
DOI . ORG {
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Title[redir]:
Identification of a nitroimidazo-oxazine-specific protein involved in PA-824 resistance in Mycobacterium tuberculosis | PNAS
Description:
PA-824 is a promising new compound for the treatment of tuberculosis that is currently undergoing human trials. Like its progenitors metronidazole and CGI-17341, PA-824 is a prodrug of the nitroimi...
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Keywords {π}
mutants, fgd, fig, google, scholar, crossref, pubmed, sciences, institute, diseases, iowa, research, national, road, resistance, sensitivity, mtb, mutant, tuberculosis, protein, dna, systems, activation, genomic, articles, information, article, analysis, university, science, view, table, microbiology, pnas, cgi, hrv, cgs, nimblegen, sequencing, novartis, tropical, singapore, allergy, infectious, helical, authors, site, genome, nitroimidazoles, paresistant,
Topics {βοΈ}
predicted flavin-nucleotide-binding site total colony-forming-unit burdens gc-rich gram-positive bacteria social media facebookx independent pa-824r/cgi-17341s mutants discrete f420-dependent nitro-reductases nitroimidazo-oxazine-specific protein involved tb drug development spontaneous oxazine-specific-resistant mutants high-gc-content organisms biotinylated goat anti-streptavidin genome-wide mutation-mapping results flavin mononucleotide-binding proteins 7-dihydro-5h-imidazo[2-1b][1 pa-824r/cgi-17341s mutants notes author contributions glucose-6-phosphate-dependent fashion 1-butanol/h2o/acetic acid jan-willem van ittersum f420h2-dependent nitro-reductases error-prone dna replication f420h2-dependent nitro-reductase mapping-phase hybridization-intensity ratio genome-wide mutational analysis stopped-flow kinetic analysis prevents opioid-induced tolerance nim-proteins encode reductases specific glucose-6-phosphate dehydrogenase copy copied isolating oxazine-specific mutants electron redox-carrier coenzyme methods bacteria siew siew pang polyclonal anti-fgd1 antibody nitroimidazo-oxazine-specific activator saturating-transposon-essentiality analysis mathematical physics spontaneous pa-824-resistant colonies significant structure-based specificity microarray-based sequencing arrays nonstringent wash buffer pa-824-free medium hydride-transfer coenzyme f420 f420-dependent nitro-reductase specific protein-ligand interaction quick search chemical genetics approach initial mapping-array results harvard medical school silica gel tlc
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