
NCBI . NLM . NIH . GOV {
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Title[redir]:
Molecular basis for the distinct cellular functions of the Lsm1–7 and Lsm2–8 complexes - PMC
Description:
Eukaryotes possess eight highly conserved Lsm (like Sm) proteins that assemble into circular, heteroheptameric complexes, bind RNA, and direct a diverse range of biological processes. Among the many essential functions of Lsm proteins, the ...
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Keywords {🔍}
lsm, rna, binding, google, scholar, doi, pubmed, pmc, article, complex, free, phosphate, fig, cyclic, structure, rnas, bound, proteins, carboxyterminal, buffer, pombe, end, structures, complexes, binds, data, ring, mrna, protein, supplemental, molecular, tightly, cerevisiae, snrna, bind, terminal, pat, affinity, final, single, sodium, cell, biol, determined, adenosine, lsmδc, nacl, montemayor, region, oligou,
Topics {✒️}
point isopropyl β-d-1-thiogalactopyranoside /n-rich aggregation-prone regions residual fo–fc density remaining fo–fc density carboxy-terminal alpha-helical extension [google scholar] veretnik undergo adenine/uridine-rich element existing cryo-em structures pmc beta search comprehensive python-based system carboxy-terminally truncated lsm1–7 nuclear pre-mrna decapping tris-tricine sds-page carboxy-terminal histidine residue unique carboxy-terminal extension amino-terminal alpha helix multi-orf expression system footnotes article including pre-mrna splicing high-throughput crystallization screening glutamine/asparagine-rich domain lsm1 carboxy-terminal region lsm1 carboxy-terminal helix elongated carboxy-terminal region lsm1 carboxy-terminal domain org/cgi/doi/10 phase-separated processing bodies determining high-resolution structures lsm8 carboxy-terminal histidine full-length u6 rna marti-renom electron density map lsm2–8/rna complexes mixed decapping enzyme dcp2 vitro binding assays sm/lsm genes provide 1 mg/ml bsa u6 snrna-lsm2–8 complex nitrogen starvation-induced autophagy de-ac02-06ch11357 lsm1 carboxy-terminal deletion shows electron density lsm2–8 specifically recognizes weak electron density lsm1-7/pat1 complex binds carboxy-terminal region carboxy-terminal histidine carboxy-terminal helix anion exchange chromatography anion-exchange chromatography
Questions {❓}
- AU-rich elements and associated factors: are there unifying principles?
- How do site-specific DNA-binding proteins find their targets?
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