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Title[redir]:
Structure of the activated Edc1-Dcp1-Dcp2-Edc3 mRNA decapping complex with substrate analog poised for catalysis - PMC
Description:
The conserved decapping enzyme Dcp2 recognizes and removes the 5′ eukaryotic cap from mRNA transcripts in a critical step of many cellular RNA decay pathways. Dcp2 is a dynamic enzyme that functions in concert with the essential activator Dcp1 and a ...
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Keywords {🔍}
dcp, doi, decapping, edc, substrate, structure, pubmed, rna, google, scholar, conformation, cap, active, fig, pmc, analog, complex, article, conserved, free, mrna, dcpdcp, nucleotide, nrd, activation, residues, data, protein, supplementary, tyr, enzyme, binding, structures, peptide, catalysis, site, transcribed, motif, helix, bound, shown, solution, edcdcpdcpedc, coactivators, catalytically, catalytic, glu, productbound, residue, pdb,
Topics {✒️}
substrate analog-bound edc1–dcp1–dcp2–edc3 protein complex edc1–dcp1–dcp2–edc3 edc1–dcp1–dcp2–edc3 decapping complex proline-rich dcp1-binding motif edc1–dcp1–dcp2–edc3 structure presented edc1–dcp1–dcp2–edc3 structure suggests apo pnrc2–dcp1–dcp2 complex pombe edc1–dcp1–dcp2 complex lys-rich patch result edc1–dcp1–dcp2–edc3 complex kl dcp1–dcp2–edc3 complex dcp2-dcp1 mrna-decapping complex conserved lys-rich patch kl edc1–dcp1–dcp2–edc3 pre-catalytic substrate-bound conformation lactis edc1–dcp1–dcp2–edc3 sp edc1–dcp1–dcp2 wt apo edc1–dcp1–dcp2 structure chimeric sp–hs structures lactis dcp1–dcp2–edc3 structure translation-libration-screw parameterization uk/pdbe/prot_int/pistart fo–fc omit map comprehensive python-based system temperature-sensitive growth defects26 dcp2-activating yagxxf motif yagxxf dcp2-activating motif sp dcp1–dcp2 complex dcp1 c-terminal extension unstructured c-terminal extension pmc beta search edc1–dcp1–dcp2 complex pombe edc1–dcp1–dcp2 substrate analog-bound state product-bound structures shows pombe product-bound structures core dcp1–dcp2 complex mirna-mediated gene silencing edc1–dcp1–dcp2–edc3 recent product-bound structures ni-nta affinity chromatography substrate analog-bound structures dcp1–dcp2 decapping complex x-ray data collection n-terminal regulatory domain recent dcp1–dcp2 structures substrate analog-bound structure substrate-bound conformation gave fo–fc difference map nonsense-mediated mrna decay
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