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Title[redir]:
Non-invasive measurement of mRNA decay reveals translation initiation as the major determinant of mRNA stability - PMC
Description:
The cytoplasmic abundance of mRNAs is strictly controlled through a balance of production and degradation. Whereas the control of mRNA synthesis through transcription has been well characterized, less is known about the regulation of mRNA turnover, ...
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Keywords {๐}
mrna, figure, decay, mrnas, doi, translation, data, stability, cells, initiation, supplement, rna, halflife, pubmed, halflives, model, elongation, transcripts, google, scholar, experiments, polya, kwy, transcript, measurements, pmc, article, results, labeling, act, cell, free, treated, rates, yeast, total, min, ribosomes, replicates, analysis, hippuristanol, elife, effects, cis, experiment, degradation, codon, transcriptome, parker, response,
Topics {โ๏ธ}
pgpd1-lexa-b112 cdc33-iaa7-3v5 prs425 p4xlexocyc1-3xgst-v5-24xpp7sl-tcyc1-natnt2-hr2_chr2 p4xlexocyc1-3xgst-v5-24xpp7sl-tcyc1-natnt2 cdc33-w104f-e105a henceforth cdc33โcap prs425-p4lexocyc1-cdc33โcapโg-3v5 pnh604-pgpd1-lexa-ebd-b112 pnh603-pgpd1-lexa-ebd-b112 pgpd1-lexa-b112 cells harboring pgpd1-ostir1 cdc33-iaa7-3v5 prs425-p4lexocyc1-cdc33โg-3v5 pgdp1-lexa-b112 cells harboring pgpd1-lexa-ebd-b112 prs425-p4lexocyc1-cdc33โcap-3v5 prs425-p4lexocyc1-cdc33-3v5 plasmids prs425-p4xlexocyc1-cdc33 ๐ ๐ ๏ฟฝ prs425-p4lexocyc1-cdc33โcap prs425-p4lexocyc1-cdc33โcapโ lexa-ebd-b112 pfa6a-iaa7-3v5-kanmx6 dominant-negative eif4e/degron depletion 4tu-pulse/uracil-chase labeling scheme induces dhh1-dependent p-bodies cdc33-iaa7-3v5 cdc33-3v5-iaa7 14c-adenine pulse-labeling experiments prs425-p4lexocyc1-cdc33 prs425-p4lexocyc1-cdc33โ pmc beta search ๐ ๐ 14c-adenosine incorporation agrees dcp2-mcherry expressing cells existing 4tu-pulse/uracil-chase inverted epi-fluorescence microscope funding information media lacking ฮฒ-estradiol ฮฒ-estradiol inducible promoter pdhh1-dhh1q-motif-gfp ๐ โ translation factor-protection model translation-factor protection model pdhh1-dhh13x-rna-gfp pnh605-pgpd1-ostir1 pre-salt shocked cells stalled ribosome-triggered decay stem-loop reporter mrna editorial decision letter translation factor-protection models v5 tagged proteins plating wild-type cells
Questions {โ}
- 2ฮผg/ml CHX?
- Is it possible that on some mRNAs, the presence of suboptimal codons is equally important, with ribosomes containing empty A sites being recognized by Dhh1 in the manner suggested by Coller and Green?
- Is there a way to eliminate this alternative?
- Subsection โAn improved non-invasive metabolic labeling protocol reveals that the yeast transcriptome is highly unstableโ: Perhaps step 7 is intended?
- The manuscript addresses an important and timely issue in the field: Is decay rate coupled to stalled ribosomes, or to poor translation initiation?
- Was Figure 2โfigure supplement 1 cited in Results section?
- Was Figure 2โfigure supplement 1 cited in Results section?
- Were these cells also treated with hippuristanol?
- What are the consequences of these perturbations to translation and their effect on mRNA decay at the cellular level?
- Why did the authors use polyA pulldown in their protocol?
- Nutrient-rich medium with glucose as carbon source?
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