Here's how DOI.ORG makes money* and how much!

*Please read our disclaimer before using our estimates.
Loading...

DOI . ORG {}

  1. Analyzed Page
  2. Matching Content Categories
  3. CMS
  4. Monthly Traffic Estimate
  5. How Does Doi.org Make Money
  6. Keywords
  7. Topics
  8. Questions
  9. Schema
  10. External Links
  11. Analytics And Tracking
  12. Libraries
  13. Hosting Providers
  14. CDN Services

We began analyzing https://link.springer.com/article/10.1007/s10495-008-0200-2, but it redirected us to https://link.springer.com/article/10.1007/s10495-008-0200-2. The analysis below is for the second page.

Title[redir]:
The tubulin-depolymerising agent combretastatin-4 induces ectopic aster assembly and mitotic catastrophe in lung cancer cells H460 | Apoptosis
Description:
The relationship between microtubular dynamics, dismantling of pericentriolar components and induction of apoptosis was analysed after exposure of H460 non-small lung cancer cells to anti-mitotic drugs. The microtubule destabilising agent, combretastatin-A4 (CA-4) led to microtubular array disorganization, arrest in mitosis and abnormal metaphases, accompanied by the presence of numerous centrosome-independent “star-like” structures containing tubulin and aggregates of pericentrosomal matrix components like γ-tubulin, pericentrin and ninein, whereas the structural integrity of centrioles was not affected by treatment. On the contrary, in condition of prolonged exposure or high concentrations of CA-4 such aggregates never formed. Treatment with 7.5 nM CA-4, which produced a high frequency “star-like” aggregates, was accompanied by mitotic catastrophe commitment characterized by translocation of the proapoptotic Bim protein to mitochondria activation of caspases-3/9 and DNA fragmentation as a result of either prolonged metaphase arrest or attempt of cells to divide. Drug concentrations which fail to block cells at mitosis were also unable to activate apotosis. A detailed time-course analysis of cell cycle arrest and apoptosis indicated that after CA-4 washout the number of metaphases with “star-like” structures decreased as a function of time and arrested cells proceeded in anaphase. After 4 h, the multiple α- and γ-tubulin aggregates coalesced into two well-defined spindles in a bipolar mitotic spindle organization. Overall, our findings suggest that the maintenance of microtubular integrity plays a relevant role in stabilising the pericentriolar matrix, whose dismantling can be associated with apoptosis after exposure to microtubule depolymerising agents.

Matching Content Categories {📚}

  • Education
  • Telecommunications
  • Science

Content Management System {📝}

What CMS is doi.org built with?

Custom-built

No common CMS systems were detected on Doi.org, and no known web development framework was identified.

Traffic Estimate {📈}

What is the average monthly size of doi.org audience?

🌠 Phenomenal Traffic: 5M - 10M visitors per month


Based on our best estimate, this website will receive around 8,426,036 visitors per month in the current month.

check SE Ranking
check Ahrefs
check Similarweb
check Ubersuggest
check Semrush

How Does Doi.org Make Money? {💸}

We're unsure how the site profits.

Earning money isn't the goal of every website; some are designed to offer support or promote social causes. People have different reasons for creating websites. This might be one such reason. Doi.org might be earning cash quietly, but we haven't detected the monetization method.

Keywords {🔍}

google, scholar, cas, article, pubmed, cell, cells, mitotic, biol, microtubule, centrosome, apoptosis, catastrophe, sci, gammatubulin, cancer, spindle, role, agents, microtubules, combretastatin, access, centrosomal, privacy, cookies, content, induces, cenciarelli, tanzarella, vitale, antoccia, arrest, mitosis, ninein, death, rev, centrosomes, bornens, function, publish, search, agent, lung, crateri, arancia, dynamics, tubulin, aggregates, γtubulin, pericentrin,

