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We began analyzing https://link.springer.com/article/10.1007/s00428-012-1232-0, but it redirected us to https://link.springer.com/article/10.1007/s00428-012-1232-0. The analysis below is for the second page.

Title[redir]:
An improved image analysis method for cell counting lends credibility to the prognostic significance of T cells in colorectal cancer | Virchows Archiv
Description:
Numerous immunohistochemically detectable proteins, such as immune cell surface (CD) proteins, vascular endothelial growth factor, and matrix metalloproteinases, have been proposed as potential prognostic markers in colorectal cancer (CRC) and other malignancies. However, the lack of reproducibility has been a major problem in validating the clinical use of such markers, and this has been attributed to insufficiently robust methods used in immunohistochemical staining or its assessment. In this study, we assessed how computer-assisted image analysis might contribute to the reliable assessment of positive area percentage and immune cell density in CRC specimens, and subsequently, we applied the computer-assisted cell counting method in assessing the prognostic value of T cell infiltration in CRC. The computer-assisted analysis methods were based on separating hematoxylin and diaminobenzidine color layers and then applying a brightness threshold using open source image analysis software ImageJ. We found that computer-based analysis results in a more reproducible assessment of the immune positive area percentage than visual semiquantitative estimation. Computer-assisted immune cell counting was rapid to perform and accurate (Pearson r > 0.96 with exact manual cell counts). Moreover, the computer-assisted determination of peritumoral and stromal T cell density had independent prognostic value. Our results suggest that computer-assisted image analysis, utilizing freely available image analysis software, provides a valuable alternative to semiquantitative assessment of immunohistochemical results in cancer research, as well as in clinical practice. The advantages of using computer-assisted analysis include objectivity, accuracy, reproducibility, and time efficiency. This study supports the prognostic value of assessing T cell infiltration in CRC.

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Keywords {🔍}

cancer, article, google, scholar, pubmed, colorectal, cas, analysis, cell, image, prognostic, computerassisted, prognosis, immune, clin, assessment, infiltration, imagej, pathol, esm, cells, color, zlobec, lugli, res, jpeg, privacy, cookies, content, significance, tumor, colon, receptor, deconvolution, processing, publish, research, search, counting, juha, factor, crc, clinical, methods, immunohistochemical, study, semiquantitative, access, immunohistochemistry, breast,

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uk/landinig/software/cdeconv/cdeconv tumour-infiltrating t-cell subsets cd8+ lymphocytes/tumour-budding index month download article/chapter matrix metalloproteinase/tissue inhibitors computer-assisted image analysis computer-assisted analysis methods �pro-/anti-tumour’ approach computer-based analysis results pro-/anti-tumor factors computer-assisted determination image analysis software full article pdf sporadic colorectal cancers tumour host interaction quantitative image analysis privacy choices/manage cookies quantitative immunohistochemical analysis tumor-infiltrating lymphocytes tumor infiltrating lymphocytes positive area percentage t-cell infiltration vegf expression predict automated imaging systems budding invasive margin cell prognosis access human colorectal cancer immune cell surface european economic area cells cd4-positive cell receptor clonotype cancer cell nests related subjects beta-catenin expression dieu-nosjean mc colorectal cancer—relevance polyposis colorectal cancer finnish cancer foundation diaminobenzidine color layers emil aaltonen foundation activated egfr expression biomedical image processing human colon carcinomas conditions privacy policy electronic supplementary material color deconvolution plugin rectal cancer treated visual semiquantitative estimation selecting immunohistochemical cut immune cell density

Questions {❓}

Doger FK, Meteoglu I, Tuncyurek P, Okyay P, Cevikel H (2006) Does the EGFR and VEGF expression predict the prognosis in colon cancer?

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         description:Numerous immunohistochemically detectable proteins, such as immune cell surface (CD) proteins, vascular endothelial growth factor, and matrix metalloproteinases, have been proposed as potential prognostic markers in colorectal cancer (CRC) and other malignancies. However, the lack of reproducibility has been a major problem in validating the clinical use of such markers, and this has been attributed to insufficiently robust methods used in immunohistochemical staining or its assessment. In this study, we assessed how computer-assisted image analysis might contribute to the reliable assessment of positive area percentage and immune cell density in CRC specimens, and subsequently, we applied the computer-assisted cell counting method in assessing the prognostic value of T cell infiltration in CRC. The computer-assisted analysis methods were based on separating hematoxylin and diaminobenzidine color layers and then applying a brightness threshold using open source image analysis software ImageJ. We found that computer-based analysis results in a more reproducible assessment of the immune positive area percentage than visual semiquantitative estimation. Computer-assisted immune cell counting was rapid to perform and accurate (Pearson r > 0.96 with exact manual cell counts). Moreover, the computer-assisted determination of peritumoral and stromal T cell density had independent prognostic value. Our results suggest that computer-assisted image analysis, utilizing freely available image analysis software, provides a valuable alternative to semiquantitative assessment of immunohistochemical results in cancer research, as well as in clinical practice. The advantages of using computer-assisted analysis include objectivity, accuracy, reproducibility, and time efficiency. This study supports the prognostic value of assessing T cell infiltration in CRC.
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