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Title[redir]:
Non-canonical activation of DAPK2 by AMPK constitutes a new pathway linking metabolic stress to autophagy - PMC
Description:
Autophagy is an intracellular degradation process essential for adaptation to metabolic stress. DAPK2 is a calmodulin-regulated protein kinase, which has been implicated in autophagy regulation, though the mechanism is unclear. Here, we show that ...
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Keywords {🔍}
dapk, ser, doi, ampk, phosphorylation, cells, kinase, autophagy, beclin, pubmed, google, scholar, protein, cam, fig, cell, binding, activation, pmc, catalytic, article, assay, transfected, activity, performed, domain, free, mutant, incubated, flagdapk, buffer, hekt, sirna, phosphorylated, control, shown, treated, biol, site, mutants, mlc, response, experiments, autophosphorylation, proteins, independent, thr, test, sdspage, supplementary,
Topics {✒️}
hrp-conjugated goat anti-mouse beta-catenin-induced anoikis resistance regulated ser/thr kinase8 edta-free protease-inhibitor tablet pmc beta search anti-rabbit secondary antibodies s289d phospho-mimicking mutant ion-trap mass spectrometer cgmp-dependent protein kinase puncta area/cell area auto-regulatory domain binds sqstm1-dependent selective autophagy calmodulin-regulated protein kinase beclin-1/atg14/vps34 complex activity protein-fragment complementation assays dfcp1-gfp puncta specifically cam auto-regulatory domain39 cam auto-regulatory domains9 phospho-silencing mutant s289a hek293 gfp-lc3b cells double-membrane autophagic vesicles18 methods cell culture discovering protein–protein interactions protein-fragment complementation screen inhibitory auto-regulatory domain k42a s289a double-mutant ser289 phospho-mimicking mutants cam auto-regulatory domain epo-dependent erythroblast formation coupled calcium/calmodulin binding cam auto-regulatory domains amp-activated protein kinase protein–protein interactions directly dap-kinase promotes autophagy a549-dfcp1-gfp cells a549 dfcp1-gfp cells multi-protein complex pro-apoptotic protein kinase anti-flag m2 beads graph height represents n-terminal catalytic domains initial low-affinity binding author contributions rsk-mediated survival signaling 5µci [γ-32p]atp anti-gaussia luciferase antibody unique calmodulin-independent mechanism central metabolic sensor pet28-tevh expression vector s289d/s289e mutant compared
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