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Keywords {🔍}

ire, cld, protein, unfolded, interface, residues, google, scholar, crossref, pubmed, activation, proteins, groove, fig, sequence, upr, structure, cell, crystal, data, interfaces, amino, side, binding, cells, map, chains, bip, analysis, mrna, crystals, acid, density, peptide, important, view, domain, shown, surface, activity, walter, biol, conserved, wildtype, monomer, buffer, national, control, monomers, hac,

Topics {✒️}

5 mm isopropyl-β-d-thiogalactopyranoside authors affiliations joel expressing wild-type ire1 hanging-drop vapor diffusion 32p-labeled 5′exon probe jan-willem van ittersum supporting information wild-type ire1 template notes author contributions β-galactosidase reporter assay n-terminal fusion occurred low-molecular weight species prominent antiparallel β-sheet c-terminally truncated version cld-encoding pcr fragment social media facebook x-ray diffraction data information content higher-order linear oligomers form higher-order oligomers n-terminal gst tag anisotropic b-factor correction solvent-accessible surface area sophisticated auditory–motor learning selenomethionine-substituted ire1 ld protein solvent-accessible surface current emotional state encoding β-galactosidase tables 2 figures quasi-twofold symmetric dimer pnas highlights newsletter detect wild-type higher-order quaternary structure c-terminal disordered regions disordered c-terminal region protein folding capacity ire1-cld space group acknowledgments author notes wild-type levels noncrystallographic symmetry-related density share librarian recommend pnas ire1-mediated transcriptional program pnas web site ha-tagged ire1 wild-type control wild-type cld shuttle vectors

Questions {❓}

• On tracing the unfolded protein signal back to its source in the ER, an outstanding mystery remained: How does Ire1's most N-terminal domain, which resides in the ER lumen, sense unfolded proteins?

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