
NCBI . NLM . NIH . GOV {
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Title[redir]:
PSA-selective activation of cytotoxic human serine proteases within the tumor microenvironment as a therapeutic strategy to target prostate cancer - PMC
Description:
Prostate cancer is the most diagnosed malignancy and the second leading cause of cancer-related death in American men. While localized therapy is highly curative, treatments for metastatic prostate cancer are largely palliative. Thus, new innovative ...
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Keywords {🔍}
psa, cells, gzmb, ekpsagzmb, ekpsatrp, cell, cancer, prostate, protein, activity, substrate, doi, human, figure, activated, effects, serum, trp, enzymatic, activation, protease, ecm, proteins, proteases, free, treated, lncap, google, scholar, media, pubmed, active, psaactivated, tumor, incubated, growth, psagzmb, pmc, plated, extracellular, trypsin, due, enzyme, microenvironment, shown, zymogen, peptide, kgissqy, psatrp, hour,
Topics {✒️}
slide-a-lyzer dialysis cassette anti-rabbit hrp-linked igg isolated psa-digested ek-psa-gzmb opti-mem low-serum media recombinant ek-psa-gzmb/trp proteins ek-psa-gzmb showing substantial michaelis-menten enzyme kinetics activated ek-psa-gzmb showed ek-psa-trp showed activation inhibits ek-psa-trp robustly activated ek-psa-trp reducing conditions ek-psa-gzmb di-sulfide linked dimer psa treated ek-psa-gzmb ek-psa-trp pro-drugs anti-ddddk polyclonal antibody n-terminal isoleucine residue lncap-bearing nude mice pore-forming protein perforin proliferation-independent cytotoxic payload c-terminal reactive cysteine pmc beta search yielding n-termini beginning activated cytotoxic t-lymphocytes c-terminal histidine tag michaelis-menten kinetics n-terminal ek piece cancer-specific protease psa psa-activated protoxin approach psa-activated variants suggesting phase i-iii testing inactive proliferation-independent cytotoxin anti-tumor immune response n-terminal ek sequence activated ek-psa-trp gzmb deemed psa-trp n-terminal amino acids ek-psa-trp drug ek-psa-gzmb robustly synergistic anti-tumor effect 500 nm ek-psa-trp protease-induced ecm damage michaelis-menten experiments ek-psa-gzmb retained characterized pro-forms activated activated ek-psa-gzmb gpr-pna release assay psa-activated pro-drug activate pro-apoptotic protein n-terminal pro-peptides
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