DOI . ORG {}

Matching Content Categories {📚}

• Telecommunications
• Science
• Mobile Technology & AI

Content Management System {📝}

What CMS is doi.org built with?

Custom-built

No common CMS systems were detected on Doi.org, and no known web development framework was identified.

Traffic Estimate {📈}

What is the average monthly size of doi.org audience?

🌠 Phenomenal Traffic: 5M - 10M visitors per month


Based on our best estimate, this website will receive around 5,000,019 visitors per month in the current month.
However, some sources were not loaded, we suggest to reload the page to get complete results.

check SE Ranking
check Ahrefs
check Similarweb
check Ubersuggest
check Semrush

How Does Doi.org Make Money? {💸}

We find it hard to spot revenue streams.

Not all websites focus on profit; some are designed to educate, connect people, or share useful tools. People create websites for numerous reasons. And this could be one such example. Doi.org could be secretly minting cash, but we can't detect the process.

Keywords {🔍}

article, channels, cell, membrane, migration, oscillations, cells, ach, calcium, privacy, cookies, content, journal, glioma, access, information, publish, search, muscarinic, activation, inhibition, intracellular, ion, data, log, research, bordey, sontheimer, trouslard, muscarine, discover, springer, optional, personal, parties, policy, find, track, biology, induces, leads, cite, explore, acetylcholine, human, astrocytoma, perforated, patchclamp, application, abolished,

Topics {✒️}

intracellular arachidonate-activated channels perforated patch-clamp recording month download article/chapter turn activate ca2+-dependent privacy choices/manage cookies membrane biology aims full article pdf periodic ca2+ oscillations astrocytoma cells exhibited glioma cells induce glioma cell migration european economic area scope submit manuscript investigate physiological effects long tail region early transient rise related subjects cell migration published conditions privacy policy cell moved forward accepting optional cookies ach receptor agonist prolonged ach applications main content log check access instant access 17 december/revised bk-type ach-induced [ca2+] calcium microspectrofluorimetry external calcium + channels journal finder publish glioma cells voltage-dependent cell migration membrane ruffling membrane biol article log privacy policy personal data books a intracellular injection intracellular stores article cite optional cookies article bordey manage preferences muscarinic activation muscarinic antagonists

