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• Kraut A, Marcellin M, Adrait A, Kuhn L, Louwagie M, Kieffer-Jaquinod S et al (2009) Peptide storage: are you getting the best return on your investment?

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ScholarlyArticle:
      headline:Taking the Shortcut for High-Throughput Shotgun Proteomic Analysis of Bacteria
      pageEnd:285
      pageStart:275
      image:https://media.springernature.com/w153/springer-static/cover/book/978-1-4939-1261-2.jpg
      genre:
         Springer Protocols
      isPartOf:
         name:Host-Bacteria Interactions
         isbn:
            978-1-4939-1261-2
            978-1-4939-1260-5
         type:Book
      publisher:
         name:Springer New York
         logo:
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            type:ImageObject
         type:Organization
      author:
            name:Erica Marie Hartmann
            affiliation:
                  name:CEA, DSV, IBEB
                  address:
                     name:Lab Biochim System Perturb, CEA, DSV, IBEB, Bagnols-sur-Cèze, France
                     type:PostalAddress
                  type:Organization
            type:Person
            name:François Allain
            affiliation:
                  name:CEA, DSV, IBEB
                  address:
                     name:Lab Biochim System Perturb, CEA, DSV, IBEB, Bagnols-sur-Cèze, France
                     type:PostalAddress
                  type:Organization
                  name:Bertin Technologies, Parc d’activités du Pas du Lac
                  address:
                     name:Bertin Technologies, Parc d’activités du Pas du Lac, Montigny-le-Bretonneux, France
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Jean-Charles Gaillard
            affiliation:
                  name:CEA, DSV, IBEB
                  address:
                     name:Lab Biochim System Perturb, CEA, DSV, IBEB, Bagnols-sur-Cèze, France
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Olivier Pible
            affiliation:
                  name:CEA, DSV, IBEB
                  address:
                     name:Lab Biochim System Perturb, CEA, DSV, IBEB, Bagnols-sur-Cèze, France
                     type:PostalAddress
                  type:Organization
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            name:Jean Armengaud
            affiliation:
                  name:CEA, DSV, IBEB
                  address:
                     name:Lab Biochim System Perturb, CEA, DSV, IBEB, Bagnols-sur-Cèze, France
                     type:PostalAddress
                  type:Organization
            email:[email protected]
            type:Person
      keywords:High-throughput proteomics, Quantitative proteomics, Bacterial proteome, Spectral count, Tandem mass spectrometry
      description:Currently, proteomic tools are able to establish a complete list of the most abundant proteins present in a sample, providing the opportunity to study at high resolution the physiology of any bacteria for which the genome sequence is available. For a comprehensive list, proteins should be first resolved into fractions that are then proteolyzed by trypsin. The resulting peptide mixtures are analyzed by a high-throughput tandem mass spectrometer that records thousands of MS/MS spectra for each fraction. These spectra are then assigned to peptides, which are used as evidence of the existence of proteins. In addition to generating a list of protein identifications, this shortcut to proteomics uses the number of spectra recorded for each protein to quantify the observations. Here, we describe one of the most simple sample preparation methods for high-throughput proteomics of bacteria, as well as the subsequent data processing to extract quantitative information based on the spectral count approach.
      datePublished:2014
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Book:
      name:Host-Bacteria Interactions
      isbn:
         978-1-4939-1261-2
         978-1-4939-1260-5
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      name:Springer New York
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      address:
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         type:PostalAddress
      name:CEA, DSV, IBEB
      address:
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         type:PostalAddress
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      address:
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      name:Erica Marie Hartmann
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               type:PostalAddress
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      name:François Allain
      affiliation:
            name:CEA, DSV, IBEB
            address:
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               type:PostalAddress
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               type:PostalAddress
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      affiliation:
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            address:
               name:Lab Biochim System Perturb, CEA, DSV, IBEB, Bagnols-sur-Cèze, France
               type:PostalAddress
            type:Organization
      name:Olivier Pible
      affiliation:
            name:CEA, DSV, IBEB
            address:
               name:Lab Biochim System Perturb, CEA, DSV, IBEB, Bagnols-sur-Cèze, France
               type:PostalAddress
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      name:Jean Armengaud
      affiliation:
            name:CEA, DSV, IBEB
            address:
               name:Lab Biochim System Perturb, CEA, DSV, IBEB, Bagnols-sur-Cèze, France
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      name:Lab Biochim System Perturb, CEA, DSV, IBEB, Bagnols-sur-Cèze, France
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