DOI . ORG {
}
We began analyzing https://pnas.org/doi/full/10.1073/pnas.94.4.1124, but it redirected us to https://pnas.org/doi/full/10.1073/pnas.94.4.1124. The analysis below is for the second page.
Title[redir]:
Antifolate-resistant mutants of Plasmodium falciparum dihydrofolate reductase | PNAS
Description:
Single and multiple mutations at residues 16, 51, 59, 108, and 164 of Plasmodium falciparum dihydrofolate reductase (pfDHFR) have been linked to antifolate resistance in malaria. We prepared and ch...
Title[redir]:
Antifolate-resistant mutants of Plasmodium falciparum dihydrofolate reductase | PNAS
Description:
Single and multiple mutations at residues 16, 51, 59, 108, and 164 of Plasmodium falciparum dihydrofolate reductase (pfDHFR) have been linked to antifolate resistance in malaria. We prepared and ch...
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Keywords {🔍}
mutants, mutant, pyr, resistance, cyc, dhfr, single, values, mutations, mutation, wildtype, fold, resistant, google, scholar, enzyme, crossref, nature, double, pubmed, multiple, found, pfdhfr, asn, table, parasites, dhfrs, avst, ile, residues, antifolate, biochemistry, inhibition, quadruple, gene, vivo, kinetic, synthetic, ala, triple, point, sites, binding, usa, university, article, falciparum, malaria, highly, purified,
Topics {✒️}
mtx-sepharose cl-6b column social media facebook n51i+c59r+s108n+i164l quadruple mutant isopropyl β-d-thiogalactoside mtx-sepharose cl-6b plasmodium falciparum dihydrofolate reductase triple mutant c59r+s108n+i164l jan-willem van ittersum triple mutant c59r+s108n+n51i triple mutants n51i+c59r+s108n dideoxy chain-termination method expression vector pet-17b pet-17b expression vector references 1 steady-state kinetic parameters pyrimethamine-resistant mutants suggested 550-bp xbai–hindiii fragment naturally occurring a16v+s108t 440-bp ndei–bglii fragment sophisticated auditory–motor learning 4 abstract materials cyc-resistant a16v+s108t mutant current emotional state kcal/mol free energy n51i+c59r+s108n+i164l librarian recommend pnas triple mutant puc-pfdhfr mtx-sepharose affinity chromatography drug-resistant parasite isolates technology development agency antifolate-resistant malaria included double mutants n51i+s108n download pdf pnas highlights newsletter national academy cyc-resistant field isolates share citation information quadruple mutant puc-pfdhfr naturally occurring mutants notes abbreviations plasmodium falciparum dhfr synthetic pfdhfr gene pfdhfr synthetic gene synthetic pfdhfr domain evolutionary tree describing discussion cyc-resistant double mutant a16v+s108t double mutant c59r mutant showed
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