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Hepatology
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α. Liver and peripheral blood mononuclear cell (PBMC) samples were used for total genome microarray analysis. Most induced genes achieved maximal response within 4 hours, began to decline by 8 hours, and were at baseline levels by 24 hours post-inoculation, a time when high levels of circulating pegylated IFN-α were still present. The rapid downregulation of the IFN-α response may be involved in the transition between the observed phase I and phase II viral kinetics during IFN-α therapy in HCV-infected patients. The response to all three forms of IFN-α was similar; thus, the reasons for previous failures in antiviral treatment of chimpanzees with human IFN-α were not due to species specificity of IFN-α. The response to IFN-α was partially tissue-specific. A total of 1,778 genes were altered in expression by twofold or more by IFN-α, with 538 and 950 being unique to the liver or PBMC, respectively. Analysis of the IFN-α and IFN-γ responses in primary chimpanzee and human hepatocytes were compared as well. IFN-α and IFN-γ induced partially overlapping sets of genes in hepatocytes.In conclusion, the response to IFN-α is largely tissue-specific, and the response is rapidly downregulatedin vivo,which may have a significant influence on the kinetics of antiviral response.Supplementary material for this article can be found on the HEPATOLOGY website (http://interscience.wiley.com/jpages/0270-9139/suppmat/index.html).(Hepatology 2006;43:961–972.)...
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Keywords {🔍}
ifnα, response, liver, aasld, hepatology, log, journal, access, subscribe, issue, alerts, abstract, hepatitis, chimpanzees, kinetics, antiviral, study, human, genes, hours, register, member, logo, browse, submit, article, previous, viral, rapid, downregulation, buy, therapy, chimpanzee, pbmc, total, analysis, induced, levels, phase, partially, tissuespecific, ifnγ, website, copyright, american, association, diseases, text, subscribers, members,
Topics {✒️}
aasld members log circulating pegylated ifn-α human-pegylated ifn-α open access policy partially tissue-specific ifn-γ responses individual subscribers log induced antiviral response institutional users access largely tissue-specific rapidly downregulatedin vivo /jpages/0270-9139/suppmat/index artificial intelligence training liver diseases full text access hcv-infected patients human ifn-α 24 hours post-inoculation ifn-α response hepatology website ifn-α therapy journal tables register subscribe service request 800-638-3030 21167 buy abstract hepatology 2006 ifn-α liver observed phase journal antiviral response website subscribe previous failures interferon-α transcriptional response kinetics lanford antiviral treatment rapid downregulation interferon-alpha completely understood single doses baseline levels high levels species specificity human hepatocytes significant influence supplementary material email inbox rights reserved data mining
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