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Title[redir]:
Flow Cytometric Monitoring of Residual Disease in Acute Leukemia | SpringerLink
Description:
Multiparameter flow cytometry offers the unique ability to simultaneously assess and correlate multiple cellular properties at the single cell level in a timely and efficient manner. Application of this technique to the detection of residual acute leukemia after...
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Keywords {π}
leukemia, acute, google, scholar, residual, disease, pubmed, flow, cytometry, article, minimal, lymphoblastic, detection, blood, cas, protocol, myeloid, group, information, clin, wood, childhood, study, privacy, cookies, content, publish, cytometric, clinical, relapse, cytom, analysis, data, search, monitoring, methods, multiparameter, cell, prognostic, chapter, borowitz, implications, childrens, oncology, patients, risk, download, springer, usd, personal,
Topics {βοΈ}
month download article/chapter san-miguel jf flow cytometric monitoring fcm-mrd-study group flow cytometric follow acute lymphoblastic leukemia multiparameter flow cytometry privacy choices/manage cookies acute leukemia protocol acute myeloid leukemia color flow cytometry minimal residual disease residual acute leukemia hematological malignancies single cell level flow cytometry immunophentoyping device instant download abnormal hematopoietic cells residual disease detection oncology group study basic protocol outlines borowitz mj cell surface antigens european economic area monoclonal antibodies directed vidriales mb early immunophenotypical evaluation patient risk groups postinduction treatment stratification mouse igg2 antibodies provide prognostic information journal finder publish al i-bfm conditions privacy policy limited antibody panel del principe mi protocol wood accepting optional cookies relapse risk based mrd monitoring main content log flow cytometry acute leukemia coustan-smith protocol usdΒ 49 residual disease leukemia group cell lineage social media woodΒ part
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ScholarlyArticle:
headline:Flow Cytometric Monitoring of Residual Disease in Acute Leukemia
pageEnd:136
pageStart:123
image:https://media.springernature.com/w153/springer-static/cover/book/978-1-62703-357-2.jpg
genre:
Springer Protocols
isPartOf:
name:Hematological Malignancies
isbn:
978-1-62703-357-2
978-1-62703-356-5
type:Book
publisher:
name:Humana Press
logo:
url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
type:ImageObject
type:Organization
author:
name:Brent L. Wood
affiliation:
name:University of Washington
address:
name:Department of Laboratory Medicine, University of Washington, Seattle, USA
type:PostalAddress
type:Organization
type:Person
keywords:Immunophenotyping, Minimal residual disease, Flow cytometry, Acute lymphoblastic leukemia, Acute myeloid leukemia
description:Multiparameter flow cytometry offers the unique ability to simultaneously assess and correlate multiple cellular properties at the single cell level in a timely and efficient manner. Application of this technique to the detection of residual acute leukemia after therapy has been shown to be of singular importance to monitor response to therapy and provide prognostic information. Principles and methods that allow for the sensitive detection of acute leukemia following therapy are presented. The basic protocol outlines a simple and efficient method for the labeling of white cells with monoclonal antibodies directed against cell surface antigens. A second method describes a general method for the simultaneous assessment of surface and cytoplasmic antigens using a combination of fixation followed by membrane permeabilization. An illustrative panel of validated reagents currently in use for residual disease detection for acute lymphoblastic leukemia of B or T cell lineage as well as acute myeloid leukemia is provided. Principles of data analysis that allow for the reproducible detection of small populations of abnormal hematopoietic cells in peripheral blood and bone marrow are presented.
datePublished:2013
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affiliation:
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address:
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