Schema {🗺️}

WebPage:
      mainEntity:
         headline:Muscarinic Activation of BK Channels Induces Membrane Oscillations in Glioma Cells and Leads to Inhibition of Cell Migration
         description: Patients with cerebral tumors often present with elevated levels of acetylcholine (ACh) in their cerebrospinal fluid. This motivated us to investigate physiological effects of ACh on cultured human astrocytoma cells (U373) using a combination of videomicroscopy, calcium microspectrofluorimetry and perforated patch-clamp recording. Astrocytoma cells exhibited the typical morphological changes associated with cell migration; polarized cells displayed prominent lamellipodia and associated membrane ruffling at the anterior of the cell, and a long tail region that periodically contracted into the cell body as the cell moved forward. Bath application of the ACh receptor agonist, muscarine, reversibly inhibited cell migration. In conjunction with this inhibition, ACh induced a dose-dependent, biphasic increase in resting intracellular free calcium concentration ([Ca2+] i ) associated with periodic Ca2+ oscillations during prolonged ACh applications. The early transient rise in [Ca2+] i was abolished by ionomycin and thapsigargin but was insensitive to caffeine and ryanodine while the plateau phase was strictly dependent on external calcium. The Ca2+ response to ACh was mimicked by muscarine and abolished by the muscarinic antagonists, atropine or 4-DAMP, but not by pirenzepine. Using perforated patch-clamp recordings combined with fluorescent imaging, we demonstrated that ACh-induced [Ca2+] i oscillations triggered membrane voltage oscillations that were due to the activation of voltage-dependent, Ca2+-sensitive K+ currents. These K+ currents were blocked by intracellular injection of EGTA, or by extracellular application of TEA, quinine, or charybdotoxin, but not by apamin. These studies suggest that activation of muscarinic receptors on glioma cells induce the release of Ca2+ from intracellular stores which in turn activate Ca2+-dependent (BK-type) K+ channels. Furthermore, this effect was associated with inhibition of cell migration, suggesting an interaction of this pathway with glioma cell migration.
         datePublished:
         dateModified:
         pageStart:31
         pageEnd:40
         sameAs:https://doi.org/10.1007/s00232001073
         keywords:
            Key words: BK channels — Muscarine — Acetylcholine — Calcium — Migration — Tumor
            Biochemistry
            general
            Human Physiology
         image:
         isPartOf:
            name:The Journal of Membrane Biology
            issn:
               1432-1424
               0022-2631
            volumeNumber:176
            type:
               Periodical
               PublicationVolume
         publisher:
            name:Springer-Verlag
            logo:
               url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
               type:ImageObject
            type:Organization
         author:
               name:A. Bordey
               affiliation:
                     name:Department of Neurobiology, University of Alabama at Birmingham, Birmingham, AL 35294 USA
                     address:
                        name:Department of Neurobiology, University of Alabama at Birmingham, Birmingham, AL 35294 USA, , US
                        type:PostalAddress
                     type:Organization
               type:Person
               name:H. Sontheimer
               affiliation:
                     name:Department of Neurobiology, University of Alabama at Birmingham, Birmingham, AL 35294 USA
                     address:
                        name:Department of Neurobiology, University of Alabama at Birmingham, Birmingham, AL 35294 USA, , US
                        type:PostalAddress
                     type:Organization
               type:Person
               name:J. Trouslard
               affiliation:
                     name:Institut de Physiologie URA 7519, 21 rue R. Descartes, 67084 Strasbourg Cedex France
                     address:
                        name:Institut de Physiologie URA 7519, 21 rue R. Descartes, 67084 Strasbourg Cedex France, , FR
                        type:PostalAddress
                     type:Organization
               type:Person
         isAccessibleForFree:
         hasPart:
            isAccessibleForFree:
            cssSelector:.main-content
            type:WebPageElement
         type:ScholarlyArticle
      context:https://schema.org
ScholarlyArticle:
      headline:Muscarinic Activation of BK Channels Induces Membrane Oscillations in Glioma Cells and Leads to Inhibition of Cell Migration
      description: Patients with cerebral tumors often present with elevated levels of acetylcholine (ACh) in their cerebrospinal fluid. This motivated us to investigate physiological effects of ACh on cultured human astrocytoma cells (U373) using a combination of videomicroscopy, calcium microspectrofluorimetry and perforated patch-clamp recording. Astrocytoma cells exhibited the typical morphological changes associated with cell migration; polarized cells displayed prominent lamellipodia and associated membrane ruffling at the anterior of the cell, and a long tail region that periodically contracted into the cell body as the cell moved forward. Bath application of the ACh receptor agonist, muscarine, reversibly inhibited cell migration. In conjunction with this inhibition, ACh induced a dose-dependent, biphasic increase in resting intracellular free calcium concentration ([Ca2+] i ) associated with periodic Ca2+ oscillations during prolonged ACh applications. The early transient rise