
DOI . ORG {
}
Title[redir]:
Tumor-infiltrating regulatory T cells, CD8/Treg ratio, and cancer stem cells are correlated with lymph node metastasis in patients with early breast cancer | Breast Cancer
Description:
Background Tumor-infiltrating lymphocytes are an important component of the tumor microenvironment (TME) in breast cancer. They have been linked with tumor pathogenesis in advanced stages. However, little is known about their contribution in early phases. In this study, we analyzed the infiltration of leukocytes and cancer stem cells (CSC) in tumors from patients with early breast cancer. Methods Samples of blood and tumor tissue from 30 patients with breast cancer were collected, and the number of dendritic cells (DC), T cells, and CSC were analyzed by flow cytometry. Results Tumor-infiltrating CD4 and CD8 T cells expressed higher levels of cytotoxic T-lymphocyte-associated antigen 4 (CTLA-4) compared with peripheral T cells. Regulatory T cells (Treg) were enriched in tumors and overexpressed glucocorticoid-induced TNFR-related protein and CTLA-4. Tumor Treg had a positive correlation with the amount of myeloid DC (mDC) and disease progression. The CD8/Treg ratio was associated with lymph node metastasis and tumor stages. The main subset of DC in early breast tumors was mDC, while plasmacytoid DC were almost absent. CSC were present in most tumors with higher frequencies in patients with lymph node metastasis. CSC were also associated with the amount of tumor-infiltrating Treg. Conclusion Early breast cancer has an inflammatory milieu characterized by mDC, Treg, and CSC infiltration. The frequencies of Treg, CSC and CD8/Treg ratio were associated with disease progression. The composition of leukocytes and the presence of CSC in early breast tumors should be considered for the development of new therapeutic approaches.
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Keywords {🔍}
cancer, article, cells, breast, google, scholar, cas, regulatory, dendritic, tumor, méxico, tumorinfiltrating, patients, pedrozagonzalez, stem, early, plasmacytoid, metastasis, cell, csc, tumors, access, human, clin, res, ratio, lymphocytes, ctla, prognostic, privacy, cookies, content, lymph, node, garciaromo, alexander, infiltration, treg, progression, oncoimmunology, med, immunol, oncol, del, nacional, analysis, information, publish, research, search,
Topics {✒️}
jose rafael jimenez-flores victor hugo rosales-garcia alexander pedroza-gonzalez centro de investigación month download article/chapter cancer stem/initiating cells article solis-castillo gina stella garcia-romo ciudad de méxico estado de méxico proyectos de investigación cells/cancer-initiating cells project numbers ia204515/ra204515 adolfo rene mendez-cruz human cancer stem project papiit ia204515/ra204515 cancer stem cells breast tumor microenvironment �programa de apoyo de los barrios plasmacytoid dendritic cells human breast cancer garcia-castillo kg tumor-infiltrating lymphocytes alejandro gallardo flores breast cancer cells early breast cancer institutional research committee invasive breast cancer pedroza-gonzalez related subjects tumor-infiltrating regulatory full article pdf metastatic breast cancer breast cancer treated fes iztacala dendritic cell infiltration cell infiltrates varies privacy choices/manage cookies metastatic liver cancer breast cancer res human liver tumors ifnalpha production promote tumor-infiltrating treg early breast tumors t-regulatory cells breast cancer patients nat cell biol immune responses cancer immunol immunother
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headline:Tumor-infiltrating regulatory T cells, CD8/Treg ratio, and cancer stem cells are correlated with lymph node metastasis in patients with early breast cancer
description:Tumor-infiltrating lymphocytes are an important component of the tumor microenvironment (TME) in breast cancer. They have been linked with tumor pathogenesis in advanced stages. However, little is known about their contribution in early phases. In this study, we analyzed the infiltration of leukocytes and cancer stem cells (CSC) in tumors from patients with early breast cancer. Samples of blood and tumor tissue from 30 patients with breast cancer were collected, and the number of dendritic cells (DC), T cells, and CSC were analyzed by flow cytometry. Tumor-infiltrating CD4 and CD8 T cells expressed higher levels of cytotoxic T-lymphocyte-associated antigen 4 (CTLA-4) compared with peripheral T cells. Regulatory T cells (Treg) were enriched in tumors and overexpressed glucocorticoid-induced TNFR-related protein and CTLA-4. Tumor Treg had a positive correlation with the amount of myeloid DC (mDC) and disease progression. The CD8/Treg ratio was associated with lymph node metastasis and tumor stages. The main subset of DC in early breast tumors was mDC, while plasmacytoid DC were almost absent. CSC were present in most tumors with higher frequencies in patients with lymph node metastasis. CSC were also associated with the amount of tumor-infiltrating Treg. Early breast cancer has an inflammatory milieu characterized by mDC, Treg, and CSC infiltration. The frequencies of Treg, CSC and CD8/Treg ratio were associated with disease progression. The composition of leukocytes and the presence of CSC in early breast tumors should be considered for the development of new therapeutic approaches.
datePublished:2020-03-16T00:00:00Z
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Early breast cancer
Regulatory T cells
Dendritic cells
Cancer stem cells
Surgical Oncology
Oncology
Surgery
Cancer Research
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description:Tumor-infiltrating lymphocytes are an important component of the tumor microenvironment (TME) in breast cancer. They have been linked with tumor pathogenesis in advanced stages. However, little is known about their contribution in early phases. In this study, we analyzed the infiltration of leukocytes and cancer stem cells (CSC) in tumors from patients with early breast cancer. Samples of blood and tumor tissue from 30 patients with breast cancer were collected, and the number of dendritic cells (DC), T cells, and CSC were analyzed by flow cytometry. Tumor-infiltrating CD4 and CD8 T cells expressed higher levels of cytotoxic T-lymphocyte-associated antigen 4 (CTLA-4) compared with peripheral T cells. Regulatory T cells (Treg) were enriched in tumors and overexpressed glucocorticoid-induced TNFR-related protein and CTLA-4. Tumor Treg had a positive correlation with the amount of myeloid DC (mDC) and disease progression. The CD8/Treg ratio was associated with lymph node metastasis and tumor stages. The main subset of DC in early breast tumors was mDC, while plasmacytoid DC were almost absent. CSC were present in most tumors with higher frequencies in patients with lymph node metastasis. CSC were also associated with the amount of tumor-infiltrating Treg. Early breast cancer has an inflammatory milieu characterized by mDC, Treg, and CSC infiltration. The frequencies of Treg, CSC and CD8/Treg ratio were associated with disease progression. The composition of leukocytes and the presence of CSC in early breast tumors should be considered for the development of new therapeutic approaches.
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Early breast cancer
Regulatory T cells
Dendritic cells
Cancer stem cells
Surgical Oncology
Oncology
Surgery
Cancer Research
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