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Title[redir]:
Suppression of Oct4 by Germ Cell Nuclear Factor Restricts Pluripotency and Promotes Neural Stem Cell Development in the Early Neural Lineage - PMC
Description:
The earliest murine neural stem cells are leukemia inhibitory factor (LIF)-dependent, primitive neural stem cells, which can be isolated from embryonic stem cells or early embryos. These primitive neural stem cells have the ability to differentiate ...
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Keywords {🔍}
gcnf, cells, oct, neurospheres, neural, stem, cell, doi, embryos, dnscs, fig, dnscderived, pnscderived, pnscs, expression, increased, supplemental, transition, primary, compared, lif, google, scholar, pubmed, secondary, numbers, spheres, early, derived, fgf, differentiation, pnsc, pluripotency, mouse, clonal, primitive, promoter, colonies, development, embryonic, definitive, dissociated, dnsc, significantly, lineage, wwwjneurosciorg, material, pmc, factor, control,
Topics {✒️}
wild-type dnsc-derived colonies anti-β-actin monoclonal antibody real-time quantitative rt-pcr lif-dependent pnsc-derived neurospheres anti-human α-1-fetoprotein serum-free floating culture pou-family transcription factor anti-gfp monoclonal antibody anti-pax6 monoclonal antibody pmc beta search anti-oct4 monoclonal antibody orphan nuclear receptor dnsc-derived neurospheres generated dnsc-derived neurospheres derived pnsc-derived neurospheres generated full-length gcnf cdna primary dnsc-derived neurospheres primary pnsc-derived neurospheres dnsc-derived neurosphere colonies anti-gfp polyclonal antibody clonal dnsc-derived neurospheres dnsc-derived clonal neurospheres oct4 full-length cdna pnsc-derived neurospheres colonies secondary dnsc-derived neurospheres clonal pnsc-derived neurospheres tertiary dnsc-derived neurospheres anti-nestin polyclonal antibody pmc copyright notice [google scholar] nichols [google scholar] shimozaki tissue-tek mounting media dnsc-derived neurospheres compared gcnf+/+ dnsc-derived neurospheres gcnf−/− dnsc-derived neurospheres gcnf−/− pnsc-derived neurospheres gcnf+/+ pnsc-derived neurospheres primary pnsc-derived spheres wt dnsc-derived neurospheres normal rabbit igg qualitative rt-pcr analysis analyzed dnsc-derived neurospheres serum-free media lentiviral vector-mediated transduction quantitative rt-pcr analysis gfp-positive donor cells quantitative rt-pcr revealed de novo methylation donor lif-dependent pnscs serum-free medium
Questions {❓}
- Sustained Oct4 is correlated with the GCNF−/− phenotypes in pNSCs and dNSCs, but is the sustained Oct4 functionally responsible for these phenotypes?
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