in [Ca2+] i was abolished by ionomycin and thapsigargin but was insensitive to caffeine and ryanodine while the plateau phase was strictly dependent on external calcium. The Ca2+ response to ACh was mimicked by muscarine and abolished by the muscarinic antagonists, atropine or 4-DAMP, but not by pirenzepine. Using perforated patch-clamp recordings combined with fluorescent imaging, we demonstrated that ACh-induced [Ca2+] i oscillations triggered membrane voltage oscillations that were due to the activation of voltage-dependent, Ca2+-sensitive K+ currents. These K+ currents were blocked by intracellular injection of EGTA, or by extracellular application of TEA, quinine, or charybdotoxin, but not by apamin. These studies suggest that activation of muscarinic receptors on glioma cells induce the release of Ca2+ from intracellular stores which in turn activate Ca2+-dependent (BK-type) K+ channels. Furthermore, this effect was associated with inhibition of cell migration, suggesting an interaction of this pathway with glioma cell migration.
      datePublished:
      dateModified:
      pageStart:31
      pageEnd:40
      sameAs:https://doi.org/10.1007/s00232001073
      keywords:
         Key words: BK channels — Muscarine — Acetylcholine — Calcium — Migration — Tumor
         Biochemistry
         general
         Human Physiology
      image:
      isPartOf:
         name:The Journal of Membrane Biology
         issn:
            1432-1424
            0022-2631
         volumeNumber:176
         type:
            Periodical
            PublicationVolume
      publisher:
         name:Springer-Verlag
         logo:
            url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
            type:ImageObject
         type:Organization
      author:
            name:A. Bordey
            affiliation:
                  name:Department of Neurobiology, University of Alabama at Birmingham, Birmingham, AL 35294 USA
                  address:
                     name:Department of Neurobiology, University of Alabama at Birmingham, Birmingham, AL 35294 USA, , US
                     type:PostalAddress
                  type:Organization
            type:Person
            name:H. Sontheimer
            affiliation:
                  name:Department of Neurobiology, University of Alabama at Birmingham, Birmingham, AL 35294 USA
                  address:
                     name:Department of Neurobiology, University of Alabama at Birmingham, Birmingham, AL 35294 USA, , US
                     type:PostalAddress
                  type:Organization
            type:Person
            name:J. Trouslard
            affiliation:
                  name:Institut de Physiologie URA 7519, 21 rue R. Descartes, 67084 Strasbourg Cedex France
                  address:
                     name:Institut de Physiologie URA 7519, 21 rue R. Descartes, 67084 Strasbourg Cedex France, , FR
                     type:PostalAddress
                  type:Organization
            type:Person
      isAccessibleForFree:
      hasPart:
         isAccessibleForFree:
         cssSelector:.main-content
         type:WebPageElement
["Periodical","PublicationVolume"]:
      name:The Journal of Membrane Biology
      issn:
         1432-1424
         0022-2631
      volumeNumber:176
Organization:
      name:Springer-Verlag
      logo:
         url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
         type:ImageObject
      name:Department of Neurobiology, University of Alabama at Birmingham, Birmingham, AL 35294 USA
      address:
         name:Department of Neurobiology, University of Alabama at Birmingham, Birmingham, AL 35294 USA, , US
         type:PostalAddress
      name:Department of Neurobiology, University of Alabama at Birmingham, Birmingham, AL 35294 USA
      address:
         name:Department of Neurobiology, University of Alabama at Birmingham, Birmingham, AL 35294 USA, , US
         type:PostalAddress
      name:Institut de Physiologie URA 7519, 21 rue R. Descartes, 67084 Strasbourg Cedex France
      address:
         name:Institut de Physiologie URA 7519, 21 rue R. Descartes, 67084 Strasbourg Cedex France, , FR
         type:PostalAddress
ImageObject:
      url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
Person:
      name:A. Bordey
      affiliation:
            name:Department of Neurobiology, University of Alabama at Birmingham, Birmingham, AL 35294 USA
            address:
               name:Department of Neurobiology, University of Alabama at Birmingham, Birmingham, AL 35294 USA, , US
               type:PostalAddress
            type:Organization
      name:H. Sontheimer
      affiliation:
            name:Department of Neurobiology, University of Alabama at Birmingham, Birmingham, AL 35294 USA
            address:
               name:Department of Neurobiology, University of Alabama at Birmingham, Birmingham, AL 35294 USA, , US
               type:PostalAddress
            type:Organization
      name:J. Trouslard
      affiliation:
            name:Institut de Physiologie URA 7519, 21 rue R. Descartes, 67084 Strasbourg Cedex France
            address:
               name:Institut de Physiologie URA 7519, 21 rue R. Descartes, 67084 Strasbourg Cedex France, , FR
               type:PostalAddress
            type:Organization
PostalAddress:
      name:Department of Neurobiology, University of Alabama at Birmingham, Birmingham, AL 35294 USA, , US
      name:Department of Neurobiology, University of Alabama at Birmingham, Birmingham, AL 35294 USA, , US
      name:Institut de Physiologie URA 7519, 21 rue R. Descartes, 67084 Strasbourg Cedex France, , FR
WebPageElement:
      isAccessibleForFree:
      cssSelector:.main-content

