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Keywords {🔍}

cancer, arv, androgen, prostate, pubmed, receptor, breast, cell, arvs, article, human, google, scholar, tissues, cells, exon, cas, splice, lines, transcripts, expression, splicing, variant, mdamb, shown, variants, gene, cryptic, central, copy, target, expressed, rna, fig, exons, transcript, substitution, pcr, arfl, site, cterminal, numbers, research, vcap, single, including, liver, line, products, study,

Topics {✒️}

ar-v7-specific c-terminal peptide c-terminal variant-specific peptides arv-specific c-terminal peptides c-terminal ligand-binding domain concomitant androgen-independent ar-signalling c-terminal unique peptide ar-v16-unique splicing junction ar-v15-specific splicing junction ar-v13-specific splicing junction c-terminal short peptide c-terminally adjacent residue n-terminal truncated variant n-terminal transactivation domain castration-resistant prostate cancer cell type-specific profiles arv/ar-fl expression ratios quantitative real-time pcr c-terminal peptides androgen deprivation therapy short-tandem repeat profiling ntd/dbd component attached full size image ntd/dbd domains encoded therapy-driven adaptive mechanisms x-linked reifenstein syndrome phenol red-free rpmi-1640 phenol red-free rpmi mda-mb-231 cell line mda-mb-453 cell line androgen-sensitive malignancies including conventional anti-androgenic drugs udp-glucuronosyltransferase 2b7 inhibit ar-fl activity including wild-type ar independently activate transcription arv/ar-fl ratio tissue-specific ar45 variant ligand binding domain androgen receptor variants dong gui hu ar-v7 variant protein fos-related antigen-2 alternative splice variants ar-fl copy numbers luminal androgen receptor androgen receptor splicing ar-fl transcripts compared similar ar-targeted therapies estrogen receptor alpha human androgen receptor

Questions {❓}

• Guo Z, Qiu Y (2011) A new trick of an old molecule: androgen receptor splice variants taking the stage?
• Hickey TE et al (2012) Minireview: the androgen receptor in breast tissues: growth inhibitor, tumor suppressor, oncogene?

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      description:The androgen receptor (AR) is widely expressed in human tissues and has biological function in many male and female organs. In particular, the AR plays a critical role in the biology and pathology of the prostate gland. AR activity inhibits breast growth and has pleiotropic actions in breast cancer that are subtype-dependent. Expression of AR splice variants (ARVs) and their role in prostate carcinogenesis has been elucidated in recent studies. We hypothesised that ARVs are also expressed in breast cancers and other hormone sensitive tissues. Herein, the expression of five previously identified ARV transcripts with documented transcriptional capacity (AR-V1, -V3, -V4, -V7, and -V9) was examined in 6 breast (MFM223, MDA-MB-453, MDA-MB-231, ZR75.1, MCF-7, T47D), two prostate (VCaP, LNCaP), and one liver (HepG2) cancer cell lines, a human embryonic kidney cell line (HEK293), and a panel of RNAs representing 21 different human tissues. Four ARVs (V1, V3, V7, V9) were detected to some degree in almost all cell lines and tissues. In addition, four novel ARVs containing a cryptic exon 9 (CE9) were detected in MDA-MB-453 and VCaP cells. Sequencing of ARV amplicons revealed a single nucleotide substitution within CE3 in lung and placental tissue samples that could be translated as an Ile (ATT)>Val (GTT) substitution in the AR-V7 variant protein. Collectively, these data provides insight into the potential complexity of AR transcriptional splicing events in breast cancer cell lines and diverse human tissues, thereby establishing a rationale for further exploration of ARVs in breast cancer and other human pathologies.
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