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We began analyzing https://link.springer.com/article/10.1007/s11010-010-0473-y, but it redirected us to https://link.springer.com/article/10.1007/s11010-010-0473-y. The analysis below is for the second page.

Title[redir]:
Demethylating agent 5-aza-2-deoxycytidine enhances susceptibility of breast cancer cells to anticancer agents | Molecular and Cellular Biochemistry
Description:
DNA methylation plays an important role in regulation of gene expression and is increasingly being recognized as a determinant of chemosensitivity of human cancers. With the aim of improving the chemotherapeutic efficacy of breast carcinoma, the effect of DNA methyltransferase inhibitor, 5-Aza-2′-deoxycytidine (5-aza-CdR), on the chemosensitivity of anticancer drugs was investigated. The cytotoxicity of paclitaxel (PTX), adriamycin (ADR), and 5-fluorouracil (5-FU) was analyzed against human breast cancer cell lines, MDA MB 231 and MCF 7 cell lines using the MTT assay, and the synergy of 5-aza-CdR and these agents was determined by Drewinko’s fraction method. The effects of each single agent or the combined treatment on cell cycle arrest were analyzed by flow cytometric analysis. We also investigated the effect of each single agent or the combined treatment of anticancer drugs with 5-aza-CdR on the methylation status of the selected genes by methylation specific PCR. In MDA MB 231 cells, a synergistic antiproliferative effect was observed with a combination of 10 μM 5-aza-CdR and these three anticancer drugs, while in MCF 7 cells, a semiadditive effect was observed. Treatment with 5-aza-CdR and anticancer drug resulted in partial demethylation of a panel of genes including RARβ2, Slit2, GSTP1, and MGMT. Based on these findings, we propose that 5-aza-CdR enhances the chemosensitivity of anticancer drugs in breast cancer cells and may be a promising approach for increasing the chemotherapeutic potential of these anticancer agents for more effective management of breast carcinomas.

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Keywords {🔍}

article, cancer, google, scholar, pubmed, cas, breast, anticancer, azadeoxycytidine, cell, methylation, human, agent, cells, drugs, treatment, dna, expression, azacdr, lines, privacy, cookies, content, agents, sharma, ranju, ralhan, gene, carcinoma, effect, access, res, lung, information, publish, search, demethylating, enhances, mirza, gayatri, chemosensitivity, drug, lee, analysis, data, log, journal, research, biochemistry, sameer,

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o6-methylguanine-dna methyltransferase gene month download article/chapter low-dose 5-aza-2′-deoxycytidine 5-aza-2′-deoxycytidine sensitizes hepatoma depressing p-glycoprotein activity promotes tnf-mediated apoptosis cellular biochemistry aims anticancer drug resulted 5-aza-cdr enhances full article pdf privacy choices/manage cookies dna methyltransferase inhibitor cell cycle arrest methylation specific pcr breast cancer cells prostate cancer detection ranju ralhan anti-cancer strategies anticancer agents published 10 μm 5-aza-cdr cyclin d2 expression gstp1 methylation analysis european economic area carbon metabolism pathways nonclonal neutrophil responses da silva nf tumor suppressor activity p53-induced apoptosis histone deacetylase inhibitor mount sinai hospital dna methylation article molecular article mirza 5-aza-2′-deoxycytidine genes including rarβ2 conditions privacy policy flow cytometric analysis check access drug combinations instant access gayatri sharma author information authors breast carcinoma accepting optional cookies drosophila slit2 gene single agent synergic antiproliferative effect combination chemotherapy article log synergistic antiproliferative effect

Schema {🗺️}

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