External Links {🔗}(67)

• What's the financial gain of https://link.springer.com/privacystatement?
• What's the income generated by https://link.springer.com/article/10.1007/s00232001073/#main each month?
• Learn about the earnings of https://link.springer.com
• How much does https://idp.springer.com/auth/personal/springernature?redirect_uri=https://link.springer.com/article/10.1007/s00232001073 pull in?
• Learn how profitable https://link.springer.com/article/10.1007/s00232001073/#eds-c-header-nav is on a monthly basis
• What is the monthly revenue of https://link.springer.com/journals/?
• https://www.springernature.com/gp/authors's revenue stream
• Earnings of https://link.springernature.com/home/
• How much money does https://link.springer.com/article/10.1007/s00232001073/#eds-c-header-popup-search generate?
• How much does https://order.springer.com/public/cart pull in?
• How much income does https://link.springer.com/article/10.1007/s00232001073/ have?
• How much does https://link.springer.com/article/10.1007/s00232001073/journal/232 gross monthly?
• What is the monthly revenue of https://link.springer.com/article/10.1007/s00232001073/#citeas?
• Earnings of https://link.springer.com/article/10.1007/s00232001073/journal/232/aims-and-scope
• What are the total earnings of https://submission.nature.com/new-submission/232/3?
• Learn how profitable https://link.springer.com/article/10.1007/s00232001073/#auth-A__-Bordey-A1 is on a monthly basis
• How much does https://link.springer.com/article/10.1007/s00232001073/#A1 pull in?
• Get to know what's the income of https://link.springer.com/article/10.1007/s00232001073/#auth-H__-Sontheimer-A1
• How much profit does https://link.springer.com/article/10.1007/s00232001073/#auth-J__-Trouslard-A2 generate?
• Financial intake of https://link.springer.com/article/10.1007/s00232001073/#A2
• What's the total monthly financial gain of https://link.springer.com/article/10.1007/s00232001073/article/10.1007/s00232001073/metrics?
• What's the income generated by https://wayf.springernature.com?redirect_uri=https%3A%2F%2Flink.springer.com%2Farticle%2F10.1007%2Fs00232001073 each month?
• What's the monthly money flow for https://link.springer.com/product/springer-plus?
• Get to know what's the income of https://www.springernature.com/gp/librarians/licensing/agc/journals
• Get to know https://link.springer.com/10.1007/s11010-019-03504-z?fromPaywallRec=true's earnings
• How profitable is https://link.springer.com/10.1007/s00204-020-02956-3?fromPaywallRec=true?
• What's the monthly money flow for https://link.springer.com/10.1007/112_2020_44?fromPaywallRec=true?
• Monthly income for https://link.springer.com/article/10.1007/s00232001073/subjects/calcium-signalling
• Find out how much https://link.springer.com/article/10.1007/s00232001073/subjects/calcium-channels earns monthly
• What's the profit of https://link.springer.com/article/10.1007/s00232001073/subjects/ion-channels-in-the-nervous-system?
• How much profit is https://link.springer.com/article/10.1007/s00232001073/subjects/ion-channels making per month?
• What's the financial intake of https://link.springer.com/article/10.1007/s00232001073/subjects/potassium-channels?
• How much revenue does https://link.springer.com/article/10.1007/s00232001073/subjects/ion-channel-signalling bring in?
• How much does https://link.springer.com/article/10.1007/s00232001073/search?sortBy=newestFirst&dc.creator=A.%20%20Bordey net monthly?
• Explore the financials of https://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=search&term=A.%20%20Bordey
• What's https://scholar.google.co.uk/scholar?as_q=&num=10&btnG=Search+Scholar&as_epq=&as_oq=&as_eq=&as_occt=any&as_sauthors=%22A.%20%20Bordey%22&as_publication=&as_ylo=&as_yhi=&as_allsubj=all&hl=en's gross income?
