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  6. Keywords
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We began analyzing https://www.nature.com/articles/ncomms2822, but it redirected us to https://www.nature.com/articles/ncomms2822. The analysis below is for the second page.

Title[redir]:
FTO-mediated formation of N6-hydroxymethyladenosine and N6-formyladenosine in mammalian RNA | Nature Communications
Description:
N6-methyladenosine is a prevalent internal modification in messenger RNA and non-coding RNA affecting various cellular pathways. Here we report the discovery of two additional modifications, N6-hydroxymethyladenosine (hm6A) and N6-formyladenosine (f6A), in mammalian messenger RNA. We show that FeII- and α-ketoglutarate-dependent fat mass and obesity-associated (FTO) protein oxidize N6-methyladenosine to generate N6-hydroxymethyladenosine as an intermediate modification, and N6-formyladenosine as a further oxidized product. N6-hydroxymethyladenosine and N6-formyladenosine have half-life times of ~3 h in aqueous solution under physiological relevant conditions, and are present in isolated messenger RNA from human cells as well as mouse tissues. These previously unknown modifications derived from the prevalent N6-methyladenosine in messenger RNA, formed through oxidative RNA demethylation, may dynamically modulate RNA–protein interactions to affect gene expression regulation. Internal modifications in mRNA and non-coding RNA are necessary for modulating various intracellular signalling pathways. In this study, the authors report novel modifications resulting from oxidative RNA demethylation, which regulate RNA–protein interactions affecting gene expression.

Matching Content Categories {📚}

  • Science
  • Education
  • Social Networks

Content Management System {📝}

What CMS is doi.org built with?

Custom-built

No common CMS systems were detected on Doi.org, and no known web development framework was identified.

Traffic Estimate {📈}

What is the average monthly size of doi.org audience?

🏙️ Massive Traffic: 50M - 100M visitors per month


Based on our best estimate, this website will receive around 80,479,999 visitors per month in the current month.

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How Does Doi.org Make Money? {💸}

We see no obvious way the site makes money.

The purpose of some websites isn't monetary gain; they're meant to inform, educate, or foster collaboration. Everyone has unique reasons for building websites. This could be an example. Doi.org might be plotting its profit, but the way they're doing it isn't detectable yet.

Keywords {🔍}

hma, rna, fto, article, fig, supplementary, google, scholar, cas, oxidation, nature, modifications, mrna, reaction, protein, dna, demethylation, analysis, binding, mammalian, product, min, ads, human, base, mass, conditions, structure, cells, mouse, similar, coordinates, nmethyladenosine, cell, hmc, process, nuclease, methods, model, crystal, ftomediated, modification, alkb, observed, activity, figs, simulations, content, formation, methylation,

Topics {✒️}

nature portfolio permissions reprints haeme feii/α-kg-dependent dioxygenases privacy policy body mass index china scholarship council editing open biosystems advertising electron-rich nature glen research α-ketoglutarate-dependent fat mass pollution research full access high-resolution maldi-tof-ms 2′-o-t-butyldimethylsilyl adenosine 3′-o social media n-dimethylformamidine-protected tbdms-adenosine genome-wide-association studies development rnase-free solutions nature 485 nature 419 nature 187 nature 464 nature 468 nature quantitative lc-ms/ms haem feii/α-ketoglutarate alchemical free-energy simulations30 potential m6a-rna-binding protein4 ms/ms fragmentation pattern ke-li han modulate rna–protein interactions high-resolution mass spectra buffer solutions mimicking long-term exercise training weak acid n-hydroxybenzotriazole lc-qqq-ms/ms amidine-type protecting groups alchemical free-energy simulations dna epigenetic marker22 maldi-tof-ms analysis author correspondence active cofactor α-kg 9mer single-stranded rna ms/ms detection channel gst-tagged ythdf2 protein lc-ms/ms analysis maldi-tof ionization

Schema {🗺️}

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      name:Department of Chemistry and Institute for Biophysical Dynamics, The University of Chicago, Chicago, USA
      name:Department of Chemistry and Institute for Biophysical Dynamics, The University of Chicago, Chicago, USA
      name:Department of Chemistry and Institute for Biophysical Dynamics, The University of Chicago, Chicago, USA
      name:Department of Human Genetics, The University of Chicago, Chicago, USA
      name:Department of Chemistry and Institute for Biophysical Dynamics, The University of Chicago, Chicago, USA
      name:Department of Human Genetics, The University of Chicago, Chicago, USA
      name:Department of Human Genetics, The University of Chicago, Chicago, USA
      name:State Key Laboratory of Molecular Reaction Dynamics and Center for Theoretical and Computational Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian
      name:Department of Chemistry and Theoretical Chemistry Institute, University of Wisconsin, Madison, USA
      name:Department of Chemistry and Institute for Biophysical Dynamics, The University of Chicago, Chicago, USA
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