
DOI . ORG {
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Title[redir]:
Morphological Identification of Melanopsin-Expressing Retinal Ganglion Cell Subtypes in Mice | SpringerLink
Description:
Melanopsin-expressing, intrinsically photosensitive retinal ganglion cells (ipRGCs) are a relatively recently discovered class of photoreceptor. ipRGCs can be subdivided into at least five subtypes (M1-M5), each of which has a distinct complement of morphological and...
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Keywords {🔍}
pubmed, article, retinal, google, scholar, ganglion, cas, central, cells, schmidt, intrinsically, photosensitive, mouse, protocol, melanopsinexpressing, subtypes, nature, neurosci, light, privacy, cookies, content, information, publish, morphological, cell, access, kofuji, springer, search, melanopsin, types, download, usd, function, personal, data, log, journal, research, phenotyping, mice, lee, tiffany, book, methods, iprgcs, properties, iprgc, chapter,
Topics {✒️}
melanopsin-expressing ganglion cells mouse ganglion-cell photoreceptors mouse ganglion-dell photoreceptors cell-type specific marker month download article/chapter mouse retinal phenotyping university hospital schleswig-holstein image-forming visual circuit retinal ganglion cells melanopsin-expressing neurons isolated retinal preparation privacy choices/manage cookies melanopsin detect light ecker jl pupillary light reflex dopamine amacrine cells device instant download altimus cm extrinsic light responses editor information editors central role patch clamp electrophysiology patch-clamp applications melanopsin-knockout mice rod/cone input sensitive rod pathway record light response protocol lee morphological identification ganglion cells image forming vision european economic area recently discovered class identified morphologically based modulate sleep independent melanopsin cells conditions privacy policy mouse development journal finder publish accepting optional cookies intrinsic physiological properties schmidt part protocol usd 49 main content log springer nature check access ethics access m4 cell protocol cite social media
Schema {🗺️}
ScholarlyArticle:
headline:Morphological Identification of Melanopsin-Expressing Retinal Ganglion Cell Subtypes in Mice
pageEnd:287
pageStart:275
image:https://media.springernature.com/w153/springer-static/cover/book/978-1-4939-7720-8.jpg
genre:
Springer Protocols
isPartOf:
name:Mouse Retinal Phenotyping
isbn:
978-1-4939-7720-8
978-1-4939-7719-2
type:Book
publisher:
name:Springer New York
logo:
url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
type:ImageObject
type:Organization
author:
name:Seul Ki Lee
affiliation:
name:Northwestern University
address:
name:Department of Neurobiology, Northwestern University, Evanston, USA
type:PostalAddress
type:Organization
type:Person
name:Tiffany M. Schmidt
affiliation:
name:Northwestern University
address:
name:Department of Neurobiology, Northwestern University, Evanston, USA
type:PostalAddress
type:Organization
email:[email protected]
type:Person
keywords:Melanopsin, Subtypes, Patch clamp electrophysiology, Dendritic stratification, SMI-32, Retina,
Opn4
, Mouse, Intrinsically photosensitive retinal ganglion cell
description:Melanopsin-expressing, intrinsically photosensitive retinal ganglion cells (ipRGCs) are a relatively recently discovered class of photoreceptor. ipRGCs can be subdivided into at least five subtypes (M1-M5), each of which has a distinct complement of morphological and physiological properties. ipRGC subtypes can be identified morphologically based on a combination of dendritic morphology and immunostaining for a cell-type specific marker. In this chapter, we describe methods for conclusively identifying each of the five ipRGC subtypes through a combination of patch clamp electrophysiology, Neurobiotin filling, visualization of ipRGC dendrites, and immunostaining for the marker SMI-32.
datePublished:2018
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isbn:
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name:Northwestern University
address:
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name:Northwestern University
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name:Department of Neurobiology, Northwestern University, Evanston, USA
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