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Comparing DNA integration site clusters with scan statistics | Bioinformatics | Oxford Academic
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Abstract. Motivation: Gene therapy with retroviral vectors can induce adverse effects when those vectors integrate in sensitive genomic regions. Retroviral
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Keywords {π}
sites, windows, regions, number, vector, clumps, integration, region, window, google, vectors, odds, scholar, false, iss, discoveries, mse, expected, worldcat, genomic, set, critical, jurkat, data, avr, vol, power, discovered, versus, site, gene, clump, cutpoints, methods, therapy, target, crossrefpubmed, bases, width, supplementary, comparison, background, discovery, dna, hiv, genome, infection, chromosome, figure, values,
Topics {βοΈ}
computational biology oxford academic bioinformatics mathematics books journals oxford university press hacein-bey-abina signature search ccrr google scholar latest university press partners article sign genome-wide insertional mutagenesis scid-x1 gene therapy volume 30 june 2014 term dose-response transcriptomic analysis maryland view design wet-bench follow supplementary data show search genome-wide background odds enhancing variant interpretation genome-wide variation references discussion yield patient-specific clumps single fixed-width scan recursively splits data introduction comprehensive genomic access marketing citing articles clinical gene therapy target false discovery genomic data analysis gene-corrected subjects hiv primary isolate discovered windows results bioinformatics online access accessibility contact false discovery rate human gene therapy successful gene therapy basic data form cited dose-l1000 cd4+ versus jurkat supplementary data author 2014 data collection methods vector reflect preferences avr versus mse challenges clumping methods
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ScholarlyArticle:
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name:Comparing DNA integration site clusters with scan statistics
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url:https://dx.doi.org/10.1093/bioinformatics/btu035
inLanguage:en
copyrightHolder:Oxford University Press
copyrightYear:2025
publisher:Oxford University Press
author:
name:Berry, Charles C.
affiliation:Division of Biostatistics and BioInformatics, Department of Family and Preventive Medicine, University of California at San Diego, La Jolla, CA 92093-0901 and Department of Microbiology, Perelman School of Medicine at the University of Pennsylvania, 425 Johnson Pavilion, Philadelphia, PA 19104-6076, USA
type:Person
name:Ocwieja, Karen E.
affiliation:Division of Biostatistics and BioInformatics, Department of Family and Preventive Medicine, University of California at San Diego, La Jolla, CA 92093-0901 and Department of Microbiology, Perelman School of Medicine at the University of Pennsylvania, 425 Johnson Pavilion, Philadelphia, PA 19104-6076, USA
type:Person
name:Malani, Nirav
affiliation:Division of Biostatistics and BioInformatics, Department of Family and Preventive Medicine, University of California at San Diego, La Jolla, CA 92093-0901 and Department of Microbiology, Perelman School of Medicine at the University of Pennsylvania, 425 Johnson Pavilion, Philadelphia, PA 19104-6076, USA
type:Person
name:Bushman, Frederic D.
affiliation:Division of Biostatistics and BioInformatics, Department of Family and Preventive Medicine, University of California at San Diego, La Jolla, CA 92093-0901 and Department of Microbiology, Perelman School of Medicine at the University of Pennsylvania, 425 Johnson Pavilion, Philadelphia, PA 19104-6076, USA
type:Person
description:Abstract. Motivation: Gene therapy with retroviral vectors can induce adverse effects when those vectors integrate in sensitive genomic regions. Retroviral
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headline:Comparing DNA integration site clusters with scan statistics
image:https://oup.silverchair-cdn.com/oup/backfile/Content_public/Journal/bioinformatics/30/11/10.1093_bioinformatics_btu035/2/m_bioinformatics_30_11_1493_f2.gif?Expires=1813974148&Signature=wDcYfdR0nSihEA2IX0YfhZL~2QHYyVi2rfecY3An8vC6WmbRywVdmC2UITTVWHFAuNy~S6bMn4Wo72uYG4uKnTTj-17hlCeraBGthGbwzbjbd7TzKE20L~cbFqkYXPvET0Voe5ShE5PFTEnc5puOaqgxW-w0ZpnWPeIzjQ389T7krzKrxDDr2UaEitia-wSxyACy865NFjrjnmjRF~AycJb2B2QSdVOC9GslqL1WgwvGE0xA0IJ4WkYfFv125~Y59jokw7MeBdY0M-SIbGegdTxvD9uJaeybmZ3ImgBehpRD~Hp2rbv1iG0HYZUszucaDMXVMoWFc1QmHlb1B~mUgQ__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA
image:alt:Clustering of ISs by recovery method. A region of chromosome 17 is shown and marked by locations of HIV vector ISs recovered by each of two methods (see Section 3.3 for details). Sites recovered after cleavage of genomic DNA with Mse are marked with circles; those recovered after cleavage with the Avr cocktail are shown by the triangles. The circles and triangles are shown evenly spaced along the x-axis (lower middle) for ease of visualization, then connected by lines to the chromosome scaffold (bottom) to show their distribution on the chromosome. A clump, or genomic interval enriched for a recovery method, is discovered as follows: Windows containing between 15 and 75 sites were tested for enrichment of Avr or Mse, but only those passing the enrichment test are shown. Each horizontal bar (upper section of figure) represents a window that spans the x-values for a group of sites that show enrichment for Mse. (In this region, no such group shows enrichment for Avr.) The bars are grouped together according to the number of sites spanned and the number of Mse sites required to declare enrichment (see text for details of how the cutpoints were chosen). Those numbers are given just above the right edge of each group as a fraction (required/spanned). The depth, i.e. number of different window size groups over a site, is indicated at the top. Sites with greatest depth are always included in the final clump, but sites at the edges are screened using a likelihood criterion; in this case several sites on each side are excluded. Dashed vertical lines enclose the sites that were ultimately assigned to the clump
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datePublished:2014-06-01
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Person:
name:Berry, Charles C.
affiliation:Division of Biostatistics and BioInformatics, Department of Family and Preventive Medicine, University of California at San Diego, La Jolla, CA 92093-0901 and Department of Microbiology, Perelman School of Medicine at the University of Pennsylvania, 425 Johnson Pavilion, Philadelphia, PA 19104-6076, USA
name:Ocwieja, Karen E.
affiliation:Division of Biostatistics and BioInformatics, Department of Family and Preventive Medicine, University of California at San Diego, La Jolla, CA 92093-0901 and Department of Microbiology, Perelman School of Medicine at the University of Pennsylvania, 425 Johnson Pavilion, Philadelphia, PA 19104-6076, USA
name:Malani, Nirav
affiliation:Division of Biostatistics and BioInformatics, Department of Family and Preventive Medicine, University of California at San Diego, La Jolla, CA 92093-0901 and Department of Microbiology, Perelman School of Medicine at the University of Pennsylvania, 425 Johnson Pavilion, Philadelphia, PA 19104-6076, USA
name:Bushman, Frederic D.
affiliation:Division of Biostatistics and BioInformatics, Department of Family and Preventive Medicine, University of California at San Diego, La Jolla, CA 92093-0901 and Department of Microbiology, Perelman School of Medicine at the University of Pennsylvania, 425 Johnson Pavilion, Philadelphia, PA 19104-6076, USA
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