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We began analyzing https://link.springer.com/article/10.1007/s12031-017-0885-1, but it redirected us to https://link.springer.com/article/10.1007/s12031-017-0885-1. The analysis below is for the second page.

Title[redir]:
The Anti-Aging Effect of Erythropoietin via the ERK/Nrf2-ARE Pathway in Aging Rats | Journal of Molecular Neuroscience
Description:
Erythropoietin (EPO) has a neuroprotective effect and can resist aging, which most likely occur through EPO increasing the activity of antioxidant enzymes and scavenging free radicals. In this study, we verified the anti-aging function of EPO and discussed the mechanism occurring through the extracellular signal-regulated kinase (ERK)/NF-E2-related factor 2 (Nrf2)-ARE pathway. A rat model of aging was induced by the continuous subcutaneous injection of 5 % d-galactose for 6 weeks. At the beginning of the sixth week, physiological saline or EPO was administered twice per day through a lateral ventricle system for a total of 7 days. In one group, 2 μl PD98059 was administered 30 min before EPO. Learning and memory ability were analyzed with the Morris water maze system. HE staining was used to observe the morphological changes in the neurons in the hippocampus, and immunohistochemical staining as well as Western blots were carried out to detect the expression of ERK for each group of rats and the expression of phosphorylated-ERK (P-ERK), Nrf2, and superoxide dismutase (SOD). Real-Time PCR was carried out to detect the amount of Nrf2 mRNA and the KEAP1 mRNA expression. EPO can significantly improve learning and memory ability in aging rats and can provide protection against aging by improving the hippocampus morphology. Immunohistochemical staining and Western blots showed P-ERK, Nrf2, and Cu-Zn SOD decreases in aging rats compared to the normal group, while the expression for those proteins increased after EPO intervention. PD98059 inhibited the enhanced expression of P-ERK, Nrf2, and Cu-Zn SOD induced by EPO. Real-Time PCR results suggested that the trend of Nrf2mRNA expression was the same as that for the proteins, which confirmed that the enhancement occurred at the gene level. As such, EPO can significantly resist or delay aging and protect the brain by reducing oxidative stress. The most likely mechanism is that EPO can promote the ERK/Nrf2-ARE pathway in aging rats and that PD98059 can inhibit that process. These findings may facilitate further studies on the mechanism of aging and applications for the neuroprotective properties of EPO for clinical treatments.

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article, pubmed, google, scholar, cas, aging, erythropoietin, epo, pathway, rats, nrf, oxidative, stress, expression, privacy, cookies, content, journal, wang, neuroprotective, induced, memory, brain, disease, med, publish, research, search, antiaging, effect, jiaxin, zhao, chen, zhang, free, perk, sod, access, cell, diseases, china, clin, res, function, data, information, log, molecular, neuroscience, erknrfare,

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cu-zn sod decreases cu-zn sod induced month download article/chapter /nf-e2-related factor 2 extracellular signal-regulated kinase nf-kappab signalling cascades age-related failure brain-derived erythropoietin protects sh-sy5y cells rhepo affects apoptosis anti-aging effect full article pdf real-time pcr anti-aging function privacy choices/manage cookies related subjects nrf2-keap1 signaling pathway aging-related diseases reactive oxygen species p-erk reactive nitrogen species van berckel bn de la sayette lateral ventricle system cell defense system article wu european economic area continuous subcutaneous injection inverse cancer risk rodriguez-franco mi engel jd free radical timer shorten animal lifespan conditions privacy policy article journal focal cerebral ischemia nrf2 transcriptionally activates scavenging free radicals amyloid-negative alzheimer neurogenic small molecules antioxidant response element erythropoietin hyporesponsiveness anemia aging rats published aging rats compared reducing oxidative stress check access counteracting oxidative stress instant access article log accepting optional cookies

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Engel PA (2016) Is age-related failure of metabolic reprogramming a principal mediator in idiopathic Parkinson’s disease?
Liochev SI (2015) Which is the most significant cause of aging?
Sanz A (2016) Mitochondrial reactive oxygen species: do they extend or shorten animal lifespan?

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