
DOI . ORG {
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Title[redir]:
Detection of SLE Antigens in Neutrophil Extracellular Traps (NETs) | SpringerLink
Description:
Neutrophils are sentinel cells of the innate immune system with a primary role of clearing extracellular pathogens. The release of weblike structures decorated with granular proteins called neutrophil extracellular traps (NETs) has recently been implicated in the...
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Keywords {π}
pubmed, article, google, scholar, lupus, systemic, erythematosus, cas, neutrophil, extracellular, protocol, traps, kaplan, rheumatol, nets, central, privacy, cookies, content, information, publish, carmonarivera, neutrophils, immunol, autoantibodies, research, search, sle, antigens, access, antibodies, patients, download, springer, usd, personal, data, log, journal, book, molecular, proteins, autoimmune, diseases, chapter, zychlinsky, zhao, wang, histone, biol,
Topics {βοΈ}
month download article/chapter protocol carmona-rivera intramural research program systemic lupus erythematosus privacy choices/manage cookies hydralazine-induced lupus device instant download van venrooij wj van der vlag neutrophil extracellular traps neutrophil extracellular trap editor information editors mediate organ damage clearing extracellular pathogens neutrophil cytoplasm components antineutrophil cytoplasmic antibodies anti-dsdna antibodies anti-lactoferrin antibodies european economic area innate immune system weblike structures decorated peptidylarginine deiminase inhibition dna-peptide complexes clinico-serological correlations anti-alpha-enolase journal finder publish conditions privacy policy expose immunostimulatory molecules exeter medical school circulating anti-elastase accepting optional cookies carmona-rivera cathepsin g-anca smolen js main content log protocol usdΒ 49 describe protocols antineutrophil cytoplasmic autoantibodies detect sle antigens berthier cc protocol cite check access ethics access social media permissions reprints histone h2b humana press immunostimulatory proteins neutrophil elastase journal publish
Schema {πΊοΈ}
ScholarlyArticle:
headline:Detection of SLE Antigens in Neutrophil Extracellular Traps (NETs)
pageEnd:161
pageStart:151
image:https://media.springernature.com/w153/springer-static/cover/book/978-1-4939-0326-9.jpg
genre:
Springer Protocols
isPartOf:
name:Systemic Lupus Erythematosus
isbn:
978-1-4939-0326-9
978-1-4939-0325-2
type:Book
publisher:
name:Springer New York
logo:
url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
type:ImageObject
type:Organization
author:
name:Carmelo Carmona-Rivera
affiliation:
name:National Institute of Arthritis and Musculoskeletal and Skin Diseases, National Institutes of Health
address:
name:Systemic Autoimmunity Branch, Intramural Research Program, National Institute of Arthritis and Musculoskeletal and Skin Diseases, National Institutes of Health, Bethesda, USA
type:PostalAddress
type:Organization
type:Person
name:Mariana J. Kaplan
affiliation:
name:National Institute of Arthritis and Musculoskeletal and Skin Diseases, National Institutes of Health
address:
name:Systemic Autoimmunity Branch, Intramural Research Program, National Institute of Arthritis and Musculoskeletal and Skin Diseases, National Institutes of Health, Bethesda, USA
type:PostalAddress
type:Organization
type:Person
keywords:Leukocytes, Nuclear components, Granular proteins, Reactive oxygen species
description:Neutrophils are sentinel cells of the innate immune system with a primary role of clearing extracellular pathogens. The release of weblike structures decorated with granular proteins called neutrophil extracellular traps (NETs) has recently been implicated in the pathogenesis of inflammatory and autoimmune diseases. Indeed, NETs may represent an important source of autoantigens and immunostimulatory proteins in systemic lupus erythematosus (SLE). In this chapter, we describe protocols to isolate human peripheral neutrophils, to generate and isolate NETs, and to detect SLE antigens in NETs using immunofluorescence and immunoblot.
datePublished:2014
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Book:
name:Systemic Lupus Erythematosus
isbn:
978-1-4939-0326-9
978-1-4939-0325-2
Organization:
name:Springer New York
logo:
url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
type:ImageObject
name:National Institute of Arthritis and Musculoskeletal and Skin Diseases, National Institutes of Health
address:
name:Systemic Autoimmunity Branch, Intramural Research Program, National Institute of Arthritis and Musculoskeletal and Skin Diseases, National Institutes of Health, Bethesda, USA
type:PostalAddress
name:National Institute of Arthritis and Musculoskeletal and Skin Diseases, National Institutes of Health
address:
name:Systemic Autoimmunity Branch, Intramural Research Program, National Institute of Arthritis and Musculoskeletal and Skin Diseases, National Institutes of Health, Bethesda, USA
type:PostalAddress
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name:Carmelo Carmona-Rivera
affiliation:
name:National Institute of Arthritis and Musculoskeletal and Skin Diseases, National Institutes of Health
address:
name:Systemic Autoimmunity Branch, Intramural Research Program, National Institute of Arthritis and Musculoskeletal and Skin Diseases, National Institutes of Health, Bethesda, USA
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type:PostalAddress
type:Organization
PostalAddress:
name:Systemic Autoimmunity Branch, Intramural Research Program, National Institute of Arthritis and Musculoskeletal and Skin Diseases, National Institutes of Health, Bethesda, USA
name:Systemic Autoimmunity Branch, Intramural Research Program, National Institute of Arthritis and Musculoskeletal and Skin Diseases, National Institutes of Health, Bethesda, USA
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