Topics {✒️}

month download article/chapter multidrug-resistant cancer therapy radiation-induced cell death radiation-induced mitotic catastrophe diazepam-induced mitotic failure microtubule-organizing centers abnormal microtubule minus-end anchorage gamma-tubulin complexes x-irradiated mammalian cells stefania meschini & giuseppe arancia microtubule depolymerising agents arsenite-induced mitotic arrest microtubule destabilising agent numerous centrosome-independent “star proapoptotic bim protein γ-tubulin aggregates coalesced p53 transcription-dependent full article pdf gamma-tubulin form microtubulin binding sites article apoptosis aims privacy choices/manage cookies microtubule-interfering agents microtubule-damaging agents gamma-tubulin distribution cell cycle arrest kinetochore-microtubule attachment tubulin superfamily encoded alpha tubulin acetylation ilio vitale article cenciarelli minus-end capture antonio antoccia multinucleated cells induced combretastatin-a4 incresased microtubule stability independent processes linked sigma-tau anti-mitotic drugs developing anticancer agents microtubular array disorganization arrested cells proceeded european economic area microtubular integrity plays n-substituted isatins vande woude gf adenomatous polyposis coli crest patient sera mitochondrial membrane permeabilization università roma tre

Questions {❓}

  • Roninson IB, Broude EV, Chang BD (2007) If not apoptosis then what?

