/10.1093_hmg_ddr114/2/m_ddr11401.jpeg?Expires=1813887874&Signature=svctShskXO-7U8hxAh1BUaZ~4apcOE8Y7MaKuGyRpVoX31obVXLvAMsP~~rU646WCSNHyYk~uWcjO-SSCBcnREZSRL-Dhxk7BBUrxhGUxvd~ibWht972BBUWphsoubIxscfbpm-IZMj0AXSF-huNvK7kSDyVXE2Y11saOQDYFlE4sSOaS9gS2YhDTQfh2PvIcAA7x86YpVU-Msefr7KpT2w77KLiImw1FhCmczF-KSD1rzRp1AOiEKA-ORF-ikIP~eFDbinpqsLscSvQT95pLAVn9rfpLLsl~s1do1q9Uhzll6kXso2mdbdT~82c570bmaXrGrOk4JdJ7m8S4aCo0Q__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA
headline:NANOS3 function in human germ cell development
image:https://oup.silverchair-cdn.com/oup/backfile/Content_public/Journal/hmg/20/11/10.1093_hmg_ddr114/2/m_ddr11401.jpeg?Expires=1813887874&Signature=svctShskXO-7U8hxAh1BUaZ~4apcOE8Y7MaKuGyRpVoX31obVXLvAMsP~~rU646WCSNHyYk~uWcjO-SSCBcnREZSRL-Dhxk7BBUrxhGUxvd~ibWht972BBUWphsoubIxscfbpm-IZMj0AXSF-huNvK7kSDyVXE2Y11saOQDYFlE4sSOaS9gS2YhDTQfh2PvIcAA7x86YpVU-Msefr7KpT2w77KLiImw1FhCmczF-KSD1rzRp1AOiEKA-ORF-ikIP~eFDbinpqsLscSvQT95pLAVn9rfpLLsl~s1do1q9Uhzll6kXso2mdbdT~82c570bmaXrGrOk4JdJ7m8S4aCo0Q__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA
image:alt:NANOS is conserved in humans, with enriched expression in human gonadal tissue. (A) Alignment of the protein sequences of NANOS homologs in multiple organisms reveals conservation of the NANOS genes from invertebrates to vertebrates. Mammalian species (M. musculus/mouse, R. norvegicus/rat, H. sapiens/human) each have three NANOS homologs and share the highest sequence similarity, relative to more primitive species (X. laevis/frog, B. mori/silkworm, D. rerio/zebrafish, C. elegans/worm, D. melanogaster/fly). (B) The NANOS protein sequence is mostly divergent outside of the eight amino acids which comprise two zinc finger motifs (CCHC CCHC), shown in red. (C) Quantitative PCR of NANOS2, NANOS3, DAZL and VASA in fetal and adult human tissue cDNA. NANOS2 and 3 expression are highest in the fetal and adult testis, and are likewise enriched in the fetal and adult ovary. Gene expression is normalized to GAPDH, RPLPO and EEF1A1. (D) Characterization of NANOS expression in adult human testis lysates via western blot analysis with custom NANOS antibodies. NANOS1 is detected at βΌ60 kDa. NANOS2 is detected at βΌ24kDa. NANOS3 is detected at βΌ28kDa. (E) NANOS expression in human gonadal tissue is determined via western blot analysis of human tissue lysates. NANOS1, 2 and 3 are expressed in the fetal and adult ovaries and testes; NANOS3 is also expressed in the adult brain. GAPDH is included as a loading control.
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Person:
name:Julaton, Vanessa T. Angeles
affiliation:Institute for Stem Cell Biology and Regenerative Medicine, Department of Obstetrics and Gynecology , Stanford University School of Medicine, Stanford University , Palo Alto, CA 94305 , USA
name:Reijo Pera, Renee A.
affiliation:Institute for Stem Cell Biology and Regenerative Medicine, Department of Obstetrics and Gynecology , Stanford University School of Medicine, Stanford University , Palo Alto, CA 94305 , USA
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