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DOI . ORG {}

  1. Analyzed Page
  2. Matching Content Categories
  3. CMS
  4. Monthly Traffic Estimate
  5. How Does Doi.org Make Money
  6. Keywords
  7. Topics
  8. Schema
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We began analyzing https://link.springer.com/article/10.1007/s00125-005-0105-3, but it redirected us to https://link.springer.com/article/10.1007/s00125-005-0105-3. The analysis below is for the second page.

Title[redir]:
Evidence that oestrogen receptor-α plays an important role in the regulation of glucose homeostasis in mice: insulin sensitivity in the liver | Diabetologia
Description:
Aims/hypothesis We used oestrogen receptor-α (ERα) knockout (ERKO) and receptor-β (ERβ) knockout (BERKO) mice to investigate the mechanism(s) behind the effects of oestrogens on glucose homeostasis. Methods Endogenous glucose production (EGP) was measured in ERKO mice using a euglycaemic–hyperinsulinaemic clamp. Insulin secretion was determined from isolated islets. In isolated muscles, glucose uptake was assayed by using radiolabelled isotopes. Genome-wide expression profiles were analysed by high-density oligonucleotide microarray assay, and the expression of the genes encoding steroyl-CoA desaturase and the Leptin receptor (Scd1 and Lepr, respectively) was confirmed by RT-PCR. Results ERKO mice had higher fasting blood glucose, plasma insulin levels and IGT. The plasma leptin level was increased, while the adiponectin concentration was decreased in ERKO mice. Levels of both glucose- and arginine-induced insulin secretion from isolated islets were similar in ERKO and wild-type mice. The euglycaemic–hyperinsulinaemic clamp revealed that suppression of EGP by increased insulin levels was blunted in ERKO mice, which suggests a pronounced hepatic insulin resistance. Microarray analysis revealed that in ERKO mice, the genes involved in hepatic lipid biosynthesis were upregulated, while genes involved in lipid transport were downregulated. Notably, hepatic Lepr expression was decreased in ERKO mice. In vitro studies showed a modest decrease in insulin-mediated glucose uptake in soleus and extensor digitorum longus (EDL) muscles of ERKO mice. BERKO mice demonstrated normal glucose tolerance and insulin release. Conclusions/interpretation We conclude that oestrogens, acting via ERα, regulate glucose homeostasis mainly by modulating hepatic insulin sensitivity, which can be due to the upregulation of lipogenic genes via the suppression of Lepr expression.

Matching Content Categories {📚}

  • Education
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Content Management System {📝}

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Custom-built

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🌠 Phenomenal Traffic: 5M - 10M visitors per month


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Keywords {🔍}

mice, insulin, glucose, erko, google, scholar, article, pubmed, cas, expression, levels, hepatic, genes, wildtype, resistance, increased, female, decreased, adiponectin, mouse, gene, leptin, plasma, muscle, sensitivity, min, role, scd, lepr, involved, transport, diabetes, categories, significantly, oestrogen, islets, uptake, metabolism, resistin, estrogen, data, liver, secretion, lipid, rate, table, homeostasis, berko, egp, isolated,

Topics {✒️}

semi-quantitative real-time rt-pcr liver-specific glucocorticoid-inducible genes glucose-induced full activation modify g-protein receptor table 1 euglycaemic–hyperinsulinaemic clamp oestrogen receptor-α plays diabetic goto–kakizaki rats hypothalamic–pituitary–gonadal axis genome-wide expression profiles goat anti-guinea pig euglycaemic–hyperinsulinaemic clamp revealed insulin-stimulated glucose uptake stearoyl-coa desaturase stearoyl-coa desaturase 1 stearoyl-coa desaturase-1 stearoyl coa desaturase saturated/unsaturated fatty acids endocrine sex reversal 125i-labelled murine adiponectin leptin-mediated weight loss long-chain fatty acids 125i-labeled murine resistin reduced hepatic glucose-6-phosphatase double-antibody ria techniques insulin-dependent diabetes mellitus real-time pcr insulin-resistant mouse models wild-type mouse livers web-based tool ‘htgm obesity-induced insulin resistance insulin-mediated glucose uptake double-antibody ria kit oestrogen-signalling pathway multiple-comparison correction based estrogen receptor gene euglycaemic–hyperinsulinaemic clamp oestrogen receptor-α pancreatic beta cells arginine-induced insulin secretion table 2 plasma leptin specific gene product arginine-induced insulin release wild-type mouse islets insulin resistance developed improved insulin sensitivity stimulated insulin release liver article published β-cell dysfunction privacy choices/manage cookies normal body weight