Schema {🗺️}

WebPage:
      mainEntity:
         headline:The tubulin-depolymerising agent combretastatin-4 induces ectopic aster assembly and mitotic catastrophe in lung cancer cells H460
         description:The relationship between microtubular dynamics, dismantling of pericentriolar components and induction of apoptosis was analysed after exposure of H460 non-small lung cancer cells to anti-mitotic drugs. The microtubule destabilising agent, combretastatin-A4 (CA-4) led to microtubular array disorganization, arrest in mitosis and abnormal metaphases, accompanied by the presence of numerous centrosome-independent “star-like” structures containing tubulin and aggregates of pericentrosomal matrix components like γ-tubulin, pericentrin and ninein, whereas the structural integrity of centrioles was not affected by treatment. On the contrary, in condition of prolonged exposure or high concentrations of CA-4 such aggregates never formed. Treatment with 7.5 nM CA-4, which produced a high frequency “star-like” aggregates, was accompanied by mitotic catastrophe commitment characterized by translocation of the proapoptotic Bim protein to mitochondria activation of caspases-3/9 and DNA fragmentation as a result of either prolonged metaphase arrest or attempt of cells to divide. Drug concentrations which fail to block cells at mitosis were also unable to activate apotosis. A detailed time-course analysis of cell cycle arrest and apoptosis indicated that after CA-4 washout the number of metaphases with “star-like” structures decreased as a function of time and arrested cells proceeded in anaphase. After 4 h, the multiple α- and γ-tubulin aggregates coalesced into two well-defined spindles in a bipolar mitotic spindle organization. Overall, our findings suggest that the maintenance of microtubular integrity plays a relevant role in stabilising the pericentriolar matrix, whose dismantling can be associated with apoptosis after exposure to microtubule depolymerising agents.
         datePublished:2008-04-02T00:00:00Z
         dateModified:2008-04-02T00:00:00Z
         pageStart:659
         pageEnd:669
         sameAs:https://doi.org/10.1007/s10495-008-0200-2
         keywords:
            CA-4
            Centrosome
            Ninein
            Gamma-tubulin
            Pericentrin
            Mitotic catastrophe
            Bim
            Cancer Research
            Cell Biology
            Oncology
            Biochemistry
            general
            Virology
         image:
            https://media.springernature.com/lw1200/springer-static/image/art%3A10.1007%2Fs10495-008-0200-2/MediaObjects/10495_2008_200_Fig1_HTML.gif
            https://media.springernature.com/lw1200/springer-static/image/art%3A10.1007%2Fs10495-008-0200-2/MediaObjects/10495_2008_200_Fig2_HTML.gif
            https://media.springernature.com/lw1200/springer-static/image/art%3A10.1007%2Fs10495-008-0200-2/MediaObjects/10495_2008_200_Fig3_HTML.gif
            https://media.springernature.com/lw1200/springer-static/image/art%3A10.1007%2Fs10495-008-0200-2/MediaObjects/10495_2008_200_Fig4_HTML.gif
            https://media.springernature.com/lw1200/springer-static/image/art%3A10.1007%2Fs10495-008-0200-2/MediaObjects/10495_2008_200_Fig5_HTML.gif
         isPartOf:
            name:Apoptosis
            issn:
               1573-675X
               1360-8185
            volumeNumber:13
            type:
               Periodical
               PublicationVolume
         publisher:
            name:Springer US
            logo:
               url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
               type:ImageObject
            type:Organization
         author:
               name:Chiara Cenciarelli
               affiliation:
                     name:University “Roma Tre”
                     address:
                        name:Department of Biology, University “Roma Tre”, Rome, Italy
                        type:PostalAddress
                     type:Organization
               type:Person
               name:Caterina Tanzarella
               affiliation:
                     name:University “Roma Tre”
                     address:
                        name:Department of Biology, University “Roma Tre”, Rome, Italy
                        type:PostalAddress
                     type:Organization
               type:Person
               name:Ilio Vitale
               affiliation:
                     name:University “Roma Tre”
                     address:
                        name:Department of Biology, University “Roma Tre”, Rome, Italy
                        type:PostalAddress
                     type:Organization
               type:Person
               name:Claudio Pisano
               affiliation:
                     name:SIGMA-TAU Industrie Farmaceutiche Riunite S.p.A
                     address:
                        name:SIGMA-TAU Industrie Farmaceutiche Riunite S.p.A, Pomezia, Italy
                        type:PostalAddress
                     type:Organization
               type:Person
               name:Pasqualina Crateri
               affiliation:
                     name:Istituto Superiore di Sanità
                     address:
                        name:Department of Technology and Health, Istituto Superiore di Sanità, Rome, Italy
                        type:PostalAddress
                     type:Organization
               type:Person
               name:Stefania Meschini
               affiliation:
                     name:Istituto Superiore di Sanità
                     address:
                        name:Department of Technology and Health, Istituto Superiore di Sanità, Rome, Italy
                        type:PostalAddress
                     type:Organization
               type:Person
