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We began analyzing https://link.springer.com/article/10.1007/s12307-010-0047-2, but it redirected us to https://link.springer.com/article/10.1007/s12307-010-0047-2. The analysis below is for the second page.

Title[redir]:
Proteomic Analysis of Differentially Expressed Proteins in Peripheral Cholangiocarcinoma | Cancer Microenvironment
Description:
Cholangiocarcinoma is an adenocarcinoma of the liver which has increased in incidence over the last thirty years to reach similar levels to other liver cancers. Diagnosis of this disease is usually late and prognosis is poor, therefore it is of great importance to identify novel candidate markers and potential early indicators of this disease as well as molecules that may be potential therapeutic targets. We have used a proteomic approach to identify differentially expressed proteins in peripheral cholangiocarcinoma cases and compared expression with paired non-tumoral liver tissue from the same patients. Two-dimensional fluorescence difference gel electrophoresis after labeling of the proteins with cyanines 3 and 5 was used to identify differentially expressed proteins. Overall, of the approximately 2,400 protein spots visualised in each gel, 172 protein spots showed significant differences in expression level between tumoral and non-tumoral tissue with p < 0.01. Of these, 100 spots corresponding to 138 different proteins were identified by mass spectrometry: 70 proteins were over-expressed whereas 68 proteins were under-expressed in tumoral samples compared to non-tumoral samples. Among the over-expressed proteins, immunohistochemistry studies confirmed an increased expression of 14-3-3 protein in tumoral cells while α-smooth muscle actin and periostin were shown to be overexpressed in the stromal myofibroblasts surrounding tumoral cells. α-Smooth muscle actin is a marker of myofibroblast differentiation and has been found to be a prognostic indicator in colon cancer while periostin may also have a role in cell adhesion, proliferation and migration and has been identified in other cancers. This underlines the role of stromal components in cancer progression and their interest for developing new diagnostic or therapeutic tools.

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Keywords {🔍}

google, scholar, pubmed, article, cas, cancer, liver, expression, proteins, cells, cell, human, hepatocellular, carcinoma, periostin, protein, res, patients, limoges, analysis, cholangiocarcinoma, tumor, electrophoresis, stromal, colorectal, gene, mol, growth, lung, darby, gel, access, proteome, oncogene, rat, france, privacy, cookies, content, proteomic, expressed, lepreux, desmoulière, early, twodimensional, muscle, proteomics, carcinomas, int, clin,

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α-smooth muscle actin alpha-smooth muscle actin pigment epithelium-derived factor month download article/chapter human tumour cells membrane-cytoskeleton organizer ezrin possesses suppressor effects hypoxia-induced cell death chronic liver injury situ cdna-mrna hybridization p53 c-terminus bound serum l-lactate dehydrogenase enzyme-altered liver cells translation factor mrnas karine vuillier-devillers human alpha-actinin full article pdf alpha-1-antitrypsin deficiency periostin promotes invasiveness s-adenosylmethionine pro-inflammatory cytokines adrenomedullin binding protein-1 differentially expressed proteins biliary malignancy serological proteome analysis tumor stroma liver-specific trait charles balabaud faculté des sciences privacy choices/manage cookies peripheral cholangiocarcinoma cases autoantibody signatures defined dimensional gel electrophoresis region frequently altered gene expression profiling positional expression profiling pancreatic cancer cells related subjects lung cancer cells université de limoges proteomic profiling potential early indicators pancreatic acinar cells human colon cancer contrat renforcé recherche metastatic liver carcinomas primary liver carcinomas human hepatocellular carcinoma tumor suppressive activity liver carcinoma cells

Questions {❓}

Bonney GK, Craven RA, Prasad R, Melcher AF, Selby PJ, Banks RE (2008) Circulating markers of biliary malignancy: opportunities in proteomics?

Schema {🗺️}

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