
DOI . ORG {
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Title[redir]:
Immunocompetence of human microvascular brain endothelial cells: cytokine regulation of IL-1β, MCP-1, IL-10, sICAM-1 and sVCAM-1 | Journal of Neurology
Description:
Endothelia from the brains of four patients undergoing neurosurgery, including one multiple sclerosis (MS) patient, were studied in vitro to determine cytokine and chemokine production; the release of soluble adhesion molecules was also investigated. The same procedure was repeated on human umbilical vein endothelial cells (HUVECs) in order to detect possible district-specific differences. After isolation, the endothelium was cultured and stimulated with γ-interferon (IFN), tumour necrosis factor alpha (TNF-α) and LPS. The results showed that brain endothelium, in our experimental conditions, does not produce interleukin (IL)-10 and produces lower amounts of IL-1β and soluble intercellular adhesion molecule-1 (sICAM-1) than HUVECs do; no differences were detected in soluble vascular cell adhesion molecule-1 (sVCAM-1) production. MCP-1 mRNA was detected both without and after stimulation with TNF-α and γ-IFN in HUVECs and MS human brain endothelial cells (HBECs), while in non-MS-HBECs it was found only after γ-IFN stimulation.
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Keywords {🔍}
article, cells, access, brain, endothelial, privacy, cookies, content, journal, human, information, publish, search, ilβ, mcp, open, data, log, research, microvascular, cytokine, sicam, svcam, frigerio, gelati, ciusani, soluble, adhesion, huvecs, endothelium, tnfα, interleukin, discover, springer, optional, personal, including, parties, policy, find, track, neurology, immunocompetence, regulation, cite, corsini, dufour, massa, salmaggi, explore,
Topics {✒️}
month download article/chapter il-1β differentially regulate related subjects full article pdf privacy choices/manage cookies brain endothelium soluble adhesion molecules district-specific differences produce interleukin check access instant access european economic area scope submit manuscript patients undergoing neurosurgery produces lower amounts pro-trophic phenotype pro-inflammatory tnfα conditions privacy policy accepting optional cookies multiple sclerosis journal finder publish article journal article log γ-ifn stimulation article cite il-1β article frigerio privacy policy personal data mcp-1 mrna books a optional cookies manage preferences vitro inflammatory phenotype journal publish subscription content similar content data protection essential cookies cookies skip neurology aims experimental conditions institution subscribe γ-interferon γ-ifn anti-inflammatory endothelium information ms-hbecs
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headline:Immunocompetence of human microvascular brain endothelial cells: cytokine regulation of IL-1β, MCP-1, IL-10, sICAM-1 and sVCAM-1
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Neurosciences
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headline:Immunocompetence of human microvascular brain endothelial cells: cytokine regulation of IL-1β, MCP-1, IL-10, sICAM-1 and sVCAM-1
description: Endothelia from the brains of four patients undergoing neurosurgery, including one multiple sclerosis (MS) patient, were studied in vitro to determine cytokine and chemokine production; the release of soluble adhesion molecules was also investigated. The same procedure was repeated on human umbilical vein endothelial cells (HUVECs) in order to detect possible district-specific differences. After isolation, the endothelium was cultured and stimulated with γ-interferon (IFN), tumour necrosis factor alpha (TNF-α) and LPS. The results showed that brain endothelium, in our experimental conditions, does not produce interleukin (IL)-10 and produces lower amounts of IL-1β and soluble intercellular adhesion molecule-1 (sICAM-1) than HUVECs do; no differences were detected in soluble vascular cell adhesion molecule-1 (sVCAM-1) production. MCP-1 mRNA was detected both without and after stimulation with TNF-α and γ-IFN in HUVECs and MS human brain endothelial cells (HBECs), while in non-MS-HBECs it was found only after γ-IFN stimulation.
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Neurology
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