               name:Giuseppe Arancia
               affiliation:
                     name:Istituto Superiore di Sanità
                     address:
                        name:Department of Technology and Health, Istituto Superiore di Sanità, Rome, Italy
                        type:PostalAddress
                     type:Organization
               type:Person
               name:Antonio Antoccia
               affiliation:
                     name:University “Roma Tre”
                     address:
                        name:Department of Biology, University “Roma Tre”, Rome, Italy
                        type:PostalAddress
                     type:Organization
               email:[email protected]
               type:Person
         isAccessibleForFree:
         hasPart:
            isAccessibleForFree:
            cssSelector:.main-content
            type:WebPageElement
         type:ScholarlyArticle
      context:https://schema.org
ScholarlyArticle:
      headline:The tubulin-depolymerising agent combretastatin-4 induces ectopic aster assembly and mitotic catastrophe in lung cancer cells H460
      description:The relationship between microtubular dynamics, dismantling of pericentriolar components and induction of apoptosis was analysed after exposure of H460 non-small lung cancer cells to anti-mitotic drugs. The microtubule destabilising agent, combretastatin-A4 (CA-4) led to microtubular array disorganization, arrest in mitosis and abnormal metaphases, accompanied by the presence of numerous centrosome-independent “star-like” structures containing tubulin and aggregates of pericentrosomal matrix components like γ-tubulin, pericentrin and ninein, whereas the structural integrity of centrioles was not affected by treatment. On the contrary, in condition of prolonged exposure or high concentrations of CA-4 such aggregates never formed. Treatment with 7.5 nM CA-4, which produced a high frequency “star-like” aggregates, was accompanied by mitotic catastrophe commitment characterized by translocation of the proapoptotic Bim protein to mitochondria activation of caspases-3/9 and DNA fragmentation as a result of either prolonged metaphase arrest or attempt of cells to divide. Drug concentrations which fail to block cells at mitosis were also unable to activate apotosis. A detailed time-course analysis of cell cycle arrest and apoptosis indicated that after CA-4 washout the number of metaphases with “star-like” structures decreased as a function of time and arrested cells proceeded in anaphase. After 4 h, the multiple α- and γ-tubulin aggregates coalesced into two well-defined spindles in a bipolar mitotic spindle organization. Overall, our findings suggest that the maintenance of microtubular integrity plays a relevant role in stabilising the pericentriolar matrix, whose dismantling can be associated with apoptosis after exposure to microtubule depolymerising agents.
      datePublished:2008-04-02T00:00:00Z
      dateModified:2008-04-02T00:00:00Z
      pageStart:659
      pageEnd:669
      sameAs:https://doi.org/10.1007/s10495-008-0200-2
      keywords:
         CA-4
         Centrosome
         Ninein
         Gamma-tubulin
         Pericentrin
         Mitotic catastrophe
         Bim
         Cancer Research
         Cell Biology
         Oncology
         Biochemistry
         general
         Virology
      image:
         https://media.springernature.com/lw1200/springer-static/image/art%3A10.1007%2Fs10495-008-0200-2/MediaObjects/10495_2008_200_Fig1_HTML.gif
         https://media.springernature.com/lw1200/springer-static/image/art%3A10.1007%2Fs10495-008-0200-2/MediaObjects/10495_2008_200_Fig2_HTML.gif
         https://media.springernature.com/lw1200/springer-static/image/art%3A10.1007%2Fs10495-008-0200-2/MediaObjects/10495_2008_200_Fig3_HTML.gif
         https://media.springernature.com/lw1200/springer-static/image/art%3A10.1007%2Fs10495-008-0200-2/MediaObjects/10495_2008_200_Fig4_HTML.gif
         https://media.springernature.com/lw1200/springer-static/image/art%3A10.1007%2Fs10495-008-0200-2/MediaObjects/10495_2008_200_Fig5_HTML.gif
      isPartOf:
         name:Apoptosis
         issn:
            1573-675X
            1360-8185
         volumeNumber:13
         type:
            Periodical
            PublicationVolume
      publisher:
         name:Springer US
         logo:
            url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
            type:ImageObject
         type:Organization
      author:
            name:Chiara Cenciarelli
            affiliation:
                  name:University “Roma Tre”
                  address:
                     name:Department of Biology, University “Roma Tre”, Rome, Italy
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Caterina Tanzarella
            affiliation:
                  name:University “Roma Tre”
                  address:
                     name:Department of Biology, University “Roma Tre”, Rome, Italy
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Ilio Vitale
            affiliation:
                  name:University “Roma Tre”
                  address:
                     name:Department of Biology, University “Roma Tre”, Rome, Italy
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Claudio Pisano
            affiliation:
                  name:SIGMA-TAU Industrie Farmaceutiche Riunite S.p.A
                  address:
                     name:SIGMA-TAU Industrie Farmaceutiche Riunite S.p.A, Pomezia, Italy
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Pasqualina Crateri
            affiliation:
                  name:Istituto Superiore di Sanità
                  address:
                     name:Department of Technology and Health, Istituto Superiore di Sanità, Rome, Italy
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Stefania Meschini
