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Keywords {🔍}

cells, tentc, doi, rna, fig, cell, mice, supplementary, google, scholar, analysis, pubmed, polya, mrna, pmc, data, isolated, clone, article, buffer, expression, min, differentiation, free, activated, spleen, sequencing, response, igg, protein, days, tlr, performed, tentcgfp, mrnas, polyadenylation, igs, serum, levels, antibodies, mature, flow, cytoplasmic, proteins, pcs, transcripts, analyzed, staining, cat, direct,

Topics {✒️}

mtent5c_egfp-rf_1f/mtent5c_rf-topo_1r primer pairs mtent5c-gfp_short_1f/ mtent5c-gfp-short_1r primers pl/en/services/mass-spectrometry-lab coli bl21-codonplus-ril strain author contributions anti-cd21/cd35 percp cy5 tent5c-tev-egfp donor flanked high-affinity anti-gfp nanobodies tent5c c-terminal-gfp knock anti-cd45r/b220 percp cy5 turbo dna-free kit competing interests 1 mg/ml mtsea-biotin-xx pkk-tev-egfp plasmid pseudo-color dot blots data availability statements full-length mrna sequences high-resolution mass spectrometry mtent5c_topo-lf_1f/mtent5c_lf-tev_1r sas-mx sp-10 kit b6-tent5c-gfp/gfp/tar ptopo/f46c/tev-egfp type 1 t-cell-independent antigen high-throughput sequencing reads anti-cd24 percp cy5 code availability zinc-finger protein zfp318 gold rrna-removal kit pmc beta search license information pmcid 10  µg/ml lps-ek standard anti-cd45r/b220 apc tev-egfp coding sequence 60 bp homology arms 75-nt paired-end mode hrp-conjugated secondary antibodies classical rna-seq experiments32 rna 3ʹ-terminome research fc block anti-cd16/32 optimizes b-cell function accumulate membrane-bound igg1 anti-tnp igg concentrations high-resolution ms analyses verify rna-seq data 3ʹ-end cleavage machinery 1  µg/ml fla-st rna-binding protein hnrnpll 12–15% sds–page gels standard luria-broth ig-secreting pcs requires

Questions {❓}

• RNA turnover in human mitochondria: more questions than answers?
• Stressed-out B cells?

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