
DOI . ORG {
}
Title[redir]:
Expression of GPC3 protein and its significance in lung squamous cell carcinoma | Medical Oncology
Description:
The purpose of this study was to investigate GPC3 gene expression in lung squamous cell carcinoma tissue and its correlation with clinical and tumor characteristics. Using RTāPCR, the presence of GPC3 gene expression was detected in cancer tissue and adjacent normal tissue in 66 cases of lung squamous cell carcinoma and positive rates were calculated. Using Western blot, changes in GPC3 protein expression were detected in lung squamous cell carcinoma and adjacent normal tissues. The percentage of tissue samples expressing GPC3 mRNA was significantly higher in lung squamous cell carcinoma than in adjacent normal tissue (P < 0.05). This percentage was also significantly higher for cases with lymph node metastasis than for those without lymph node metastasis (P < 0.05). Further, the percentage of samples expressing GPC3 mRNA was higher with lowering degrees of tumor differentiation (P < 0.05). Rates of GPC3 expression were, however, independent of patient gender, age, and tumor size (P > 0.05). The expression of GPC3 protein in lung squamous cell carcinoma was significantly higher than that in adjacent normal tissues (P < 0.05). The expression in cases with lymph node metastasis was significantly higher than in those without lymph node metastasis (P < 0.05), and GPC3 protein expression increased with lowering degrees of tumor differentiation (P < 0.05). Further investigation is warranted for the association of initiation, development, invasion, and metastasis of disease.
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article, google, scholar, pubmed, cas, expression, glypican, lung, cell, cancer, gpc, carcinoma, squamous, shanghai, gene, tumor, filmus, protein, tissue, metastasis, lin, access, res, privacy, cookies, content, higher, adenocarcinoma, pathol, breast, information, publish, search, qiang, xiong, adjacent, normal, cases, significantly, lymph, node, biol, hepatocellular, signaling, clin, chest, analysis, data, log, journal,
Topics {āļø}
hui-fang shaĀ &Ā jiu-xian feng simpson-golabi-behmel overgrowth syndrome cell line-specific manner month download article/chapter li-wen xiong western blot clear cell adenocarcinoma hui-fang sha jiu-xian feng rtāpcr related subjects full article pdf privacy choices/manage cookies human breast cancer guo-liang bao human hepatocellular carcinoma clin cancer res lung adenocarcinoma jun-feng gen lam wl adjacent normal tissue nonsmokers identifies gpc3 gpc3 gene expression european economic area garay-malpartida hm benign hepatic disorders hughes-benzie rm xiang yy gene expression profiling tissue microarray analysis article lin check access instant access gpc3 protein expression p38mapk signaling pathways growth signaling molecules conditions privacy policy adjacent normal tissues pediatr hematol oncol oligonucleotide microarray analysis cell biol canonical wnt pathway shanghai jiaotong university lymph node metastasis accepting optional cookies xu-feng pan promising diagnostic marker glypican-3 protein expression glypican-3 induces alterations cancer tissue
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headline:Expression of GPC3 protein and its significance in lung squamous cell carcinoma
description:The purpose of this study was to investigate GPC3 gene expression in lung squamous cell carcinoma tissue and its correlation with clinical and tumor characteristics. Using RTāPCR, the presence of GPC3 gene expression was detected in cancer tissue and adjacent normal tissue in 66 cases of lung squamous cell carcinoma and positive rates were calculated. Using Western blot, changes in GPC3 protein expression were detected in lung squamous cell carcinoma and adjacent normal tissues. The percentage of tissue samples expressing GPC3 mRNA was significantly higher in lung squamous cell carcinoma than in adjacent normal tissue (PĀ <Ā 0.05). This percentage was also significantly higher for cases with lymph node metastasis than for those without lymph node metastasis (PĀ <Ā 0.05). Further, the percentage of samples expressing GPC3 mRNA was higher with lowering degrees of tumor differentiation (PĀ <Ā 0.05). Rates of GPC3 expression were, however, independent of patient gender, age, and tumor size (PĀ >Ā 0.05). The expression of GPC3 protein in lung squamous cell carcinoma was significantly higher than that in adjacent normal tissues (PĀ <Ā 0.05). The expression in cases with lymph node metastasis was significantly higher than in those without lymph node metastasis (PĀ <Ā 0.05), and GPC3 protein expression increased with lowering degrees of tumor differentiation (PĀ <Ā 0.05). Further investigation is warranted for the association of initiation, development, invasion, and metastasis of disease.
datePublished:2011-05-10T00:00:00Z
dateModified:2011-05-10T00:00:00Z
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GPC3
Lung squamous cell carcinoma
RTāPCR
Western blot
Oncology
Hematology
Pathology
Internal Medicine
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headline:Expression of GPC3 protein and its significance in lung squamous cell carcinoma
description:The purpose of this study was to investigate GPC3 gene expression in lung squamous cell carcinoma tissue and its correlation with clinical and tumor characteristics. Using RTāPCR, the presence of GPC3 gene expression was detected in cancer tissue and adjacent normal tissue in 66 cases of lung squamous cell carcinoma and positive rates were calculated. Using Western blot, changes in GPC3 protein expression were detected in lung squamous cell carcinoma and adjacent normal tissues. The percentage of tissue samples expressing GPC3 mRNA was significantly higher in lung squamous cell carcinoma than in adjacent normal tissue (PĀ <Ā 0.05). This percentage was also significantly higher for cases with lymph node metastasis than for those without lymph node metastasis (PĀ <Ā 0.05). Further, the percentage of samples expressing GPC3 mRNA was higher with lowering degrees of tumor differentiation (PĀ <Ā 0.05). Rates of GPC3 expression were, however, independent of patient gender, age, and tumor size (PĀ >Ā 0.05). The expression of GPC3 protein in lung squamous cell carcinoma was significantly higher than that in adjacent normal tissues (PĀ <Ā 0.05). The expression in cases with lymph node metastasis was significantly higher than in those without lymph node metastasis (PĀ <Ā 0.05), and GPC3 protein expression increased with lowering degrees of tumor differentiation (PĀ <Ā 0.05). Further investigation is warranted for the association of initiation, development, invasion, and metastasis of disease.
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