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Title[redir]:
Aifm2, a NADH oxidase, supports robust glycolysis and is required for cold- and diet-induced thermogenesis - PMC
Description:
Brown adipose tissue (BAT) is highly metabolically active tissue to dissipate energy via UCP1 as heat, and BAT mass is correlated negatively with obesity. The presence of BAT/BAT-like tissue in humans renders BAT as attractive target against obesity ...
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Keywords {🔍}
aifm, bat, cells, mice, fig, thermogenesis, glycolysis, ocr, doi, mitochondrial, pubmed, google, scholar, nadh, glucose, control, activity, mitochondria, tissue, brown, cold, increased, fold, lower, increase, higher, oxidase, nadnadh, pmc, levels, fraction, left, adipose, cell, nad, showed, article, wat, iwat, iso, ucp, data, cytosolic, nde, free, temperature, results, uncoupled, oxidation, tissues,
Topics {✒️}
serine/threonine kinase b-raf n-terminal a-amino group inserted loxp-stop-loxp casette saccharomyces cerevisiae nde1 cold exposure/β-adrenergic stimulation p53-inducible apoptogenic gene p53-inducible gene downregulated loxp-stop-loxp cassette van marken lichtenbelt pmc beta search intracellular l-lactate levels putative nadh-binding site affects cellular nad/nadh crispr-cas9 nickase system prdm16/ctbp transcriptional complex measure nad/nadh levels intracellular l-lactate concentration lysed 48h post-transfection c-terminal deletion constructs differentiated 3t3-l1 adipocytes glycerol-3-phosphate shuttle human n-myristoylated proteins endogenous β-receptor nadh/nad oxidoreductase domain n-terminal domain fatty acid synthesis ucp1-cre aav7 virus fuel cold/diet-induced thermogenesis nadh/nad redox state increasing nad levels aifm2-tg mice showed decreased cytosolic nadh/nad increased nad/nadh ratio aifm2-tg mice survived cytoplasmic nadh/nad state ameliorate diet-induced obesity performed site-directed mutagenesis isolating fat droplets biochemical assay based gfp-tagged aifm2 lentivirus imaging cytosolic nadh-nad lower nad/nadh level diet-induced thermogenesis hai abolishes diet-induced thermogenesis uc berkeley acuc β-adrenergic stimulation cold-induced thermogenesis depends malate-aspartate shuttle fatty acid anabolic increase nad/nadh ratio
Questions {❓}
- Adaptive thermogenesis in white adipose tissue: is lactate the new brown(ing)?
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