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We began analyzing https://link.springer.com/article/10.1007/BF02789331, but it redirected us to https://link.springer.com/article/10.1007/BF02789331. The analysis below is for the second page.

Title[redir]:
Methods for construction of adenovirus vectors | Molecular Biotechnology
Description:
Adenoviruses are attracting increasing attention as general purpose mammalian cell expression vectors, as recombinant vaccines, and potentially as vectors for gene therapy. Not only is the adenovirus genome relatively easy to manipulate by recombinant DNA techniques, but adenovirus vectors are relatively stable, grow to high titers, and can transduce a variety of cell types in cell culture and in vivo. Vectors can be designed that are either replication competent or replication defective and, in the latter case, are highly efficient at delivering and expressing genes in mammalian cells without resulting in cell killing. Methods are described for growing, titrating, and purifying adenoviruses, for extracting viral DNA from purified virions and from infected cells, for rescuing inserts of foreign DNA into the viral genome, and for assessing expression of inserted genes in adenovirus vectors.

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Keywords {🔍}

adenovirus, google, scholar, article, pubmed, cas, graham, vectors, human, prevec, expression, gene, type, cell, virol, dna, privacy, cookies, content, access, virus, publish, search, construction, adenoviruses, recombinant, genome, gen, herpes, simplex, data, information, log, journal, research, molecular, methods, therapy, cells, viral, chapter, discover, genetic, hajahmad, bett, vector, usa, johnson, infectious, based,

Topics {✒️}

month download article/chapter gene therapy adenovirus gene expression firefly luciferase gene extracting viral dna related subjects full article pdf infectious adenovirus vectors privacy choices/manage cookies replication competent human adenovirus capsid human adenovirus dna virus surface antigen recombinant vaccines dna-protein complex ghosh-choudhury european economic area attracting increasing attention full-length genomes covalently closed circles van der eb check access instant access replication defective conditions privacy policy adenovirus vectors nucleic acids res accepting optional cookies rat embryo cells recombinant dna techniques viral genome infectious bacterial plasmids article graham main content log adenovirus type 5 adenovirus type 2 journal finder publish haj-ahmad article log virus res adenovirus vector recombinant adenovirus article cite gene 50 molecular genetics cell line purifying adenoviruses springer-verlag mammalian cells transfer

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