
NCBI . NLM . NIH . GOV {
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Title[redir]:
Accurate detection of m6A RNA modifications in native RNA sequences - PMC
Description:
The epitranscriptomics field has undergone an enormous expansion in the last few years; however, a major limitation is the lack of generic methods to map RNA modifications transcriptome-wide. Here, we show that using direct RNA sequencing, ...
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Keywords {π}
rna, doi, modifications, mamodified, data, modification, features, sites, pubmed, fig, google, scholar, pmc, sequencing, yeast, supplementary, article, unmodified, ime, free, frequency, reads, current, direct, accuracy, sets, modified, basecalled, mismatch, deletion, kmers, rrach, site, sequences, strains, cell, identify, quality, nature, information, methods, biological, errors, observed, raw, vitro, shown, svm, basecalling, algorithm,
Topics {βοΈ}
author contributions single-molecule real-time detection m6a-modified rrach k-mers competing interests base-called m6a-modified reads base-called βerrorsβ information dynabeads mrna-direct kit m6a-modified k-mers included data availability statements data availability statement real-time selective sequencing single-molecule sequencing detection n6-methyladenosine rna demethylase pmc beta search decreased base-calling qualities high-resolution mapping reveals unmodified rrach k-mers accurately identify n6-methyladenosine m6a-modified data sets signal-based machine learning m6a-modified rrach sites single-nucleotide-resolution mapping wild-type yeast strains settings -ax map-ont m6a-unmodified data sets base-called reads sequences reproducible base-calling βerrorsβ data availability m6a-modified yeast rnas vivo base-called features wild-type yeast reads extract base-called features code availability rnaclean xp beads tombo correctly predicts license information pmcid m6a-motif rrach set base-calling algorithms transcriptome-wide fashion transcriptome-wide distribution yeast ime4-knockout strains rrach k-mers chemical-based detection previous studies probing direct rna sequencing m6a writers reveals 31501426 abstract introduction discussion acknowledgements
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