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Title[redir]:
Quantitative PCR assays for mouse enteric flora reveal strain-dependent differences in composition that are influenced by the microenvironment | Mammalian Genome
Description:
The mammalian gastrointestinal (GI) tract is inhabited by over a hundred species of symbiotic bacteria. Differences among individuals in the composition of the GI flora may contribute to variation in in vivo experimental analyses and disease susceptibility. To investigate potential interindividual differences in GI flora composition, we developed real-time quantitative PCR-based assays for the detection of the eight members of the Altered Schaedler Flora (ASF) as representative members of different bacterial niches within the mammalian GI tract. Quantitative and reproducible strain-specific variations in the numbers of the ASF members were observed across 23 different barrier-housed inbred mouse strains, suggesting that the ASF assays can be used as sentinels for changes in GI flora composition. A significant cage effect was also detected. Isogenic mice that cohabited at weaning, whether from the same or different litters, showed little variation in ASF profiles. Conversely, litters split among different cages at weaning showed divergence in ASF profiles after three weeks. Individual ASF profiles, once established, were highly stable over time in the absence of environmental perturbation. Furthermore, cohabitation of different inbred strains maintained most of the interstrain variation in the GI flora, supporting a role of host genetics in determining GI flora composition.
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Keywords {๐}
google, scholar, pubmed, article, cas, flora, mice, gastrointestinal, mouse, microbiol, composition, tract, access, effects, assays, orcutt, schaedler, asf, microbial, intestinal, privacy, cookies, content, social, search, bacteria, inbred, strains, genetics, behav, usa, gastroenterol, wahlsten, analysis, data, publish, research, mammalian, genome, quantitative, differences, variation, altered, bacterial, effect, environmental, open, stress, brain, small,
Topics {โ๏ธ}
irritable bowel syndrome month download article/chapter quantitative pcr assays reproducible strain-specific variations home cage activity significant cage effect 2-dimethylhydrazine-induced colon carcinogenesis small intestine full article pdf fecal flora tumor-preventing effect mouse-colonizing strains small intestinal motility defined murine microbiota privacy choices/manage cookies docile inbred strain related subjects savage dc inflammatory bowel disease crabbe jc check access clostridium difficile infection microbial ecology instant access mouse gastrointestinal tract mouse intestinal bacteria joseph baker jr mammalian gi tract nci gnotobiotic rodents inbred strains maintained european economic area scope submit manuscript vivo experimental analyses regulates fat storage heterogeneous metabolic adaptation high-fat diet bacteroides fragilis isolates major histocompatibility complex boehm sl 2nd dual-labeled probes altered schaedler flora ๏ฟฝaltered schaedler floraโ autochthonous bacterial flora nih grant dk34987 nih grant ca79869 nih grant ca84239 mamm genome 17 conditions privacy policy vasoactive intestinal polypeptide predominant bacterial populations
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headline:Quantitative PCR assays for mouse enteric flora reveal strain-dependent differences in composition that are influenced by the microenvironment
description:The mammalian gastrointestinal (GI) tract is inhabited by over a hundred species of symbiotic bacteria. Differences among individuals in the composition of the GI flora may contribute to variation in in vivo experimental analyses and disease susceptibility. To investigate potential interindividual differences in GI flora composition, we developed real-time quantitative PCR-based assays for the detection of the eight members of the Altered Schaedler Flora (ASF) as representative members of different bacterial niches within the mammalian GI tract. Quantitative and reproducible strain-specific variations in the numbers of the ASF members were observed across 23 different barrier-housed inbred mouse strains, suggesting that the ASF assays can be used as sentinels for changes in GI flora composition. A significant cage effect was also detected. Isogenic mice that cohabited at weaning, whether from the same or different litters, showed little variation in ASF profiles. Conversely, litters split among different cages at weaning showed divergence in ASF profiles after three weeks. Individual ASF profiles, once established, were highly stable over time in the absence of environmental perturbation. Furthermore, cohabitation of different inbred strains maintained most of the interstrain variation in the GI flora, supporting a role of host genetics in determining GI flora composition.
datePublished:2006-11-07T00:00:00Z
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Fecal Sample
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Small Intestine Transit
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headline:Quantitative PCR assays for mouse enteric flora reveal strain-dependent differences in composition that are influenced by the microenvironment
description:The mammalian gastrointestinal (GI) tract is inhabited by over a hundred species of symbiotic bacteria. Differences among individuals in the composition of the GI flora may contribute to variation in in vivo experimental analyses and disease susceptibility. To investigate potential interindividual differences in GI flora composition, we developed real-time quantitative PCR-based assays for the detection of the eight members of the Altered Schaedler Flora (ASF) as representative members of different bacterial niches within the mammalian GI tract. Quantitative and reproducible strain-specific variations in the numbers of the ASF members were observed across 23 different barrier-housed inbred mouse strains, suggesting that the ASF assays can be used as sentinels for changes in GI flora composition. A significant cage effect was also detected. Isogenic mice that cohabited at weaning, whether from the same or different litters, showed little variation in ASF profiles. Conversely, litters split among different cages at weaning showed divergence in ASF profiles after three weeks. Individual ASF profiles, once established, were highly stable over time in the absence of environmental perturbation. Furthermore, cohabitation of different inbred strains maintained most of the interstrain variation in the GI flora, supporting a role of host genetics in determining GI flora composition.
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