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Title[redir]:
Hepatology
Description:
ivated cell types are more sensitive than their quiescent phenotypes to apoptosis by tumor necrosis factor-related apoptosis-inducing ligand (TRAIL), we examined the expression of TRAIL death receptors (DRs) and susceptibility to TRAIL cytotoxicity in stellate cells undergoing progressive activation. A spontaneously immortalized human stellate cell line, LX-2, was analyzed during 14 days of progressive activation following plating, during which time Ξ±-smooth muscle actin (Ξ±-SMA) and a Ξ²-crystallin (markers of stellate cell activation) messenger RNA (mRNA) increased 7-fold and 5-fold, respectively. During this same interval, TRAIL-R1/DR4 and TRAIL-R2/DR5 mRNA expression increased 18-fold and 17.6-fold, although TRAIL-R2/DR5 expression was quantitatively 103-fold greater than TRAIL-R1/DR4; parallel changes occurred in TRAIL/DR5 protein expression and cellular susceptibility to TRAIL-mediated apoptosis. Similar findings were observed in primary murine stellate cells undergoing activation on a plastic surface. In conclusion, stellate cells show activation-dependent TRAIL-R2/DR5 expression and TRAIL-mediated apoptosis. Because TRAIL-R2/DR5 is not expressed by hepatocytes, TRAIL/DR5 agonists may be useful in reducing fibrosis by inducing stellate cell apoptosis. E-mail:[email protected] *Address reprint requests to: Mayo Medical School, Clinic, and Foundation, 200 First St. SW, Rochester, MN 55905. Fax: 507-284-0762. Received August 19, 2002; Accepted September 30, 2002; previously published online December 30, 2003 Copyright Β© 2003 American Association for the Study of Liver Diseases....
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Keywords {π}
stellate, cells, apoptosis, trailrdr, aasld, expression, fold, liver, log, journal, activated, trail, cell, activation, access, hepatology, subscribe, issue, alerts, abstract, trailmediated, register, member, logo, articles, browse, submit, buy, fibrosis, quiescent, susceptibility, undergoing, progressive, mrna, increased, traildr, copyright, american, association, study, diseases, text, subscribers, members, request, content, service, usa, privacy, policy,
Topics {βοΈ}
trail-r2/dr5 expression trail/dr5 protein expression trail receptor-2/death receptor-5 trail-r2/dr5 aasld members log stellate cell activation activated stellate cells trail/dr5 agonists open access policy trail-mediated apoptosis liver diseases trail death receptors activated cell types individual subscribers log trail-r1/dr4 institutional users access artificial intelligence training quantitatively 103-fold greater progressive activation full text access increased 7-fold journal tables register subscribe service request 800-638-3030 trail cytotoxicity Ξ±-sma expression journal website subscribe important mechanism reduce numbers resolution phase hepatic fibrosis observations suggest apoptotic stimuli quiescent counterparts quiescent phenotypes Ξ²-crystallin messenger rna mrna similar findings plastic surface reducing fibrosis email inbox rights reserved data mining trail apoptosis issue cellular susceptibility
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