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Hepatology
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iron homeostasis, is present, but the involvement of iron in Hao1 regulation remains unclear. In this study, we found a reduction of Hao1 mRNA content in livers of rats with chronic dietary iron overload, which showed decreased IRP activity and higher ferritin expression as expected, but also induction of heme oxygenase (HOâ1), a marker of oxidative damage, and lipid peroxidation. Hao1 mRNA levels were not altered significantly in livers of rats administered doses of iron sufficient to induce ferritin expression and to repress IRP activity, but not to activate HOâ1 and to promote lipid peroxidation, as well as in the liver of ironâdeficient rats. These observations were not consistent with a postâtranscriptional downâregulation of Hao1 by iron through the IRE/IRP pathway and suggested an effect of reactive oxygen species (ROS). Indeed, a marked decrease of Hao1 mRNA was observed in the liver of rats subjected to oxidative stress induced by either glutathione depletion or postischemic reperfusion. Nuclear runâon analysis showed an effect of ROS at the transcriptional level. In conclusion, downâregulation of Hao1 expression during oxidative stress may provide a mechanism to prevent excessive H2O2 formation in liver peroxisomes and may represent the prototype of a poorly recognized but potentially relevant response to oxidative injury involving downâregulation of ROSâproducing enzymes. * Istituto di Patologia Generale, UniversitĂ di Milano, Via Mangiagalli 31, 20133 Milan, Italy. fax: (39) 0250315338; E-mail: [email protected]. Received: 6 June 2003; Accepted: 29 July 2003 Copyright Š 2003 American Association for the Study of Liver Diseases....
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Keywords {đ}
liver, hao, iron, aasld, oxidative, log, journal, downregulation, mrna, rats, access, hepatology, subscribe, issue, alerts, abstract, irp, study, content, expression, register, member, logo, articles, browse, submit, hydroxyacid, oxidase, liverspecific, peroxisomal, enzyme, buy, recognized, livers, showed, activity, ferritin, lipid, peroxidation, effect, ros, stress, copyright, american, association, diseases, text, subscribers, members, request,
Topics {âď¸}
liverâspecific peroxisomal enzyme aasld members log open access policy individual subscribers log repress irp activity institutional users access reactive oxygen species potentially relevant response artificial intelligence training higher ferritin expression induce ferritin expression oxidative injury involving hao1 messenger rna ironâresponsive element iron regulatory proteins oxidative stressâmediated oxidative stress induced rats administered doses full text access promote lipid peroxidation hao1 mrna content liver diseases ire/irp pathway rosâproducing enzymes hao1 mrna levels ironâdeficient rats journal tables register subscribe service request 800-638-3030 liver biology liver peroxisomes oxidative stress hao1 expression lipid peroxidation analysis showed journal website subscribe hao1 mrna oxidative damage liver iron homeostasis iron sufficient rats subjected oxidizes glycolate concomitant production sequence recognized key regulators heme oxygenase altered significantly postâtranscriptional
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