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Title[redir]:
Fluorescence Measurement of Mitochondrial Membrane Potential Changes in Cultured Cells | SpringerLink
Description:
The mitochondrial membrane potential is the dominant component of the proton-motive force that is the potential term in the proton circuit linking electron transport to ATP synthesis and other energy-dependent mitochondrial processes. Cationic fluorescent probes have...
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Keywords {🔍}
mitochondrial, membrane, potential, nicholls, google, scholar, protocol, pubmed, cas, cultured, cells, chapter, article, privacy, cookies, content, information, publish, cell, research, search, bioenergetics, fluorescence, download, coimbra, springer, usd, personal, data, log, journal, measurement, david, book, methods, access, biol, ward, neurosci, author, ebook, optional, media, parties, policy, find, track, online, cite, molecular,
Topics {✒️}
month download article/chapter energy-dependent mitochondrial processes privacy choices/manage cookies device instant download editor information editors cultured cells protocol intact cell culture proton-motive force ward mw humana press mitochondrial membrane potential journal finder publish mitochondrial membrane potentials plasma membrane potential mitochondrial protonmotive force membrane-permeable cation european economic area detect gross qualitative rego ac inhibitor-mediated plasma cultured cerebellar neurons conditions privacy policy glutamate induced necrosis universidad de coimbra accepting optional cookies fluorescence measurement cationic fluorescent probes main content log online mitochondrial bioenergetics author correspondence protocol usd 49 protocol nicholls journal publish nicholls dg social media permissions reprints cultured cells protocol cite fluorescence signals privacy policy check access ethics access chapter log isolated mitochondria research moreno rights books a mitochondrial polarization cultured neurons
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ScholarlyArticle:
headline:Fluorescence Measurement of Mitochondrial Membrane Potential Changes in Cultured Cells
pageEnd:133
pageStart:119
image:https://media.springernature.com/w153/springer-static/cover/book/978-1-61779-382-0.jpg
genre:
Springer Protocols
isPartOf:
name:Mitochondrial Bioenergetics
isbn:
978-1-61779-382-0
978-1-61779-381-3
type:Book
publisher:
name:Humana Press
logo:
url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
type:ImageObject
type:Organization
author:
name:David G. Nicholls
affiliation:
name:Buck Institute for Research on Aging
address:
name:Buck Institute for Research on Aging, Novato, USA
type:PostalAddress
type:Organization
email:[email protected]
type:Person
keywords:Mitochondria, Membrane potential, Neuron, Glutamate, Proton-motive force, Proton electrochemical potential, Tetramethylrhodamine methyl ester, Rhodamine 123, JC-1
description:The mitochondrial membrane potential is the dominant component of the proton-motive force that is the potential term in the proton circuit linking electron transport to ATP synthesis and other energy-dependent mitochondrial processes. Cationic fluorescent probes have been used for many years to detect gross qualitative changes in mitochondrial membrane potentials in intact cell culture. In this chapter, I describe how these fluorescence signals may be used to obtain a semiquantitative measure of changes in mitochondrial membrane potential.
datePublished:2012
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affiliation:
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address:
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