            affiliation:
                  name:Istituto Superiore di Sanità
                  address:
                     name:Department of Technology and Health, Istituto Superiore di Sanità, Rome, Italy
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Giuseppe Arancia
            affiliation:
                  name:Istituto Superiore di Sanità
                  address:
                     name:Department of Technology and Health, Istituto Superiore di Sanità, Rome, Italy
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Antonio Antoccia
            affiliation:
                  name:University “Roma Tre”
                  address:
                     name:Department of Biology, University “Roma Tre”, Rome, Italy
                     type:PostalAddress
                  type:Organization
            email:[email protected]
            type:Person
      isAccessibleForFree:
      hasPart:
         isAccessibleForFree:
         cssSelector:.main-content
         type:WebPageElement
["Periodical","PublicationVolume"]:
      name:Apoptosis
      issn:
         1573-675X
         1360-8185
      volumeNumber:13
Organization:
      name:Springer US
      logo:
         url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
         type:ImageObject
      name:University “Roma Tre”
      address:
         name:Department of Biology, University “Roma Tre”, Rome, Italy
         type:PostalAddress
      name:University “Roma Tre”
      address:
         name:Department of Biology, University “Roma Tre”, Rome, Italy
         type:PostalAddress
      name:University “Roma Tre”
      address:
         name:Department of Biology, University “Roma Tre”, Rome, Italy
         type:PostalAddress
      name:SIGMA-TAU Industrie Farmaceutiche Riunite S.p.A
      address:
         name:SIGMA-TAU Industrie Farmaceutiche Riunite S.p.A, Pomezia, Italy
         type:PostalAddress
      name:Istituto Superiore di Sanità
      address:
         name:Department of Technology and Health, Istituto Superiore di Sanità, Rome, Italy
         type:PostalAddress
      name:Istituto Superiore di Sanità
      address:
         name:Department of Technology and Health, Istituto Superiore di Sanità, Rome, Italy
         type:PostalAddress
      name:Istituto Superiore di Sanità
      address:
         name:Department of Technology and Health, Istituto Superiore di Sanità, Rome, Italy
         type:PostalAddress
      name:University “Roma Tre”
      address:
         name:Department of Biology, University “Roma Tre”, Rome, Italy
         type:PostalAddress
ImageObject:
      url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
Person:
      name:Chiara Cenciarelli
      affiliation:
            name:University “Roma Tre”
            address:
               name:Department of Biology, University “Roma Tre”, Rome, Italy
               type:PostalAddress
            type:Organization
      name:Caterina Tanzarella
      affiliation:
            name:University “Roma Tre”
            address:
               name:Department of Biology, University “Roma Tre”, Rome, Italy
               type:PostalAddress
            type:Organization
      name:Ilio Vitale
      affiliation:
            name:University “Roma Tre”
            address:
               name:Department of Biology, University “Roma Tre”, Rome, Italy
               type:PostalAddress
            type:Organization
      name:Claudio Pisano
      affiliation:
            name:SIGMA-TAU Industrie Farmaceutiche Riunite S.p.A
            address:
               name:SIGMA-TAU Industrie Farmaceutiche Riunite S.p.A, Pomezia, Italy
               type:PostalAddress
            type:Organization
      name:Pasqualina Crateri
      affiliation:
            name:Istituto Superiore di Sanità
            address:
               name:Department of Technology and Health, Istituto Superiore di Sanità, Rome, Italy
               type:PostalAddress
            type:Organization
      name:Stefania Meschini
      affiliation:
            name:Istituto Superiore di Sanità
            address:
               name:Department of Technology and Health, Istituto Superiore di Sanità, Rome, Italy
               type:PostalAddress
            type:Organization
      name:Giuseppe Arancia
      affiliation:
            name:Istituto Superiore di Sanità
            address:
               name:Department of Technology and Health, Istituto Superiore di Sanità, Rome, Italy
               type:PostalAddress
            type:Organization
      name:Antonio Antoccia
      affiliation:
            name:University “Roma Tre”
            address:
               name:Department of Biology, University “Roma Tre”, Rome, Italy
               type:PostalAddress
            type:Organization
      email:[email protected]
PostalAddress:
      name:Department of Biology, University “Roma Tre”, Rome, Italy
      name:Department of Biology, University “Roma Tre”, Rome, Italy
      name:Department of Biology, University “Roma Tre”, Rome, Italy
      name:SIGMA-TAU Industrie Farmaceutiche Riunite S.p.A, Pomezia, Italy
      name:Department of Technology and Health, Istituto Superiore di Sanità, Rome, Italy
      name:Department of Technology and Health, Istituto Superiore di Sanità, Rome, Italy
      name:Department of Technology and Health, Istituto Superiore di Sanità, Rome, Italy
      name:Department of Biology, University “Roma Tre”, Rome, Italy
WebPageElement:
      isAccessibleForFree:
      cssSelector:.main-content

External Links {🔗}(239)

Analytics and Tracking {📊}

  • Google Tag Manager

Libraries {📚}

  • Clipboard.js
  • Prism.js

Emails and Hosting {✉️}

Mail Servers:

  • mx.zoho.eu
  • mx2.zoho.eu
  • mx3.zoho.eu

Name Servers:

  • josh.ns.cloudflare.com
  • zita.ns.cloudflare.com

CDN Services {📦}

  • Crossref

4.28s.