Schema {🗺️}

WebPage:
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         headline:Evidence that oestrogen receptor-α plays an important role in the regulation of glucose homeostasis in mice: insulin sensitivity in the liver
         description:We used oestrogen receptor-α (ERα) knockout (ERKO) and receptor-β (ERβ) knockout (BERKO) mice to investigate the mechanism(s) behind the effects of oestrogens on glucose homeostasis. Endogenous glucose production (EGP) was measured in ERKO mice using a euglycaemic–hyperinsulinaemic clamp. Insulin secretion was determined from isolated islets. In isolated muscles, glucose uptake was assayed by using radiolabelled isotopes. Genome-wide expression profiles were analysed by high-density oligonucleotide microarray assay, and the expression of the genes encoding steroyl-CoA desaturase and the Leptin receptor (Scd1 and Lepr, respectively) was confirmed by RT-PCR. ERKO mice had higher fasting blood glucose, plasma insulin levels and IGT. The plasma leptin level was increased, while the adiponectin concentration was decreased in ERKO mice. Levels of both glucose- and arginine-induced insulin secretion from isolated islets were similar in ERKO and wild-type mice. The euglycaemic–hyperinsulinaemic clamp revealed that suppression of EGP by increased insulin levels was blunted in ERKO mice, which suggests a pronounced hepatic insulin resistance. Microarray analysis revealed that in ERKO mice, the genes involved in hepatic lipid biosynthesis were upregulated, while genes involved in lipid transport were downregulated. Notably, hepatic Lepr expression was decreased in ERKO mice. In vitro studies showed a modest decrease in insulin-mediated glucose uptake in soleus and extensor digitorum longus (EDL) muscles of ERKO mice. BERKO mice demonstrated normal glucose tolerance and insulin release. We conclude that oestrogens, acting via ERα, regulate glucose homeostasis mainly by modulating hepatic insulin sensitivity, which can be due to the upregulation of lipogenic genes via the suppression of Lepr expression.
         datePublished:2006-02-04T00:00:00Z
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            Glucose homeostasis
            Insulin resistance
            Leptin receptor
            Stearoyl-CoA desaturase 1
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            Metabolic Diseases
            Human Physiology
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      headline:Evidence that oestrogen receptor-α plays an important role in the regulation of glucose homeostasis in mice: insulin sensitivity in the liver
      description:We used oestrogen receptor-α (ERα) knockout (ERKO) and receptor-β (ERβ) knockout (BERKO) mice to investigate the mechanism(s) behind the effects of oestrogens on glucose homeostasis. Endogenous glucose production (EGP) was measured in ERKO mice using a euglycaemic–hyperinsulinaemic clamp. Insulin secretion was determined from isolated islets. In isolated muscles, glucose uptake was assayed by using radiolabelled isotopes. Genome-wide expression profiles were analysed by high-density oligonucleotide microarray assay, and the expression of the genes encoding steroyl-CoA desaturase and the Leptin receptor (Scd1 and Lepr, respectively) was confirmed by RT-PCR. ERKO mice had higher fasting blood glucose, plasma insulin levels and IGT. The plasma leptin level was increased, while the adiponectin concentration was decreased in ERKO mice. Levels of both glucose- and arginine-induced insulin secretion from isolated islets were similar in ERKO and wild-type mice. The euglycaemic–hyperinsulinaemic clamp revealed that suppression of EGP by increased insulin levels was blunted in ERKO mice, which suggests a pronounced hepatic insulin resistance. Microarray analysis revealed that in ERKO mice, the genes involved in hepatic lipid biosynthesis were upregulated, while genes involved in lipid transport were downregulated. Notably, hepatic Lepr expression was decreased in ERKO mice. In vitro studies showed a modest decrease in insulin-mediated glucose uptake in soleus and extensor digitorum longus (EDL) muscles of ERKO mice. BERKO mice demonstrated normal glucose tolerance and insulin release. We conclude that oestrogens, acting via ERα, regulate glucose homeostasis mainly by modulating hepatic insulin sensitivity, which can be due to the upregulation of lipogenic genes via the suppression of Lepr expression.
      datePublished:2006-02-04T00:00:00Z
      dateModified:2006-02-04T00:00:00Z
      pageStart:588
      pageEnd:597
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      keywords:
         Estrogen receptors
         Glucose homeostasis
         Insulin resistance
         Leptin receptor
         Stearoyl-CoA desaturase 1
         Internal Medicine
         Metabolic Diseases
         Human Physiology
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      name:Department of Molecular Medicine, L6B:01 (Endocrinology), Karolinska Hospital, Stockholm, Sweden
      name:Department of Biosciences, Karolinska Institute, Novum, Sweden
      name:Department of Medicine, Lund University, Lund, Sweden
      name:Department of Surgical Sciences and Department of Physiology and Pharmacology, Karolinska Institute, Stockholm, Sweden
      name:Department of Surgical Sciences and Department of Physiology and Pharmacology, Karolinska Institute, Stockholm, Sweden
      name:Department of Surgical Sciences and Department of Physiology and Pharmacology, Karolinska Institute, Stockholm, Sweden
      name:Department of Biosciences, Karolinska Institute, Novum, Sweden
      name:KaroBio, Novum, Sweden
      name:Department of Medical Nutrition, Karolinska Institute, Novum, Sweden
      name:Department of Molecular Medicine, Karolinska Hospital, Karolinska Institute, Stockholm, Sweden
      name:Department of Molecular Medicine, Karolinska Hospital, Karolinska Institute, Stockholm, Sweden

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