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Title[redir]:
Hepatology
Description:
mily, in human hepatocellular carcinoma (HCC). The Bcl–xL protein was expressed in HepG2, Hep3B, and Huh7 human hepatoma cell lines at high levels, but none of these cells expressed Bcl–2. Down–modulation of Bcl–xL by antisense oligonucleotide activated apoptosis in HepG2 cells in response to cellular stresses induced by staurosporine treatment or by serum starvation. Ectopic expression of transcriptionally active p53 alone was not sufficient for the activation of apoptosis in p53–null Hep3B cells, but apoptosis was induced when endogenous Bcl–xL was simultaneously inhibited by antisense oligonucleotide in these cells. Bcl–xL was expressed in all 20 surgically resected human HCC tissues when examined by Western blot analysis and immunohistochemistry, and levels of its expression were higher in a subset of HCC tissues than those of adjacent nontumor liver tissues or normal livers. We conclude that Bcl–xL expressed in human HCC cells inhibits apoptosis produced by various cellular stresses, such as staurosporine treatment, serum starvation, and p53 activation, and may play an important role in their survival. * FACG, Institute of Clinical Medicine and Research, Jikei University School of Medicine, 163–1 Kashiwa–shita, Kashiwa, Chiba 277–8565, Japan. fax: (81) 471–66–8635; E-mail: [email protected]. Received: 16 January 2001; Accepted: 13 April 2001 Copyright © 2001 American Association for the Study of Liver Diseases....
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Keywords {🔍}
bclxl, aasld, liver, human, apoptosis, cells, log, journal, expression, hcc, expressed, access, hepatology, subscribe, issue, alerts, member, abstract, role, study, tissues, register, logo, browse, submit, article, hepatocellular, buy, survival, bcl, hepg, hepb, levels, antisense, oligonucleotide, cellular, stresses, induced, staurosporine, treatment, serum, starvation, activation, copyright, american, association, diseases, text, subscribers, members,
Topics {✒️}
aasld members log p53–null hep3b cells open access policy human hepatocellular carcinoma individual subscribers log liver diseases institutional users access transcriptionally active p53 western blot analysis artificial intelligence training full text access bcl–xl protein endogenous bcl–xl cells expressed bcl–2 hcc tissues cellular stresses induced bcl–xl expressed journal tables register subscribe service request 800-638-3030 antisense oligonucleotide antiapoptotic member cellular stresses bcl–xl inhibit apoptosis journal hepg2 cells website subscribe p53 activation bcl–2 family apoptotic insults molecular mechanisms staurosporine treatment serum starvation simultaneously inhibited normal livers email inbox rights reserved data mining issue hcc high levels content important role ectopic expression cells apoptosis hep3b expressed induced
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