• Income figures for https://link.springer.com/article/10.1007/s00232001073/search?sortBy=newestFirst&dc.creator=H.%20%20Sontheimer
• https://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=search&term=H.%20%20Sontheimer income
• How much profit does https://scholar.google.co.uk/scholar?as_q=&num=10&btnG=Search+Scholar&as_epq=&as_oq=&as_eq=&as_occt=any&as_sauthors=%22H.%20%20Sontheimer%22&as_publication=&as_ylo=&as_yhi=&as_allsubj=all&hl=en make?
• What's the profit of https://link.springer.com/article/10.1007/s00232001073/search?sortBy=newestFirst&dc.creator=J.%20%20Trouslard?
• Find out how much https://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=search&term=J.%20%20Trouslard earns monthly
• How much does https://scholar.google.co.uk/scholar?as_q=&num=10&btnG=Search+Scholar&as_epq=&as_oq=&as_eq=&as_occt=any&as_sauthors=%22J.%20%20Trouslard%22&as_publication=&as_ylo=&as_yhi=&as_allsubj=all&hl=en bring in each month?
• Learn about the earnings of https://s100.copyright.com/AppDispatchServlet?title=Muscarinic%20Activation%20of%20BK%20Channels%20Induces%20Membrane%20Oscillations%20in%20Glioma%20Cells%20and%20Leads%20to%20Inhibition%20of%20Cell%20Migration&author=A.%20%20Bordey%20et%20al&contentID=10.1007%2Fs00232001073©right=Springer-Verlag%20New%20York%20Inc.&publication=0022-2631&publicationDate=2000-07&publisherName=SpringerNature&orderBeanReset=true
• Check the income stats for https://citation-needed.springer.com/v2/references/10.1007/s00232001073?format=refman&flavour=citation
• How much does https://link.springer.com/article/10.1007/s00232001073/search?query=Key%20words%3A%20BK%20channels%20%E2%80%94%20Muscarine%20%E2%80%94%20Acetylcholine%20%E2%80%94%20Calcium%20%E2%80%94%20Migration%20%E2%80%94%20Tumor&facet-discipline="Life%20Sciences" net monthly?
• How much does https://link.springer.com/journals/a/1 pull in monthly?
• How much cash flow does https://link.springer.com/books/a/1 have monthly?
• What's the total monthly financial gain of https://link.springer.com/journals?
• What's the income generated by https://authorservices.springernature.com/go/sn/?utm_source=SNLinkfooter&utm_medium=Web&utm_campaign=SNReferral each month?
• What's the income of https://www.springernature.com/gp/open-research/about/the-fundamentals-of-open-access-and-open-research?
• How much does https://www.springernature.com/gp/products pull in monthly?
• How much does https://www.springernature.com/gp/librarians net monthly?
• What are the total earnings of https://www.springernature.com/gp/societies?
• How much money does https://www.springernature.com/gp/partners generate?
• How much does https://www.springer.com/ gross monthly?
• What's the financial outcome of https://www.nature.com/?
• How much does https://www.biomedcentral.com/ bring in each month?
• Revenue of https://www.palgrave.com/
• How much does https://www.apress.com/ gross monthly?
• How much income does https://link.springer.com/brands/discover have?
• What's the financial outcome of https://www.springernature.com/gp/legal/ccpa?
• What are the earnings of https://www.springernature.com/gp/info/accessibility?
• What's the monthly money flow for https://link.springer.com/termsandconditions?
• How much does https://support.springernature.com/en/support/home earn?
• Explore the financials of https://link.springer.com/legal-notice
• Find out how much https://support.springernature.com/en/support/solutions/articles/6000255911-subscription-cancellations earns monthly
• Get to know what's the income of https://www.springernature.com/

Analytics and Tracking {📊}

• Google Tag Manager

Libraries {📚}

• Clipboard.js
• Prism.js

Emails and Hosting {✉️}

Mail Servers:

• mx.zoho.eu
• mx2.zoho.eu
• mx3.zoho.eu

Name Servers:

• josh.ns.cloudflare.com
• zita.ns.cloudflare.com

CDN Services {📦}

• Crossref